Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,111,2590G.90.9% 31.5 / ‑2.2 11coding (718/900 nt)rfbDdTDP‑4‑dehydrorhamnose reductase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (2/8);  total (3/8)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCC  >  NC_000913/2111103‑2111508
                                                                                                                                                            |                                                                                                                                                                                                                                                         
cATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCACAGTTCTGCTGACATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATg                                                                                                                                                                             >  1:145166/1‑234 (MQ=255)
                            ccAGTCAGGCAAGACAAGCGCAAAGTTCTTCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGGACCAGTTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGc                                                                                                                                <  1:315861/251‑1 (MQ=255)
                            ccAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAgn                                                                                                                                                                     <  1:68733/214‑2 (MQ=255)
                                  aGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGc                                                                                                                                            <  1:13989/233‑1 (MQ=255)
                                         aCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCGTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCt                                                                                                                                                                                     <  1:130774/185‑1 (MQ=255)
                                               gcAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCGTGTTGAGTGCAAGGG‑AATGCCTGCTTTgcgcg                                                                                                                                                                                                                                          <  1:122022/126‑1 (MQ=255)
                                                                              ggCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGATTGTTGAGTG‑AAAGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGACAGCAGTTCAGCACCTGTTGGCGCACCAAACt                                                                              >  1:181259/1‑251 (MQ=255)
                                                                                              acgacgAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGCGTGCAAGGG‑CATGCCTGATTTGCGCGCCTCTTCAAACACCAGCACAGCATAATCGTACCAGGTTGTGGTACCCCTGGCTACCACCTGGTACAAGCCTGCGACATCAGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGc                                                              >  1:176760/1‑251 (MQ=255)
                                                                                                        ggTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCGTCAAAAACCAGCGCATCATAATCGTACCAGGTTGTGGTACCACTGGCGACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATcagccag                                                                                                           <  1:259525/196‑1 (MQ=255)
                                                                                                                                        gAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGc                    <  1:333942/251‑1 (MQ=255)
                                                                                                                                          gatttttgAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCGTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGTGACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGTTTTATTCAGTGCGACACGAAGGGCATGTGCTGTACAATCAGCCAGCAGGTTAGCACCTGTTGTCGCACCAAACTGATCGTTAATAACCTCTAATTCCTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGa                  <  1:4000/246‑1 (MQ=255)
                                                                                                                                                           ggAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTAtttccttttcn  <  1:65700/251‑2 (MQ=255)
                                                                                                                                                            |                                                                                                                                                                                                                                                         
CATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCC  >  NC_000913/2111103‑2111508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑