Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,423,244	0	G	C	50.0%	8.8 / 16.9	14	V165L (GTG→CTG)	ydaW	putative uncharacterized protein YdaW
Reads supporting (aligned to +/- strand):  ref base G (3/4);  new base C (3/4);  total (6/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTGCGATTTAACCGCGACGATTGAACAACGAGGACCACAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCGTTACTGCATACCGGGCGATAATTTACCAGCAGAGCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGGGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGAGATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGC  >  NC_000913/1422994‑1423486                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   gTGCGATTTAACCGCGACGATTGAACAACGAGGACCACAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCGTTACTGCATACCGGGCGATAATTTACCAGCAGAGCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATgggg                                                                                                                                                                                                                                                    <  1:128765/251‑1 (MQ=255)                       gAACAACGAGGACCACAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCGTTACTGCATACCGGGCGATAATTTACCAGCAGAGCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGGGGTGTTAATCATTACCGTGATAAc                                                                                                                                                                                                                              >  1:57800/1‑251 (MQ=255)                                               gatgaGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCGTTACTGCATACCGGGCGATAATTTACCAGCAGAGCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGGGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAgcgc                                                                                                                                                                                                      <  1:37754/251‑1 (MQ=255)                                                                                                                                                                   cATACCGGGCGATAATTTACCAGCAGAGCCGAAAGCAGAATCGGTATCTCAGCTATGGTTATCTGCATCGTCGTCTGTCTCTCATGGGGTGTTCCTCATTACCGTGATAACGCCATCGCCTCCACAGAACAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGc                                                                                                                                                                         >  1:159021/1‑164 (MQ=37)                                                                                                                                                                      aCCGGGCGATAATTTACCAGCAGAGCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGGGGTGTTAATCATTCCCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACAATAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGAGTTGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATTCAAAAGACACGAAGGAGTTAGCTGAGGCGAAAg                                                                                <  1:54888/250‑1 (MQ=255)                                                                                                                                                                                                    agcagcATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGGGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGAGATGCAGATGCGTCAGCGTTATGTTGCTGCGCTCGAGGCAAAATACACGAAGGAGTTAGCGGATGCGAAAGCTGAAAATGATGCTCTGCGTGATGATGttcc                                                 <  1:160278/251‑3 (MQ=255)                                                                                                                                                                                                        gcaTCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGGGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGAGATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGcn                                             <  1:103186/251‑2 (MQ=255)                                                                                                                                                                                                               tNNCTCCGCNNTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTCCCGTGATAACGCCACTACCTACAAAGCCCAGCGCGACaaaaa                                                                                                                                                                                              <  2:8790/99‑1 (MQ=14)                                                                                                                                                                                                                  ctcCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTc                                                                                                                     <  1:51657/169‑1 (MQ=11)                                                                                                                                                                                                                    ccGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACTCGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGgtcgtcgtc                                   <  1:54199/249‑1 (MQ=11)                                                                                                                                                                                                                                        atcgtnNGCNNNTCNNGGGCTGTTAATCATTACCGTGATNACGCCATTACCTACAAAGCCCAGCGCGACNANANTGCCNNAGANCTGAAGCTGGNGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCNCNCGATGCAACATACACGCCGGAGTTAGCTGAGNCTAAAGCTGACCATGATGCTCTGcnggagnnggnt                                                   >  2:127479/2‑202 (MQ=11)                                                                                                                                                                                                                                        atcgtcTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTgg                                           <  1:127479/220‑1 (MQ=11)                                                                                                                                                                                                                                          cgtcTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGCTGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTgtc           >  1:11756/1‑251 (MQ=11)                                                                                                                                                                                                                                                 cTGTCATGGGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGAGATGCAGATGCGTCAGAGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGCTGCGAAAGCTGAAAATGATGCTCTGCGtg                                                           >  1:161316/1‑196 (MQ=255)                                                                                                                                                                                                                                                   ntCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATATTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGc  >  1:30814/2‑251 (MQ=11)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   GTGCGATTTAACCGCGACGATTGAACAACGAGGACCACAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCGTTACTGCATACCGGGCGATAATTTACCAGCAGAGCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGGGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGAGATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGC  >  NC_000913/1422994‑1423486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑