Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,429,1600CG61.5% 6.8 / 12.4 13L38V (CTG→GTG) stfRputative prophage side tail fiber protein StfR
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base G (3/5);  total (6/7)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.28e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTT  >  NC_000913/1428953‑1429366
                                                                                                                                                                                                               |                                                                                                                                                                                                              
gTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCCGCCAACACCGTGTTATGACAGCCCGCCGGTTCAAGCGGGCTTTTTTGTGGGGTGGATATGGCAGTACAGATTTCCGGTGTACTGACAGACAGCACCGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGAAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATgg                                                                                                                                                                      >  1:80572/1‑250 (MQ=255)
                                      aGGTAACACAGTGTGATGACAGCCCGCCGTAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTTCTGAAATACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACATCACCACGGTGGTGGTTAACACTGTGGGCTCAGATAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAgtgtcatcctgcaggttg                                                                                                                               <  1:74078/251‑18 (MQ=255)
                                                                                                   gCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAg                                                                                                                                                     >  1:65632/1‑168 (MQ=255)
                                                                                                      ggaaaGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACTGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCGTGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCt                                                               <  1:81215/249‑1 (MQ=255)
                                                                                                                          naaaGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGcc                                            >  1:41961/2‑250 (MQ=255)
                                                                                                                          gAAAGACTGCACAGGAAAACCGGTACAGAATTGCACAATCCATGTGAAAGCAAAACGGAACAGCACCACGGTGGTGGTGAACACGCGGGCCTCAGAAAATCCGTATGAAGCCGGGCGTTACAGCAGTGAGGTTGGTTACGGTCAGTACAGCTTTAGTCTGTGGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGc                                             <  1:122941/249‑1 (MQ=255)
                                                                                                                                  gCACAGGAAAACCTGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTTATCCTGCAGGTTGACGGTTTGCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACggan                                   <  1:18445/251‑2 (MQ=255)
                                                                                                                                          aaaCCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTAACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTGTCCACCAGCGCACGCCGGGACCATCACCGTGGATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGc                           <  1:166676/251‑1 (MQ=255)
                                                                                                                                           aaCCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGcc                          <  1:9931/251‑1 (MQ=255)
                                                                                                                                                gTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCggcc                     >  1:1070/1‑248 (MQ=255)
                                                                                                                                                                   aGCTGAAAGCCAGACGTAACAGCACCACGGTGGCGGTGCACACGGTGGGCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTAACGTGTCATCCTGCAGGCTGACGGCTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgctgcgtcgtctt  >  1:150431/1‑237 (MQ=255)
                                                                                                                                                                     ntGAAAGCCAGACGTAACAGCACCACGGTGGTNGNGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATNGATGTGGAGTNCGGNNAGTANANTNTCATCCTGCAGGTTGACGGTTTTCCACCANCGCACGCCGGGACCATCACCGTGNNTGNNNATNNACAAc                                                                         <  2:121871/177‑1 (MQ=255)
                                                                                                                                                                     cTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCAGGATGAAGACGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGAAGGATGACGGTTTTCCACCATCGCACGACGGGACCATCACCGTGTATGAAGATTCACAAc                                                                         >  1:121871/1‑178 (MQ=255)
                                                                                                                                                                                                               |                                                                                                                                                                                                              
GTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTT  >  NC_000913/1428953‑1429366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑