Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,572,984 G→A G173G (GGC→GGT)  glgB ← 1,4‑alpha‑glucan branching enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,572,9840GA81.8% 14.7 / ‑2.3 12G173G (GGC→GGT) glgB1,4‑alpha‑glucan branching enzyme
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (2/7);  total (3/9)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

CCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCATTTGCGCTTCAAAGGCATAAGGGTCGGACTTCAGACGCAAGTTGCCATTGGCATCAATCATCTCGTATTTATAGAGCTGACCGTTATGCGCCCCAGGGATAAACAGTTCCCAGATGCCGCTCTCTTTACGCAGGCGCATCGGGTGACGGCGACCGTCCCAGTAGTTGAATTGCCCAACCACCGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGT  >  NC_000913/3572744‑3573225
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 
ccAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCATTTGCGCTTCAAAGGCATAAGGGTCGGATTTCAGACGCAAGTTGCCATTGGCATCAATCATCTCGTATTTATAGAGCTGACCGTTATGCGCCCCAGGGATAAACAGTTCCCAGATACCGCTCTCtt                                                                                                                                                                                                                                         >  1:77354/1‑251 (MQ=255)
       cccAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCATTTGCGCTTCAAAGGCATAAGGGTCGGACTTCAGACGCAAGTTGCCATTGGCATCAATCATCTCGTATTTATAGAGCTGACCGTTATGCGCCC‑AGGGATAAACAGTTCCCAGATGCCGCTCTCTTTACGCAgg                                                                                                                                                                                                                                 >  1:220717/1‑251 (MQ=255)
                                                             cgcTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCATTTGCGCTTCAAAGGCATAAGGGTCGGACTTCAGACGCAAGTTGCCATTGGCATCAAGCATCTCGTAGTTATAGAGCTGACCGTTATGCGCCCCAGGGATAAACAGTTCCCAGATACCGCGCTCTTTACGCAGGCGGATCGGGTTACGGCGACCGTCCCAGTAGTTGAATTGCCCAACCACCgagan                                                                                                                                                                            <  1:63747/251‑2 (MQ=255)
                                                                                                             gcgGTTTCCGGGCGCATTTGCGCTTCAAAGGCATAAGGGTCGGACTTCAGACGCAAGTTGCCATTGGCATCAAGCATCTCGTATTGATAGAGCTGACCGTTATGCGCCCCAGGGATAAACAGTTCCCAGATACCGCTCTCTTTACGCAGGCGCATCGGGTGAc                                                                                                                                                                                                                    <  1:119498/163‑1 (MQ=255)
                                                                                                                                                     cGGACTTCAGACGCAAGTTGCCAGTGGCATCAATCATCTCGGATTTATAGAGCTGACCGTTATGCGCCCCAGGGATAAACAGTTCCCAGATACCGCTCTCTTTACGCAGGCGCATCGGGTGACGGCGACCGTCCCAGTAGTTGAATTGCCCAACCCCCGAGACCCGACGGGCGTTTGGAGCCCAGCCAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGGTTCATACggn                                                                                    <  1:82172/251‑2 (MQ=255)
                                                                                                                                                       gACTTCAGACGCAAGTTGCCATTGGCATCAATCATCTCGTATTTATAGAGCTGACCGTGATGCGCCCCAGGGATAAACAGTTCCCAGATACCGctctc                                                                                                                                                                                                                                           <  1:318408/98‑1 (MQ=255)
                                                                                                                                                        atntctnannanngTTNCCATTGGCATCTATCATCGCTNNTTTATAGAGNTGATCATGATCCGCCCCAGGGATAAACAGTTCTCAGAGCCCACTCTCCTTACGNCGTCGCATCNNGTNACGGCNANNNGNNNAGNAGGTGAATTGCCCAACCACNGANNCCCGACNGGCGTTTGGAGCAATGACAGAGAAAc                                                                                                                                            <  2:77354/179‑1 (MQ=255)
                                                                                                                                                                  cAAGTTGCCATTGGCAGCAATCATCTCGTAGTTATAGAGCTGACCGGTATGCGCCCCAGGGATAAACAGTTCCCAGATGCCGCTCTCTTTACGCAgg                                                                                                                                                                                                                                 <  1:290385/97‑1 (MQ=255)
                                                                                                                                                                                                               gTTATGCGCCCCAGGGATAAACAGTTCCCAGATACCGCTCTCTTTACGCAGGCGCATCGGGTGACGGCGACCGTCCCAGTAGTTGAATTGCCCAACCACCGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACg                                                                                      <  1:110032/191‑1 (MQ=255)
                                                                                                                                                                                                                       ccccAGGGATAAACAGTTCCCAGATACCGCTCTCTTTACGCAGGCGCATCGGGTGACGGCGACCGTCCCAGTCGTTCAATTGCCCAACCACCGAGACCCGCCGGGCGTTTGGAGCCCAGACAGAGACACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTa                   >  1:159569/1‑250 (MQ=255)
                                                                                                                                                                                                                                        tCCCAGATACCGCTCTCTTTACGCAGGCGCATCGGGTGACGGCGACCGTCCCAGTAGTTGAATTGCCCAACCACCGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCATACCTGTGACGCCATCCATAGTATCTGCATGCGCGTCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATTATAGCCAGTCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGt  <  1:178276/250‑1 (MQ=255)
                                                                                                                                                                                                                                           cAGATACCGCTCTCTTTACGCAGGCGCATCGGGTGACGGCGACCGTCCCAGTAGTTGAATTGCCCAACCACCGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGAc                                                                                                                               <  1:100116/122‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 
CCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCATTTGCGCTTCAAAGGCATAAGGGTCGGACTTCAGACGCAAGTTGCCATTGGCATCAATCATCTCGTATTTATAGAGCTGACCGTTATGCGCCCCAGGGATAAACAGTTCCCAGATGCCGCTCTCTTTACGCAGGCGCATCGGGTGACGGCGACCGTCCCAGTAGTTGAATTGCCCAACCACCGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGT  >  NC_000913/3572744‑3573225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑