| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,773,173 | Δ1 bp | coding (893/1914 nt) | mtlA → | mannitol‑specific PTS enzyme IICBA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,773,173 | 0 | G | . | 96.3% | 98.0 / ‑2.3 | 27 | coding (893/1914 nt) | mtlA | mannitol‑specific PTS enzyme IICBA component |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (10/16); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01 | |||||||||||
AGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTG > NC_000913/3772949‑3773390 | aggtATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCGCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGac < 1:114932/251‑1 (MQ=255) gggctgctGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGAGCGGATATCCTCCACTTCCTGGGGGGTTTCAAAAAAATCAACTTCCCGTATCGGCTGATGAATCCGCGTCTGATCCTCACAGTAATCCTCGGAGGTGTGACTGGCGGGTTCACGCTTGCACTCCTGGGTGGGTGG‑CTGGCTTCTTCGGAATATCCGG‑TTTTATCCTTGCTGTAATGGCGATGaccaccacagg > 1:177282/3‑241 (MQ=255) gctgctGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGc < 1:268787/251‑1 (MQ=255) ntACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGacac > 1:214188/2‑232 (MQ=255) gTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGCTGacac > 1:161763/1‑232 (MQ=255) cgcTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTAACGTATGTGCTGATGAATCCGCGTCTGATCCTCGCCGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGGGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTgcggc > 1:258137/1‑251 (MQ=255) aCAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCGGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TGTTATCCTTGCTGTACTGGCGAGGACTCCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGc < 1:164868/251‑1 (MQ=255) ccgcgggcggTGCGGCAATCATCCACTCCCTGGGGTGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGG‑CGGTGGGCTGGTTTCTCCTGCATCTCCGG‑TTTTATCCTTGCTGGACTGGCGATGACACCAAAAGGTGc < 1:230179/205‑1 (MQ=255) gCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACAGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTctct > 1:76072/1‑251 (MQ=255) cACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCGTCTCCGG‑TTTTATCCGTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCGAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTg < 1:350823/251‑1 (MQ=255) aCTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGCGCTGATGAATCCCCGTCTGATCCTCTCAGTCATCCTCGGCGGTCTGACTGGCGTGCTCACGCTGAATATCCTGGG‑AGGTGGCCTGGTTTCTCCGGCCTCTCCGG‑TTTTTTCCTTGCTGTACTGGCGCTGACCCCCAAAGGt > 1:360513/1‑181 (MQ=255) tCCTGGGGGGTATCCACGAAATCTACTGCCCGTATGTGCTGATGAATCCGCGTCTGAGCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACGGGCGATGACACCAAAAGGTGCTTTCTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCt < 1:315833/249‑1 (MQ=255) nnnnnnntnccnngaTGNGCTGATGAATCCGCGTCTGATCCTNGCAGGCATCCTCGGCGGTATGACTGGCGTGTNCNCGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTNATCCTTGCTGNACTNNCGATNANANCAAAAGGTGCTTACTTCGCTAACATCGCGNGTGTGTGTGCGGCGATGGCTGTCNNCTNNNTTNNCTCTg < 2:256007/206‑1 (MQ=255) tNNNNNATGNGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGacac < 2:214188/143‑1 (MQ=255) aaTCCGCGTCTGATCCTCGCAGTCATCCGCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGc < 1:254907/175‑1 (MQ=255) ntCTGATCCTCGCAGTCATCCTCGGCGGAATGACTGGCGTGTTCACGCTGACACTCCTGGG‑CGGGGGTCTGGTTTCTCCGGCCTCACCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAAGTGCTTCCTTCGCTAACATCGCGGGTGTGGGTGCGGCGGTGGc > 1:43519/2‑168 (MQ=255) ctgcggTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTGCTGGCGAGGACACCAAAAGAGGCTTGCTTCGCTAACATCGCGGGTGTGTGTTCGGCGATGGGTGTCTCCTTCGTTGGCTCTGCTATTTGGCTTAAATCCAGCATAGTGAAATAAGAAGATGATTTTGAAGCAGCAACTCGTCGTATGCAGGTCATGAAAGCTGAg < 1:234861/247‑1 (MQ=255) gTATGNNTGNNNTGNNCACGCTGACTATCCTGGG‑CGGTGNTCTGGTTTCTCCGGGCTC‑CCCGGTTTTATNCNTNCTGTNNTGGNGATGACACCANAAGGGGCTTACTTCGCCAACATCGCGGGTGGGGGCGCGGCAACGGCTGNGNTCTTCGCTGTCGCTGCTATTTAGCTGAAAAANAGTAAAGCGAAAGAAAGAGATGAGNTTGan > 2:254705/1‑207 (MQ=255) gTATGNNTGNNNTGNNCACGCTGACTATCCTGGG‑CGGTGNTCTGGTTTCTCCGGCATCTCCGG‑TTTTATNCNTNCTGTNNTGGNGATGACACCANAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGNCNCCTTCGTTGTCTCTGCTATTTTGCTGAAAACNAGCAAAGTGAAAGCAGAAGATGATNTTGAnncancn > 2:254706/1‑213 (MQ=255) ggCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGcn < 1:221114/77‑2 (MQ=255) ggCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCg > 2:221114/1‑77 (MQ=255) tNGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCNTTGCNGNNCTNGNGANGacac < 2:161763/62‑1 (MQ=255) ggg‑CGGTGGTCTGTTTTCTCCGTCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAATAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGAtgtg < 1:254705/249‑1 (MQ=255) ggg‑CGGTGGTCTGGTTTCTCCGGCATCGCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGAGGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGAtgtg < 1:254706/249‑1 (MQ=255) tnggNNTCTCNGGCATCTCCGG‑TTTTATCCTTGCTNTACTGGCGATGACACCAAAAGGTGCTTACTTNGNTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCNGCTATTTTGCNGAANNCCAGNANANTGAAATAAGAAGATTATATTGAAGCAGCANCTCGTCGTATGCAGGACATGAAANNTGNNNCTNNAGgcg < 2:258137/211‑1 (MQ=255) tctcCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGNAAANNNNnagnnnnnn > 2:213912/1‑144 (MQ=255) tctcCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAgn < 1:213912/205‑2 (MQ=255) | AGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTG > NC_000913/3772949‑3773390 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |