| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,207,549 | Δ1 bp | coding (179/606 nt) | ttdB → | L(+)‑tartrate dehydratase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,207,549 | 0 | G | . | 86.7% | 40.4 / 0.7 | 15 | coding (179/606 nt) | ttdB | L(+)‑tartrate dehydratase subunit beta |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base . (7/6); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
CGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGAC‑CGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGA > NC_000913/3207303‑3207777 | cgtcgCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGCGCGCGTTATGTAAAAGATCCTGACAACCCCGATCCAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGTTCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAAAGGCAAAGCGATTTTCAACGCTGcccc > 1:55487/1‑250 (MQ=255) ggCACGCTGCTGTTTCATGCCGATCTCCCCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACACCCCCGATCAAAGCTGAAGATCGGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGGGACCTGCCGCGACGTTTGTCACCGCCGGTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACTCTG‑CCCCATCGTgc < 1:60962/251‑1 (MQ=255) ggTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACCCCCGGAGCGCGTTAGGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATGCGCGTCGGCGATGTGATCTACCGGCCCGGTACGCTGGTGACCTGCCGGGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGc < 1:170482/251‑1 (MQ=255) gAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGAGCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCTCGACGTTTGTCACCGCTGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGC‑AGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGGCTCCGGCTGCCCGACAACCAGTATg < 1:270608/251‑1 (MQ=255) aaGATCTGCAAGATATTCGCGTCGGCNATGTGANNTANCTGACCGGTACGCTGGTGANCTNNNGNNNCNTTTGTNACNNCCGTTTGATNGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGC‑NGCCCCATCGNNCGCACAAACGGCGACAAATGGNAGANNGNNTNCGTNggn > 2:79973/1‑192 (MQ=255) ntGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGAGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTgc > 1:39282/2‑150 (MQ=255) aGATATTCGCGTCGGCGATGTGATCTNCCTGACNNGTNCGCTGGTGACCTGCCGCGANGTNNNTNNNCNCCGTTNGANNGAACTGAAANGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGNGCGCAAAAANNGCGACAAATGGGAGATGGTCTNCGTNNGNNCNACANcc > 2:82002/1‑192 (MQ=255) gTGACCTGCCGCGACTTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGAC‑CGAAGAAGGCTGc < 1:82002/251‑1 (MQ=255) ccTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGAGGTTGGCAAAGGGGGTATGGGGCCGCTGAC‑CGAAGAAGGCTGcc > 1:82845/1‑248 (MQ=255) gTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCAAGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAAt > 1:307912/1‑105 (MQ=255) cACCGCCGTTTGATCGAACTGAAACGTCCGTCCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTTTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGACCGTGAATTTATTGAGCAGACGGGCGTTAATCTGGTGGTTGGCAAAGGTGTTATGGGGCCGCGGAC‑GTAAGAAGGTTCCCAGAAATTCAAGGCGCTACa < 1:110009/250‑1 (MQ=255) ccgccgTTTGATCGCACTGCAACGTCCGATTCCTTAAGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCCTCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACAGGCGGGAACCTGGGGGTTGGCAACGGGGGTATGGGGCCGCT‑ACACGACGAAGGCTGCCAGAAATTCAAGGCGTTACAttg > 1:302305/1‑249 (MQ=255) cTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGAGATTTTCCACGGTG‑CCCCATCGTGCGCGAAAACGGCGACAACTGGGAGATGGACTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGATTTGAAAGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGATGGCAAAGGTGGTATGGGGCCGATGCC‑CGAAGAAGGCTGACAGAAATTCAAGGCGCTACATGTGATTTTACCGGCAGGc > 1:264413/1‑251 (MQ=255) naaaCGTNCGATCCCTTACGATCTCAANGGCAAAGCGNTTTGCNNNGNTG‑CCNCATCGTgc < 2:39282/60‑1 (MQ=255) gAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGAC‑CGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGc < 1:79973/249‑1 (MQ=255) ngCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGAGTGAAACTGGTGGTTGGCAAAGGTGGGATGGGGCCGCTGAA‑CGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAAACCAGGGGGa > 1:33370/2‑250 (MQ=255) | CGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGAC‑CGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGA > NC_000913/3207303‑3207777 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |