Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,740,265	G→A	R187R (CGG→CGA)	avtA →	valine‑‑pyruvate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,740,265	0	G	A	80.0%	19.2 / 2.9	15	R187R (CGG→CGA)	avtA	valine‑‑pyruvate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (4/8);  total (6/9)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.18e-01

GGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCACCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTGAGCATCTGCATATTGGCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGGCTCCCGCTGCGGCATTATCATCGCCAATGA  >  NC_000913/3740059‑3740495                                                                                                                                                                                                               |                                                                                                                                                                                                                                       ggACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCACCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGATTGTCTCTGCGCTTCCGAATACTGAACTGCTGCCGGAAGGCCAGTGTAAATACCACGGCGATTTTGAGCATGGGCAGATTGGCGAAGAAACCTGGATGAGTTGCGTCTCCCGACCTTCGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCt                                                                                                                                                                                            <  1:188804/251‑1 (MQ=255)          ggcGATGGTCGGGTCAAAAA‑GTGCTGTTCCCGCTTGCACCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTGAGCATCTGCATATTGGCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAagag                                                                                                                                                                                                 >  1:299577/3‑236 (MQ=255)                                       cccGCTTGCACCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCGGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTGAGCATCTGCATATTGGCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGAGTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTcc                                                                                                                                                        <  1:120679/249‑1 (MQ=255)                                                                   gCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTGAGCATCTGCATATTGGCGAAGAAACCGGGATGATTTGCGTCTCCCGAGCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTGGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTcc                                                                                                                           <  1:302681/250‑1 (MQ=255)                                                                                                                        aCTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTGAGCATCTGCATATTGGCGAAGAAACCGGGATGATTTGCGTCTCCCGACCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAATCGCGCCCGCTATGGAATCCGAATATCg                                                                     <  1:249147/251‑1 (MQ=255)                                                                                                                                                          naTTTTGAGCATCTGCATATTGGCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCa                                                            >  1:195437/2‑226 (MQ=255)                                                                                                                                                                gAGCATCTGCATATTGGCGAAGAAACCGGGATGATTTGCGTCTCCCGACCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCGTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGGCtn                             <  1:31423/251‑2 (MQ=255)                                                                                                                                                                        ncNTATTGGCGAAGAAACCGGGATGATTTGCGTCTCCCNACCNNCGNNTCNAACAg                                                                                                                                                                                                                        <  2:177302/55‑1 (MQ=255)                                                                                                                                                                        gCATATTGGCGAAGAAACCGGGATGATTTGCGTCTCCCGACCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCTAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGGCTCCCGc                         <  1:237242/247‑1 (MQ=255)                                                                                                                                                                                  naagaaACCGGGATGATTTGCGTCTCCCGACCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                                                                                                   >  1:204151/2‑131 (MQ=255)                                                                                                                                                                                  gaagaaACCGGGATGATTTGCGTCTCCCGACCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                                                                                                   <  2:204151/131‑1 (MQ=255)                                                                                                                                                                                            ggATGATTTGCGTCTCCCGACCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGGCTcc                            <  1:13103/224‑1 (MQ=255)                                                                                                                                                                                            ggATGATTTGCGTCTCCCGACCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGATGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTCCCTGGCTCCCGCTGCGGCATTATCATCGCCAATGa  >  1:279562/1‑250 (MQ=255)                                                                                                                                                                                                 atTTGCGTNNCCNNANANACGAANACAACAGGCAATGTGATTACTGACGAAGAGTTGc                                                                                                                                                                                             >  2:214054/1‑58 (MQ=255)                                                                                                                                                                                                      ngTCTCCCGACCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAg                                              >  1:68546/2‑196 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                                       GGACGCCGTGCCGATGGTCGGGTCAAAAAAGTGCTGTTCCCGCTTGCACCGGAATACATTGGCTATGCTGACGCCGGACTGGAAGAAGATCTGTTTGTCTCTGCGCGTCCGAATATTGAACTGCTGCCGGAAGGCCAGTTTAAATACCACGTCGATTTTGAGCATCTGCATATTGGCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAATCCGAATATCGTGCTGTGCATGAGTCTTTCCAAGCTGGGTCTACCTGGCTCCCGCTGCGGCATTATCATCGCCAATGA  >  NC_000913/3740059‑3740495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑