Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,247,134	A→C	Y467D (TAC→GAC)	lysP ←	lysine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,247,134	0	A	C	100.0%	39.8 / NA	13	Y467D (TAC→GAC)	lysP	lysine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (5/8);  total (5/8)

GGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATGGCAATCCC  >  NC_000913/2246896‑2247371                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              ggAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCTAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAaat                                                                                                                                                                                                                                   >  1:100426/1‑251 (MQ=255)    aCAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAAGTATCAATCCAAATGTTAAACTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTGAGCAAGGAAGGGAGGAAACTTATTTCTTAGCGTACTGCGTGAACTTCATTTCGCTGTAGCATACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCg                                                                                                                                                                                                                                           <  1:307258/240‑1 (MQ=255)             gATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTAGATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTGCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCGTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAAc                                                                                                                                                                                                                         <  1:302685/249‑1 (MQ=255)                               gATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACg                                                                                                                                                                                                      <  1:164877/250‑1 (MQ=255)                                          aaCGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTGCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTAcgc                                                                                                                                                                                          <  1:254931/251‑1 (MQ=255)                                                            aaCAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGGTCCTTTAATCAGCTTGGCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTa                                                                                                                                                                         <  1:193238/250‑1 (MQ=255)                                                                                                         tCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGAGATAATCAGac                                                                                                                           >  1:16759/1‑251 (MQ=255)                                                                                                                         gAAAANNACNNNTCNNTTGAGAGGGCTTAGCAAGGAAGGNAGGAAACTTATTTCTTATCGTTCTGCGGGNANTNCATTNNGCTNTAGCGTACGANGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGNGNGATACCAATATACGTTGCCGCTACGCCGCCCNCGTCAATAGTATCTTTCAgg                                                                                                                                                                >  2:263145/1‑198 (MQ=255)                                                                                                                         gAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCGTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACTTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAgg                                                                                                                                                                <  1:263145/198‑1 (MQ=255)                                                                                                                                      cGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTATTATCGTTCTGCGGGCACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCaa                                                                                                                                                                                                                                     >  1:198527/1‑116 (MQ=255)                                                                                                                                                                                            ggAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACg                                        <  1:3842/251‑1 (MQ=255)                                                                                                                                                                                                                    cGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGaaa                                                                                     >  1:281434/1‑182 (MQ=255)                                                                                                                                                                                                                                   tAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATGGCAATccc  <  1:9799/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              GGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATGGCAATCCC  >  NC_000913/2246896‑2247371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑