Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	466,049	C→T	A422T (GCA→ACA)	ybaE ←	uncharacterized protein YbaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	466,049	0	C	T	83.3%	21.7 / ‑2.5	12	A422T (GCA→ACA)	ybaE	uncharacterized protein YbaE
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (4/6);  total (6/6)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAGCGTCGAAAACATGTGGCCACAGCGGATCGCAGCGCAGCCATTGCTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCT  >  NC_000913/465846‑466243                                                                                                                                                                                                            |                                                                                                                                                                                                   tGGAGCGTCGAAAACATGTGGCCACAGCGGATCGCAGCGCAGCCATTGCTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATTATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGTCATGGTATGAAg                                                                                                                                                                                        <  1:242949/216‑1 (MQ=255)       gTCGAAAACATGTGGCCACAGCGGATCGCAGCGCAGCCATTGCTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGt                                                                                                                                                >  1:106492/1‑250 (MQ=255)            aaaCATGTGGCCACAGCTGATCGCAGCGCAGCCATTGCTCCAGATTATATTCCGGTGCTTCGCCAATTATTCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTGCACAGCCTTCCGCTGCCAGTGTCGCCTTTATGCGGTCTGTCATCGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTg                                                                                                                                          <  1:121599/251‑1 (MQ=255)                                                 cTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGTCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAcc                                                                                                                           >  1:129983/1‑229 (MQ=255)                                                                         gCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGTCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTg                                                                                                                                          <  1:11193/190‑1 (MQ=255)                                                                                                                                     gTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGTCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGt                                                                                                                                             <  1:148738/127‑1 (MQ=255)                                                                                                                                             ngTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTCTGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGATTGTAATACACCGGATTGATGAATAATGGAGATCACtttt         >  1:58652/2‑251 (MQ=255)                                                                                                                                             cGTTATGAAAAATAATTGTGAGTTCACAGCCTTCAGCTGCCAGTGTCGCCTGTAGGCGTTCTGTCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTATCCGACTTCTAAAGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACtttt         >  1:278783/1‑251 (MQ=255)                                                                                                                                              gTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGTCAGGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGGCAAGGTTTTCGGTAGTTTGACTTCATCCGTTACCTGCCAATGCGTAATAGTCCAGCCAGGCAg                                                                                              <  1:93427/165‑1 (MQ=255)                                                                                                                                                    aaaaaTAATTGTGAGTTCACAGCCTTCCGCTGCAAGTGTCGCCTGTAGGCGTTCTGTAATGGTATGAAGATCTATAGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAACTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGGGATCATGTTTTCTCCGACTTCTAACGTTTTTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCt  >  1:216779/1‑251 (MQ=255)                                                                                                                                                                          cgttCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGTCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTAAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAAt                                                                                                                    >  1:295459/3‑115 (MQ=255)                                                                                                                                                                                   gCCAGTGTCGCCTGGAGGCGTTCTGTCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGGCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCAAACGTTTGTAATAAACCGGATTGATGAATAATg                     <  1:303041/201‑1 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                                   TGGAGCGTCGAAAACATGTGGCCACAGCGGATCGCAGCGCAGCCATTGCTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCT  >  NC_000913/465846‑466243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑