Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,183,557 Δ1 bp intergenic (‑6/+256) yqiD ← / ← ribB protein YqiD/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,183,5570T.94.7% 64.0 / ‑1.7 19intergenic (‑6/+256)yqiD/ribBprotein YqiD/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base . (9/9);  total (10/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

GACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGAAATAACCCACCACAACCAGAGCAATCAATACAATCCAGTACCAGGCGATAAACATCCTTTTACCCTTAATATTTAACAATACTAAACATTTAGCGTATAAATTTCACATATCCTTTTTCGGATATATTCATATGGTCGCATAATAACGGAACAATTATATGATTTTGATCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTT  >  NC_000913/3183353‑3183780
                                                                                                                                                                                                            |                                                                                                                                                                                                                               
gACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTACGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGCAACGCCCGCCAATAACGTTTCAAATGCCGGCAATAACCCACCACAACCAGAGCAATCAATACAATCCAGTACCAGGCGATAAACATCCTTT‑ACCCTTAATATTTAACAATACTAAACATTTAGCGTATAAATTTcac                                                                                                                                                                                   >  1:204907/1‑250 (MQ=255)
   aaCTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGAAATAACCTACCACAACCAGAGCAATCAATACAATCCAGTACCAGGCGATAAACATCCTTT‑ACCCTTAATATTTAACAATACTAAACATTTAGCGTATAAATTTCACatat                                                                                                                                                                               >  1:350561/1‑251 (MQ=255)
     cTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTTTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGAAATAACCCACCACATCCAGAGCAATCAATACAATCCAGTACCAGGCTATAAACATCCTTT‑ACCCTTAATATTTATCAATACTAAACATTTAGCGTATAAATTTcaca                                                                                                                                                                                  <  1:291449/246‑1 (MQ=255)
                                                                                                     ctGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGAAATAACCCACCACAACCAGAGCAATCAATACAATCCAGTACCAGGCGATAAACATCCTTT‑ACCCTTAATATTTAACAATACTAAACATTTAGCGTATAAATTTCACATATCCTTTTTCGGATATATTCATATGGTCGCATAATAACGGAACAATTATATGATTTTGATCATAATGAAAAAGAATGCCTGATATTAATGAAATTTtc                                                                               <  1:83244/249‑1 (MQ=255)
                                                                                                     ctGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGAAATAACCCACCACAACCAGAGCAATCAATACAATCCAGTACCAGGCGATAAACATCCTTG‑ACCCTTAATATTTAACAAt                                                                                                                                                                                                              <  1:137100/122‑1 (MQ=255)
                                                                                                             gCGAAACGCCCGACAATAACGTTTCAAATGCAGGAAATAACCCACCACAACCAGAGCAATCAATACAATCCAGTACCAGGCGATAAACATCCTTT‑ACCCTTAATATTTAACAATACTAAACATTTAGCGTATAAATTTCACATATCCTTTTTCGGATATATTCat                                                                                                                                                           >  1:301041/1‑165 (MQ=255)
                                                                                                               gAAACGCCCGACAATAACGTTTCAAATGCAGGAAATAACCCACCACAACCAGAGCAATCAATACAATCCAGTACCAGGCGATAAACATCCTTT‑ACCCTTAATATTTAACAATACTAAACATTTAGCGTATAAATTTCACATATc                                                                                                                                                                              >  1:136045/1‑144 (MQ=255)
                                                                                                                 aaCGCCCGACAATAACGTTTCAAATGCAGGAAATAACCCACCACAACCAGAGCAATCAATACAACCCCGTACCAGGCGATAAACATCCTTT‑ACCCTTAATATTTAACAATACTAAACATTTAGCGTATAAATTTCACATATCCTTTTTCGGATATATTCATATg                                                                                                                                                        >  1:51726/1‑164 (MQ=255)
                                                                                                                    gCCCGACAATAACGTTTCAAATGCAGGAAATAACCCACCACAACCAGAGCAATCAATACAATCCAGTACCAGGCGATAAACATCCTTT‑ACCCTTAATATTTAACAATACTAAACATTTAGCGTATAAATTTCACATATCCTTTTTCGGATATATTCATATGGTCGCATAATAACGGAACAATTATATGATTTTGAt                                                                                                                     <  1:2830/196‑1 (MQ=255)
                                                                                                                      ccGACAATAACGTTTCAAATGCAGGAAATAACCCACCACAACCAGAGCAATAAATACAATCCACCACCAGGCGATAAACATCCTTT‑AACCTTAATCTTTAACAATACTAAACATTTAGCGTATAAATTTCACATATCCTTTTTCGGATTTATTCATATGGTCGCATAATAACGGACCAATTATATGATTTTGATCATAATGAAAAAGAATGACTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCgg                                                             >  1:346560/1‑250 (MQ=255)
                                                                                                                       cGACAATAACGTTTCAAATGCAGGAAATAACCCACCACACCCAGAGCAATCAATACAATCCAGTACCAGGCGATAAACATCCTTT‑ACCCTTAATATTTAACACTACTAAACATTTAGCGTATAAATTTCACATATCCTTTTTCGGATATATTCATATGGTCGCATAATAACGGAACAATTATATGATTTTGATCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTATCATAGAAAGAGAATCGGa                                                            >  1:46963/1‑250 (MQ=255)
                                                                                                                           aaTAACGTTTCAAATGCAGGAAATAAGCCACCACAACAAGAGCAATCAAAACAAACCAGTAACACGCGAAAAACATA‑ATTTACCCTTAATCTTTAAAACTACTAAACAATTAGCGTATAAATTTAACATATCATTTTTCGGATATCTTCATATGGTCGCATAATAACGGAACAATTATATGATTTTGATCATAATGAAAAAAAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTa                                                        >  1:162613/1‑250 (MQ=255)
                                                                                                                                           cAGGAAATAACCCACCACAACCAGAGCAATCAATACAATCCAGTACCAGGCGATAAACATCCTTT‑ACCCTTAATATTTAACAATACTAAACATTTAGCGTATAAATTTc                                                                                                                                                                                     <  1:25362/109‑1 (MQ=255)
                                                                                                                                            aGGAAATAACCCACCACAACCAGAGCAAACAATACAATCCAGTACCAGGCGATAAACATCCTTT‑ACTCTTAATATTTAACAATACTAAACAATTAGCGTATAAACTTCGCATATCCTGTTTCGGATATAGTCATATGGTCGCATAATAACGGAACAAGTATATGATTTTGATCATGATGTAAAAGTATGCCTGATATTAATGAAATGTTCTCATAGAAAGAGAAGCGGAATTAACATCGTGCATAGCAggg                                      <  1:232046/251‑1 (MQ=255)
                                                                                                                                             ggAAATAACCCACCACAACCAGAGCAATCAATACAATCCAGTACCAGGCGATAAACATCCTTT‑ACCCTTAATATTTAACAATACTAAACATTTAGCGTATAAATTTCACATATCCTTTTTCGGATATATTCATATGGTCGCATAATAACGGAACAATTATATGATGTTGATCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCAt                                             <  1:110251/243‑1 (MQ=255)
                                                                                                                                              gAAATAACCCACCACAACCAGAGCAATCAATACAATCCAGTACCAGGCGATAAACATCCTTT‑ACCCTTAATATTTAACAATACTAAACATTTAGCGTATAAATTTCACATATCCTTTTTCGGATATATTCATATGGTCGCATAATAACGGAACAATTATATGATTTTGATCATAATGAAAAAGAATGcc                                                                                                  <  1:272023/189‑1 (MQ=255)
                                                                                                                                                   aaCCCACCACAACCAGAGCAATCAATACAATCCAGTACCAGGCGATAAACATCCTTT‑ACCCTTAATATTTAACACTACTAAACATTTAGCGTATAAATTTCACATATCCTTTTTCGGATATATTCATATGGTCGCATAATAACGGAACAATTATATGATTTTGATCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAAt                                >  1:182977/1‑250 (MQ=255)
                                                                                                                                                                     aaatcaatACAATCCAGTACCAGGCGATAAACATCCGTT‑ACCCTAAATATTAAACAATACTAAACATTTAGCGAAAAAATTTCACATATCCTTTTTCGGATATATTCATATGGTCGCATAATAACGAAACAATAAAATGATTTTGATCAAAAAGAAAAAGAATGCCTGATATAAATGAAATTTCCTCAGAAAAAGAGAAGCTGAATTAATATCTTGCATAGCAGGGAAAAAAAAAAACCGAAGCCTGCTAc             <  1:136968/250‑1 (MQ=255)
                                                                                                                                                                                aTCCAGTACCAGGCGATAAACATCCTTT‑ACCATTAATATTTAACAATACTAAACATTTAGCGTATAAATTTCACATATCCTTTTTCGGATATATTCATATGGTCGCATAATAACGGAACAATTATATGATTTTGATCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGttt  >  1:131511/1‑251 (MQ=255)
                                                                                                                                                                                               aTNAACATNCTTT‑NCCCTTAATATTTAACNATACTAAACATTTAGCGTANNANTTTCANATATc                                                                                                                                                                              <  2:136045/64‑1 (MQ=255)
                                                                                                                                                                                                            |                                                                                                                                                                                                                               
GACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGAAATAACCCACCACAACCAGAGCAATCAATACAATCCAGTACCAGGCGATAAACATCCTTTTACCCTTAATATTTAACAATACTAAACATTTAGCGTATAAATTTCACATATCCTTTTTCGGATATATTCATATGGTCGCATAATAACGGAACAATTATATGATTTTGATCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTT  >  NC_000913/3183353‑3183780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑