Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,907,451 IS2 (+) +5 bp coding (137‑141/144 nt) yobF ← DUF2527 domain‑containing protein YobF

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1651548NA (NA)25 (1.300) 17/276 0.1 92.3% noncoding (706/706 nt) IS2 repeat region
?NC_000913 1907451 = 2 (0.110)coding (141/144 nt) yobF DUF2527 domain‑containing protein YobF
* ? NC_000913 = 19074551 (0.050)21 (1.090) 19/276 0.0 95.3% coding (137/144 nt) yobF DUF2527 domain‑containing protein YobF
?NC_000913 = 2070271 NA (NA)noncoding (1/1331 nt) IS2 repeat region

GGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCTGAAAAATTTCTGGCACTGAAAGAAACTGAAGTCGCC  >  NC_000913/1651305‑1651718
                                                                                                                                                                                                                                                   |                                                                                                                                                                          
ggAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGCAATATAGGGGCAAATCCACAGGTg                                                                                                                                                                      >  1:321602/1‑250 (MQ=17)
            gTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGctcctc                                                                                                                                                         >  1:171131/1‑251 (MQ=38)
              aaCGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGTAGAGGCGTTCGAGCATTATAAAGAATGGCATCCGCATAGTGCGCTGGGATATCGCTCGCCACGGGAATATCTGCGGCAGCCGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGa                                                                                                                                                                     <  1:199617/237‑1 (MQ=12)
                              gCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATaa                                                                                                                                        >  1:397888/1‑250 (MQ=255)
                                                  cGTGACGACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCGCGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTTAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATg                                                                                                                   <  1:294909/251‑1 (MQ=255)
                                                                                  ngggTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTgg                                                                                     >  1:315399/2‑249 (MQ=255)
                                                                                          cGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTATGTTACGCAAGAAAAACTGGTGGTTTCGACTATGCAAAAGAGTTTCCCTCCTCACGCAATCTGGAAAAATTTgg                                                                           <  1:425393/251‑1 (MQ=255)
                                                                                                 aaaGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTGATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTAcn                                                                                                                                              <  1:46765/177‑2 (MQ=255)
                                                                                                            cAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGc                                                                                                                                             <  1:1465/167‑1 (MQ=255)
                                                                                                                                        gNATCCGCCTAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCTGAAAAAttt                             >  1:392627/1‑251 (MQ=255)
                                                                                                                                           tCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATCACAGCTGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGCAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCTGAAAAATTTCTg                          >  1:190593/1‑251 (MQ=255)
                                                                                                                                            ccGCATAGTGCGCTGGGTTAGCGCTCGCCACGGGAATATCTGCGGCAGCTGGCTTGGAATGGGTTAAGTGACAACAGATGTCTGTAAATATAGGGGCAATTCCGCAGGt                                                                                                                                                                       <  1:417310/109‑1 (MQ=11)
                                                                                                                                                aTAGTGCGCTGGGTTATCGCTCTCCACGTGGATATCTGCTGCAGCGTGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGGGAAGTACGCTCCTCTGTTGGTTACGCAATAAAAGCTGGTGTTGTCGGCTATGCAAAACAGTGTCCCTCCTCACGTAATCGGGAAAAATTTTGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCTGAAAAATTTCTg                          <  1:373484/246‑1 (MQ=25)
                                                                                                                                                            gTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAAt                                                                                                                                          >  1:276461/1‑122 (MQ=255)
                                                                                                                                                                tcgctcgcCGCGGGAATATCTGCGGCTGCTGGCTTGTAATGGGTTAAGTGATAACATGTGGGTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCtgtttgtt                                                                                                                                                 <  1:237566/111‑1 (MQ=18)
                                                                                                                                                                 ngctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAAAAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCTGAAAAATTTCTGGCACTGAAAGAAACTGAAGTCg    >  1:299284/2‑251 (MQ=255)
                                                                                                                                                                   ctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCTGAAAAATTTCTGGCACTGAAAGAAACTGAAGTCGcc  >  1:199719/1‑251 (MQ=255)
                                                                                                                                                                                         nngCNNNNNTGTNATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTgg                                                                                     <  2:315399/144‑1 (MQ=255)
                                                                                                                                                                                              ggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGANATATANGNGNNAANNCACAGGTg                                                                                                                                                                      <  2:340950/60‑1 (MQ=14)
                                                                                                                                                                                              ggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCCCAGGTg                                                                                                                                                                      >  1:340950/1‑60 (MQ=255)
                                                                                                                                                                                                         ngTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACNGTTNCCCTNCTNACGCAATCTGGAAAANTTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCTGANNANNNTNTGGCACTg                    <  2:347459/194‑1 (MQ=255)
                                                                                                                                                                                                         ggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCATCTGTTTGTTACGCAATAAAAACTGGTGTTTTAGACTATGCACAACAGTTTCCCTCCTCACGCAATATGGAAAAATTTGGTGAGGCCCGACAAGATTTAAACATAAAAGAACTGGCTGAAAAATTTCTGGCACTg                    >  1:347459/1‑195 (MQ=255)
                                                                                                                                                                                                                          tGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACTCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTgg                                                                                     <  1:257678/113‑1 (MQ=255)
                                                                                                                                                                                                                           gNNTGGAAANNTAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCcctcc                                                                                                  <  2:25407/99‑1 (MQ=255)
                                                                                                                                                                                                                                           ncAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTACTCACGCAATCTGGAAACATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCTGAAAAATTTCTGGCACTGAAAGAAAc            >  1:51642/2‑169 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                          
GGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCTGAAAAATTTCTGGCACTGAAAGAAACTGAAGTCGCC  >  NC_000913/1651305‑1651718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.