| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,815,883 | +C | coding (667/687 nt) | rph ← | truncated RNase PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,815,883 | 1 | . | C | 94.4% | 48.8 / ‑3.0 | 18 | coding (667/687 nt) | rph | truncated RNase PH |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (7/10); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01 | |||||||||||
ATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCC > NC_000913/3815646‑3816115 | aTTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTGCTACAGACAAAAAAAAGGCGACGCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCggg < 1:171070/244‑1 (MQ=255) ggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTg > 1:312992/1‑250 (MQ=255) ngTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc > 1:86852/2‑251 (MQ=255) cGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc > 1:179620/1‑251 (MQ=255) ctAAGCGCAAATTCAAAAAACGGGCGCTGATATGGTATCATGCCCTCGCTCCTCATCTTACTTTGCTACAGAAAAAAAAAAGTCGACTCGTCAGTCGCCTGAAAAATCAGCTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCGAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGc < 1:264052/214‑1 (MQ=255) aGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCGCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatc < 1:201962/251‑1 (MQ=255) atGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCg < 1:271459/185‑1 (MQ=255) cctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTACCAATAAGTTTGCCCGCGACGCCTCCTGCGTCGATACAATGGATTCCATTCC‑CCCTCGGGCCCGCGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACt > 1:175157/1‑177 (MQ=255) tcATCTTACTTTGCTACAGACAAAAAAAAGGCGACTCATCAGTCGACTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGATCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg < 1:53279/184‑1 (MQ=255) tACTTTGCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGGCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCg < 1:118115/247‑1 (MQ=255) aCAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAGTCAGTTCGCCAGCGCCGCCTTCTGCGTCGCTTAAAGGGATTCGATTCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGATGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTCCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCATAGTCTTCAACGTATTCCAGATCGCAAACCGCTTc < 1:28329/249‑1 (MQ=255) cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCCTCTGCGTCGCTCCAATGGATTCGATTCCACC‑TCGGGCCAGAGCCACCAAGATGAGTAGCTCTTCCTGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTACTGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGAc > 1:49795/1‑251 (MQ=255) aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgcn < 1:86140/132‑2 (MQ=255) nccgccTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGAGCCAACAAGCTGAGTAGCTATCCATAGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCACCGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGt > 1:69557/2‑251 (MQ=255) nccTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGc > 1:70234/2‑155 (MQ=255) ccTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCGCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTc < 1:176443/251‑1 (MQ=255) gCTACAATGGATTCGATTCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGCTGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGCTCGCAAACCGCTTCGCCGTTCACAATTCCGACAGACACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGtt > 1:133975/1‑251 (MQ=255) aCAATGGATTCGATTCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAGCGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCGCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTGCGCCGTTCACAATTC‑GACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTcc < 1:356572/251‑1 (MQ=255) | ATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCC > NC_000913/3815646‑3816115 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |