Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 2,269,876 IS5 (+) +4 bp intergenic (+309/‑100) lpxT → / → mepS lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2269876 =0 (0.000)9 (1.100) 7/332 0.1 100% intergenic (+309/‑103) lpxT/mepS lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
?NC_000913 2288919 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 22698790 (0.000)9 (1.100) 6/334 0.2 100% intergenic (+312/‑100) lpxT/mepS lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region

CTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCAGTTTTTAAAAACTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATG  >  NC_000913/2289980‑2290358
                                                                                                                                     |                                                                                                                                                                                                                                                     
ctctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGCAAGTTGATGACACATGATGAACCCTGATCTATGGCTCCAGCTGCCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACa                                                                                                                                                                                                     >  1:148656/1‑184 (MQ=18)
         ggTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAcc                                                                                                                           <  1:63714/249‑1 (MQ=255)
                  gcgcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGACCCCTGTTCTAGGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGTTGTAGCCACCAGAACAGATATTGCGGAACGATAAAGAGAAACAGAACCAGGTTGATGCATTGAGCTTACAGCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTaaa                                                                                                                                    <  1:159503/231‑3 (MQ=25)
                                                                                                                                ccTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCAGTTTTTAAAAACTGGTTAAAGAAATGCACAAGTAGTGTGATTGATTGGTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATg  <  1:107792/251‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                                                                                                                                     
CTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCAGTTTTTAAAAACTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATG  >  NC_000913/2289980‑2290358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.