Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,120,969	G→A	L196L (CTG→TTG)	wcaJ ←	UDP‑glucose:undecaprenyl‑phosphate glucose‑1‑phosphate transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,120,969	0	G	A	100.0%	43.7 / NA	15	L196L (CTG→TTG)	wcaJ	UDP‑glucose:undecaprenyl‑phosphate glucose‑1‑phosphate transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/9);  total (6/9)

CCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGG  >  NC_000913/2120726‑2121199                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       ccGCACGGTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACAACCGGTACGCCGTTCATCTCTGCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGAGCAGCAGCACCGAACAGGTGGTGGCCGCCAGTTGATGGACCAGTTGGTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCGCGCGTTCGCGTCCTCGACCAACTGTTGc                                                                                                                                                                                                                                 <  1:105249/251‑1 (MQ=255)                             ggAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGAAGGTTACCCGCCCAGTCGTTAGAAAcgc                                                                                                                                                                                                     <  1:154359/251‑1 (MQ=255)                                     gcgTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCCGCTCAGTCGTTAg                                                                                                                                                                                                           <  1:95091/237‑1 (MQ=255)                                                                            ctTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACGTCAAAccn                                                                                                                                                          <  1:88381/247‑2 (MQ=255)                                                                                                        ggTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATc                                                                                                                           >  1:13341/1‑250 (MQ=255)                                                                                                                      gnATCAGCAGCCCCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCCGCCCAGTCGTTAGAAAcgc                                                                                                                                                                                                     >  1:277480/1‑162 (MQ=255)                                                                                                                      ggATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACgg                                                                                                                                                 >  1:7904/1‑214 (MQ=255)                                                                                                                               gcaCCGAACAGGTGGTGTCCGCCAGTTGAGGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCAGCGCGATATAGACGTTATGAATCTTGCCCTCTGTCGCGTCCCGGACCAACTGTTGCAGTTTACCCGCCCAGTCGGTAGAAACGCCGCCCTGTTTCGGGTCGTGGTAAACTCCCTCCGCTTCAAACCCTAACCACGGCTGGTTACGTTAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATcc                                                                                                   <  1:106240/251‑1 (MQ=255)                                                                                                                                   cGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGTGTCCTCGACCAACTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCGAAATCCCCCg                                                                                               <  1:301967/251‑1 (MQ=255)                                                                                                                                                     cAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGa                                                                                                                                      >  1:1737/1‑194 (MQ=255)                                                                                                                                                                                          cacaTTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAA‑TCCCCCGCCCCCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGcccccatt                                       >  1:273306/1‑247 (MQ=255)                                                                                                                                                                                                 gCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCTGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAg                                                                                                                                    <  1:232717/152‑1 (MQ=255)                                                                                                                                                                                                 gCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCCGCCAAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAAAGCCCACCACTTCAAACCCTAACAACGGCTGGTTACGTAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAAt                                  >  1:253890/1‑250 (MQ=255)                                                                                                                                                                                                                                cGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCGTGTTATATCCATGATTACGCAGCCAGCCCGCCCCAATTCGAATACACGAACGGCAAACCACCAGTCCGATGCTgg  <  1:134872/251‑1 (MQ=255)                                                                                                                                                                                                                                              cGACNNACTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACgg                                                                                                                                                 <  2:7904/94‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       CCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGG  >  NC_000913/2120726‑2121199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑