Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,711,620	G→A	P130S (CCG→TCG)	yhjX ←	putative transporter YhjX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,711,620	0	G	A	91.7%	27.5 / ‑6.0	12	P130S (CCG→TCG)	yhjX	putative transporter YhjX
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (5/6);  total (6/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCGTCTTTCATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTCCCCAAATCACAAAGGTTTTTTCCAGACCGACCGTTTCCAGCAGCTGCGTGTCGATAAATTTGAAACCCAGGCTACCCAGACCATAAGAACCGATAGCGAACGCGGAGATCAGACCTTTACGCTCCGGGAACCACTTCACACAGTTAGAGAGCGTCAGCAGATAACCCGCGCCATCTGCCAGTCCCACCAGCACACCGGCGCTTAACCACAGCATCATCAGGTTGTCAGAATGCGCTGTCAGGAAGAAGCCTAATCCCAGCAAAATGCCGGAAGCCATGGTGACGCGTTTAACGCCAAAACGTTCCTGTAATTTGCCCGCAACAGAAGACGAAATTGCCAGCCCCAGACTTAAC  >  NC_000913/3711440‑3711846                                                                                                                                                                                     |                                                                                                                                                                                                                                   gTGCGTCTTTCATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTCCCCAAATCCCAAAGGTTTTTTCCAGACCGACCGTCTCCAGCAGCTGCGTGTCGATAAATTTGAAACCCAGGCTCCCCCGACCATAAGAACCGCTAGCGAACGCGGAGATCAGACCTTTACGCTCCGAGAACCACTTCACACAGTTag                                                                                                                                                                                                                >  1:230892/1‑201 (MQ=255)                              aCAATCATCAACAGCGCAATCGCTCCCCAAATCACAAAGGTTTTTTCCAGACCGACCGTTTCCAGCAGCTGCGTGTCGATAAATTTGAAACCCAGGCTACCCAGACCATAAGAACCGATAGCGAACGCGGAGATCAGACCTTTACGCTCCGAGAAc                                                                                                                                                                                                                                <  1:298210/156‑1 (MQ=255)                                              cAATCACTCCCCAAATCACAAAGGATTTTTCCAGACCGACCGTTTCCAGCCGGTGCGTGTCGCTAAATTTGAAACCCCGGCTACCCAGACCATCAGCACCGATAGCGAACGCGGAGATCAGACCTTTACGCTCCGGGAACCACCACACACAGTTAGAGAGCGTCAGCAGATAAccc                                                                                                                                                                                            >  1:56934/1‑176 (MQ=255)                                                        ccAAATCACAAAGGTTTTTTCCAGACCGACCGTTTCCAGCAGCTGCGTGTCGATAAATTTGAAACCCAGGCTACCCAGACCATAAGAACCGATAGCGAACGCGGAGATCAGACCTTTACGCTCCGAGAACCACTTCACACAGTTAGAGAGCGTCAGCGGAt                                                                                                                                                                                                 >  1:132057/1‑161 (MQ=255)                                                         caagtCACAAAGGTTTTTTCCAGACCGACCGTTTCCAGCAGCTGCGTGTCGATAAATTTGAAACCCAGGCTACCCAGACCATAAGAACCGATAGCGAACGCGGAGATCAGACCTTTACGCTCCGAGAACCACTTCACACAGt                                                                                                                                                                                                                   <  1:11664/138‑1 (MQ=255)                                                                                                  cTGCGTGTCGATAAATTTGAAACCCAGGCTACCCAGACCATAAGAACCGATAGCGAACGCGGAGATCAGACCTTTACGCTCCGAGAACCACTTCACACAGTTAGAGAGCGTCAGCAGATAACCCTCTCCATCTGc                                                                                                                                                                                 <  1:270883/135‑1 (MQ=255)                                                                                                          cGATAAATTTGAAACC‑AGGCTACCCAGACCATAAGAACCGATAGCGAACGCGGAGATCAGACCTTTACGCTCCGAGAACCACTTCACACAGTTAGAGAGCGTCAGCAGATAACCCGCGCCATCTGCCAGTccc                                                                                                                                                                          >  1:263236/1‑133 (MQ=255)                                                                                                                       aCCCAGGCTACCCAGACCATAAGAACCGATAGCGAACGCGGAGATCAGACCTTTACGCTCCGAGAACCACTTCACACAGTTAGAGAGCGTCAGCAGATAACCCGCGCCATCTGCCAGTCCCACCAGCACACCGGCGCGTAACCACAGCATCATCAGGTTGTCAGAATGCGCTGTCAGGAAGAAGCCTAATCCCAGCAAAATGCCGGAAGCCAGGGTGACGCGTTTAACGCCAAAACGTTCCTGTAATTTGc                                        <  1:236496/251‑1 (MQ=255)                                                                                                                                       ccATAAGAACCGATAGCGAACGCGGAGATCAGACCTTTACGCTCCGAGAACCACTTCACACAGTTAGAGAGCGTCAGCAGATAACCCGCGCCATCTGCCAGTCCCACCAGCACACCGGCGCTTAACCACAGCATCATCAGGTTGTCAGAATGCGCTGTCAGGAAGAAGCCTAATCCCAGCAAAATGCCGGAAGCCATGGTGACGCGTTTAACGCCAAAACGTTCCTGTAATTTGCCCGCAACAGAAGACg                         >  1:110741/1‑250 (MQ=255)                                                                                                                                                         aaNGCGGANATCANACCTTTACGCTCCGANAACCACTTCACACAGTTAGNNANCGTCANCGGAt                                                                                                                                                                                                 <  2:132057/64‑1 (MQ=255)                                                                                                                                                             cgGAGATCAGACCTTTACGCTCCGAGAACCACTTCACACAGTTAGAGAGCGTCAGCAGATAACCCGCGCCATCTGCCAGTCCCACCAGCACACCGGCGCTTAACCACAGCATCATCAGGTTGTCAGAATGCGCTGTCAGGAAGAAGCCTAATCCCAGCAAAATGCCGGAAGCCATGGTGACGCGTTTAACGCCAAAACGTTCCTGTAATTTGCCCGCAACAGACGACGAACTTGCCAGCCCCAGACTTAAc  >  1:238638/1‑251 (MQ=255)                                                                                                                                                                                cTCCGAGAACCACTTCACACAGTGAGAGAGCGTCAGCAGATAACCCGCGCCATCTGCCAGTCCCACCAGCACCCCGGCGCTTAACCACAGCATCATCAGGTTGTCAGAATGCGCTGTCAGGAAGAAGCCTAATcc                                                                                                   <  1:251572/135‑1 (MQ=255)                                                                                                                                                                                     |                                                                                                                                                                                                                                   GTGCGTCTTTCATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTCCCCAAATCACAAAGGTTTTTTCCAGACCGACCGTTTCCAGCAGCTGCGTGTCGATAAATTTGAAACCCAGGCTACCCAGACCATAAGAACCGATAGCGAACGCGGAGATCAGACCTTTACGCTCCGGGAACCACTTCACACAGTTAGAGAGCGTCAGCAGATAACCCGCGCCATCTGCCAGTCCCACCAGCACACCGGCGCTTAACCACAGCATCATCAGGTTGTCAGAATGCGCTGTCAGGAAGAAGCCTAATCCCAGCAAAATGCCGGAAGCCATGGTGACGCGTTTAACGCCAAAACGTTCCTGTAATTTGCCCGCAACAGAAGACGAAATTGCCAGCCCCAGACTTAAC  >  NC_000913/3711440‑3711846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑