Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,606,217	Δ1 bp :: IS186 (+) +6 bp	noncoding (65‑70/87 nt)	leuP ←	tRNA‑Leu

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2514274 =	NA (NA)	10 (0.650)	9/302	0.3	76.2%	noncoding (2/1345 nt)	IS186	repeat region
	?	NC_000913	= 4606222	3 (0.200)					noncoding (65/87 nt)	leuP	tRNA‑Leu
*	?	NC_000913	= 2515617	NA (NA)	16 (1.050)	10/300	0.3	88.5%	noncoding (1345/1345 nt)	IS186	repeat region
	?	NC_000913	4606217 =	2 (0.140)					noncoding (70/87 nt)	leuP	tRNA‑Leu

GACGACATAATCCAGCCATCGCTGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACG  >  NC_000913/2515378‑2515841                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                 gacgacATAATCCAGCCATCGCTGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGATGCATCGTCTGAACGAGGCACCGAGTCGTCGCATTCTTCAGAGGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCgg                                                                                                                                                                                                                       <  1:260483/251‑1 (MQ=16)        tAATCCAGCCATCGCTGGATTTCCCCTCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGATGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTAGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGttttttt                                                                                                                                                                                                                >  1:134288/1‑251 (MQ=255)             cAGCCATCGCTGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGcc                                                                                                                                                                                                           >  1:12322/1‑251 (MQ=39)                                                                                                             ggTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGCAGCACGCGAGGCATCGTCTTAACGACGCACCGAGGCGTCGCATTCTTCAGCTGGTTCAACCCTTAAGTTCGCGCTTATGGGATCATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTCATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCg                                                                                                           >  1:288877/1‑251 (MQ=25)                                                                                                                  ccATTCGGGCGACAGTATCCCTGACAGCCTACAAAACGCAATCGAAGAACGCGAGGCATCGTCTTAACGATGCACCGAGGCGTCGCATGCTTCAGATTGTTCAACCCTTAATTTATCGCGTATGGGATAATTACCCGGTTTTTTTACGCCTGTTAGTCAGCTAATGTCTGCTGGCGATCAATCGAGTACCCTTGCAGCTATGGCACCCCTAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACaaa                                                                                                      <  1:345027/251‑1 (MQ=21)                                                                                                                       cGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGTACCGAGGCGTCGCATGCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCcg                                                                                                 <  1:21187/251‑1 (MQ=255)                                                                                                                                    cccTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCCTTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCa                                                                                     >  1:187852/1‑250 (MQ=255)                                                                                                                                     ccTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGAc                                                                                             >  1:290230/1‑241 (MQ=255)                                                                                                                                                      cGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGATTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAg                                                                  <  1:58137/251‑1 (MQ=255)                                                                                                                                                        cAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGCTGGTTAAACCCTTAAGTTAGCGCTTATGGGATAATTCCCGGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATCCCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGcc                                                                    >  1:304285/1‑247 (MQ=255)                                                                                                                                                                                  tAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGc                                       >  1:147746/1‑250 (MQ=255)                                                                                                                                                                                                 gCGTCGCATTCTTCAGCTGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTg                       >  1:116566/1‑251 (MQ=255)                                                                                                                                                                                                        aTTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCATATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAATCTCCTCGACCacac                                                                                        <  1:81403/179‑1 (MQ=255)                                                                                                                                                                                                        aTTCTTCAGATGGTTCAACCCTTAAGNTAGCGCNNATNGGATAATTCCCCGGTTTTTNTANNNCNNNTNCTCAGNTANNGGATGCGGGNGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCNGCTGCTGTTNNGTCTCGACAAACTCCGCGACCNCACNNANNgnaagnggn                                                                          >  2:81403/1‑185 (MQ=255)                                                                                                                                                                                                              cAGATGGTTCAAACATTAAGTTAGCGCTCATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGAAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTg                       >  1:300043/1‑238 (MQ=255)                                                                                                                                                                                                                      tCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTGTCGcn                                                                    <  1:86387/185‑2 (MQ=255)                                                                                                                                                                                                                                          tNNGGGATANNTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGATTATGc                                                                                                                                      <  2:12322/99‑1 (MQ=255)                                                                                                                                                                                                                                               gATAATTCCCCGGTTTTTTTACGCCTTTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATGCCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCGGCTGCTGTTGCGTCGCTACAAACTCCGCGACCACACTCAATGACTTCCCTTTCGCCAGATCGGTAAGTGATCTCACAATCATCACATCCAGCGTGTTCGTGACAATAGCTTTCACAAAGAcn  <  1:65237/226‑2 (MQ=255)                                                                                                                                                                                                                                               gATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATTCCCGTGCAGATAGTTCACCCCGAGCTTATGCAATAGCGTCTGCTGCTGTTCCGTCACGACAAACTCCGCGACCACACTCAATGACTTCACtttgaatgccgtcag                                                            <  1:341611/168‑13 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                 GACGACATAATCCAGCCATCGCTGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACG  >  NC_000913/2515378‑2515841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.