Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,645 G→A W282* (TGG→TAG)  thrB → homoserine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,6450GA94.7% 40.8 / ‑6.0 19W282* (TGG→TAG) thrBhomoserine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (10/8);  minor base C (1/0);  total (11/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

ATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTG  >  NC_000913/3401‑3887
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
attgcGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCGCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTggg                                                                                                                                                                                                                                              <  1:120001/251‑1 (MQ=255)
     gcACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGGATCTCCGGCTCCGGCCCGACCTTGTTCGCCCTGTGTGACAAGCCGGAAACCGCCCGGCGCGTTGCCAACTAGTTGGGTa                                                                                                                                                                                                                                            >  1:129879/1‑248 (MQ=255)
          gtcGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGAGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTAGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGACGACTAGTTGGGTAAGAACTAc                                                                                                                                                                                                                                    >  1:343386/3‑251 (MQ=255)
           gCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGTGTAAGAACTAcc                                                                                                                                                                                                                                   <  1:299289/251‑1 (MQ=255)
           gCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTAcc                                                                                                                                                                                                                                   >  1:327896/1‑251 (MQ=255)
                                                      ccTGAGCTTGCCGCGAAGCTGATGGAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGATTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCCGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATa                                                                                                                                                                                         >  1:108613/1‑250 (MQ=255)
                                                       cTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACACTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGATGGATAcg                                                                                                                                                                                       >  1:59299/1‑251 (MQ=255)
                                                                               aaGATGTTATCGCTGAACCCTACCGTGAACGGTTACGGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCGTGTTCTCTCTGTGTGACAAGCCAGAAACCGCCCAGCGCGTTGCCGACTAGTTggg                                                                                                                                                                                                                                              <  1:308882/172‑1 (MQ=255)
                                                                                                                              cggcaggcgcggcaggcgGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATATCCGGCTTCGGCCCGACCTTGTTCGCTCTGTGCGGCAAGCCGGGAACCGCCCAGCGCGGTGCCGACTCGTTGGGTAAGAACTAACTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTAc                                                                                                                                                                                        >  1:305431/1‑179 (MQ=255)
                                                                                                                               ggcaggcgcggcaggcgGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTAcc                                                                                                                                                                                                                                   >  1:23539/1‑135 (MQ=255)
                                                                                                                                                            ggCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCGGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCACAATCAGGAAGGTTTTGTTCATATTTGCCGGCtn                                                                                                                                                                                             <  1:208166/144‑2 (MQ=255)
                                                                                                                                                            ggCGCGATAGAGAGCGGAATCTCCGGCTCCGGCAAGACCTTGTTCGCTCTGGGTGACAAGCCGGAAGCCGCTGAGCGCGTTGCCGACTAGTTGGGGAAGAACTACCTGCAAAACAAGGAAGGTTGTGTACATANTTgnnnnngg                                                                                                                                                                                             >  2:208166/1‑137 (MQ=255)
                                                                                                                                                                                 tccggctccggcCCGACCTTGTTCGCTCTGCGTGCCACGCCGGAAACCGCAAAGCGCGTTGTCGACTAGTTGGGGCAAAAATACCTGAACAATCAGGAAGGATTTGTTCATATTTGCCGGCTGGATAcgggg                                                                                                                                                                                    >  1:154328/1‑130 (MQ=255)
                                                                                                                                                                                   cggctccggcCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGa                                                                                                                                                                                                                                         <  1:168100/77‑1 (MQ=255)
                                                                                                                                                                                                                             aaCCGCCCAGCGCGTTGCCGACGAGTTGGGTAAGAACTGCCTGCAAAATCAGGAAGGTTGTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAAGCTGGAAGTACACAACGAGCAGGGCAGCTTTGCGCAAGAGGTAACCAAGGGGTTGGGCAAAAATCAGGGGc                                                                       <  1:153989/197‑1 (MQ=255)
                                                                                                                                                                                                                                 gCCCAGCGNGNNNCNNACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTNATATTTGCCGGCTGGNTAnggcg                                                                                                                                                                                    >  2:250776/1‑84 (MQ=255)
                                                                                                                                                                                                                                          gTTGCCGANTAGTNGNNNAAGAANTACATGCAANATCAGGAAGGTTTTGTTCANATTTGcn                                                                                                                                                                                                  >  2:36589/1‑60 (MQ=255)
                                                                                                                                                                                                                                          gTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTTCCGGCTGGATACGCCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAgn                                                                                                                       <  1:36589/136‑2 (MQ=255)
                                                                                                                                                                                                                                             gCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGTGTTGGTCAAAAATCAGGGGCTTTTTTTTCCTCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTg  <  1:314983/250‑1 (MQ=255)
                                                                                                                                                                                                                                                   tngtngGGTANGAACNTCCTGCAAAATCAGGNAGGTTTTGTTCATATTGGCNNGNTGGACNcggcg                                                                                                                                                                                    <  2:154328/66‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
ATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTG  >  NC_000913/3401‑3887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑