Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,614,434	G→A	Q256Q (CAG→CAA)	nikA →	Ni(2(+)) ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,614,434	0	G	A	92.3%	25.6 / ‑5.7	13	Q256Q (CAG→CAA)	nikA	Ni(2(+)) ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (10/2);  total (11/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGACTACCCGCGCGGTGGCGTTTGAAACTGGCGATATCGACCTGCTGTACGGAAACGAAGGGTTATTACCGCTCGATACCTTCGCCCGCTTTAGCCAGAATCCGGCTTACCACACCCAACTGTCACAGCCGATCGAAACCGTGATGCTGGCGCTCAATACCGCCAAAGCCCCCACCAACGAGCTGGCAGTACGTGAAGCTCTTAATTACGCGGTAAACAAAAAATCGCTGATTGATAACGCGTTGTATGGCACCCAGCAGGTCGCCGACACCCTGTTTGCCCCTTCTGTGCCCTACGCCAACCTCGGCCTGAAACCGAGCCAGTACGATCCGCAAA  >  NC_000913/3614289‑3614672                                                                                                                                                  |                                                                                                                                                                                                                                               gCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCCTCCCGGACCCGACTACCCGCGCGGTGGCGTTTGAAACTGGCGCTATCGACCTGCTGTACGGAAACGAAGGGTTATTACCGCTCGATACCTTCGCCCGCTTTAGCCAACATCCGGCTTACCACACCCAACTGTCACAGCCGATCGAAACCGTGATGCTGGCGCTCAATACCGCCAAAGCCCcca                                                                                                                                                                    >  1:282386/1‑222 (MQ=255)  cGAAAAGACAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGACTACCCGCGCGGTGGCGTTTGAAACTGGCGATATCGACCTGCTGTACGGAAACGAAGGGTTATTACCGCTCGATACCTTCGCCCGCTTTAGCCAAAATCCGGCTTACCACACCCAACTGTCACAGCCGATCGAAAcc                                                                                                                                                                                                      >  1:271381/1‑187 (MQ=255)                                              ncccGACTACCCGCGCGGTGGCGTTTGAAACTGGCGATATCGACCTGCTGTACGGAAACGAAGGGTTATTACCGCTCGATACCTTCGCCCGCTTTAGCCAAAATCCGGCTTACCACACCCAACTGTCACAGCCGATCGAAACCGTGATGCTGGCGCTCAATACCGCCAAAGCCCCCACCAACGAGCTGGCAGTACGTGAAGCTCTTAATTACGCGGTAAACAAAAAATCGCTGATTGATAACGCGTTGTAt                                                                                          >  1:78656/2‑251 (MQ=255)                                               cccGACTACCCGCGCGGTGGCGTTTGAAACTGGCGATATCGACCTGCTGTACGGAAACGAAGGGTTATTACCGCTCGATACCTTCGCCCGCTTTAGCCAAAATCCGGCTTACCACACCCAACTGTCACAGCCGATCGAAACCGTGATGCTGGCGCTCAATACCGCCAAAGCCCCCACCAACGAGCTGGCAGTACGTGAAGCTCTTAATTACGCGGTAAACAAAAAATCGCTGATTGATAACGCGTTGTATg                                                                                         >  1:170190/1‑251 (MQ=255)                                                                         aaaCTGGCGATATCGACCTGCTGTACGGAAACGCAGGGTTATTACCGCTCGATACCTTCGCCCGCTTTACCCAGAATCTGGCTTAAAACACCCAACTGTCACAGCCGATCGAAACCGTGATGCTGGCGATCAATACCGCCAAAGCCCCCACCAACGAGCTGGc                                                                                                                                                       >  1:292612/1‑163 (MQ=255)                                                                         aaaCTGGCGATATCGACCTGCTGTACGGAAACGAAGGGTTATTACCGCTCGATACCTTCGCCCGCTTTAGCCAAAATCCGGCTTACCACACCCAACTGTCACAGCCGATCGAAACCGTGATGCTGGCGCTCAATACCGCCAACGCCCCCACCAACGAGCTGGCAGTACGTGAAGCTCtt                                                                                                                                       >  1:1266/1‑179 (MQ=255)                                                                                           tgctgTACGGAAACGAAGGGTTATTACCGCTCGATACCTTCGCCCGCTTTAGCCAAAATCCGGCTTACCACACCCAACTGTCACAGCCGATCGAAACCGTGATGCTGGCGCTCAATACCGCCAAAGCCCCCACCAACGAGCTGGc                                                                                                                                                       <  1:168240/145‑1 (MQ=255)                                                                                               gTACGGAAACGAAGGGTTATTACCGCTCGATACCTTCGCCCGCTTTAGCCAAAATCCGGCTTACCACACCCAACTGTCACAGCCGATCg                                                                                                                                                                                                           >  1:47659/1‑89 (MQ=255)                                                                                                    gAAACGAAGGGTTATTACCGCTCGATACCTTCGCCCGCTTTAGCCAAAATCCGGCTTACCACACCCAACTGTCACAGCCGATCg                                                                                                                                                                                                           <  1:2016/84‑1 (MQ=255)                                                                                                    gAAACGAAGGGTTATTACCGCTCGATACCTTCGCCCGCTTTAGCCAAAATCCGGCTTACCACACCCAACTGTCACAGCCGATCg                                                                                                                                                                                                           >  2:2016/1‑84 (MQ=255)                                                                                                                               ccTTCGCCCGCTTTAGCCAAAATCCGGCTTACCACACCCAACTGTCACAGCCGATCGAAACCGTGATGCTGGCGCTCAATACCGCCAAAGCCCccacc                                                                                                                                                                  >  1:71533/1‑98 (MQ=255)                                                                                                                                nttCGCCCGCTTTAGCCAAAATCCGGCTTACCACACCCAACTGTCACAGCAGATCGAAACCGTGATGCTGGCGCTCAATACCGCCAAAGCCCCCACCAACGAGCTGGCAGTACGTGAAGCTCTTAATTACGCGGTAAACAAAAAATCGCTGATTGATAACGCGTTGTa                                                                                           >  1:65363/2‑168 (MQ=255)                                                                                                                                       cGCTTTAGCCAAAATCCGGCTTACCACACCCAACTGTCACAGCCGATCGAAACCGTGATGCTGGCGCTCAATCCCGCCAAAGCCCCCACCAACGAGCTGGCAGTACGTGAAGCTCTTAATTACGCGGTAAACAAAAAATCGCTGATTGATAACGCGTTGTATGGCACCCAGCAGGTCGCCGACACCCTGTTTGCCCCTTCTGTGCCCTACGCCAACCTCGGCCTGAAACCGAGCCAGTACGATCCGCaaa  >  1:271665/1‑250 (MQ=255)                                                                                                                                                  |                                                                                                                                                                                                                                               GCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGACTACCCGCGCGGTGGCGTTTGAAACTGGCGATATCGACCTGCTGTACGGAAACGAAGGGTTATTACCGCTCGATACCTTCGCCCGCTTTAGCCAGAATCCGGCTTACCACACCCAACTGTCACAGCCGATCGAAACCGTGATGCTGGCGCTCAATACCGCCAAAGCCCCCACCAACGAGCTGGCAGTACGTGAAGCTCTTAATTACGCGGTAAACAAAAAATCGCTGATTGATAACGCGTTGTATGGCACCCAGCAGGTCGCCGACACCCTGTTTGCCCCTTCTGTGCCCTACGCCAACCTCGGCCTGAAACCGAGCCAGTACGATCCGCAAA  >  NC_000913/3614289‑3614672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑