Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	384,127	C→T	V164I (GTT→ATT)	yaiS ←	putative deacetylase YaiS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	384,127	0	C	T	90.9%	19.1 / NA	11	V164I (GTT→ATT)	yaiS	putative deacetylase YaiS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (4/6);  minor base G (0/1);  total (4/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

AAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGCGGAAATCATATCATTGAGCTGTAAATGAGCGCGGG  >  NC_000913/383885‑384351                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 aaaCCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGGCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAATCTGAGGcn                                                                                                                                                                                                                          <  1:95632/251‑2 (MQ=255)                                                            cTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGCCCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGAGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAATCTGAGGCATAAATGCAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCggc                                                                                                                                                                 >  1:156465/1‑249 (MQ=255)                                                                                                       gCTATTGACCTGTTGTCCGCGAAAGTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTGTTATGTTTTTTTAATGCAGCAAGTTTGACGGTGAAATATTCTTCTGTAACGGAGTCAAAAAGCTGAGGCATAAATTAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAAGTTGCAGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGc                                                                                                                    <  1:311662/251‑1 (MQ=255)                                                                                                                     gtCCGCGAAATTGCGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAATCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCTGAATAGTTCTGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATcatg                                                                                                       <  1:224185/250‑1 (MQ=255)                                                                                                                                   tcAACTGCACGCAGTCGATCGTGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTGGACTGGGAAATATTCTTCTTTAACGGATTCAAAAATCTGAGGCATAAATGAAATCCAgg                                                                                                                                                                                                           <  1:269877/135‑1 (MQ=255)                                                                                                                                                   gNTCATGACGCATATAATCGCGTAGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAATCTGAGGCATAAATGAAAGCCa                                                                                                                                                                                                             >  1:253499/1‑118 (MQ=255)                                                                                                                                                                   aTCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAATCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCAGGCATGGTATATACCCTCATGAt                                                                                  <  1:114264/225‑1 (MQ=255)                                                                                                                                                                                                     gnagcaAGTTTGACTGTGAAATANNCTTCTTTAANGGAGTCAAAAATCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCNGAGAAGGTGNNGANTAGTGNGNNNGNNNACNATTGAAGCTTGATAAACAGNAANNTGATCCNGATGGCGGTCGGCATCATGCATGGta                                                                                                <  2:77004/177‑1 (MQ=255)                                                                                                                                                                                                     gcagcaAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAATCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGta                                                                                                >  1:77004/1‑177 (MQ=255)                                                                                                                                                                                                        gcaAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAATCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGCGGAAatc                              >  1:243507/1‑240 (MQ=255)                                                                                                                                                                                                                         atatTCTTCTTTAACGGATTCAAAAATCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCAGGGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAAGTTGATTTTTAATGATGTCTTCCAGGGCGGAAATCATATCATTGAGCTGTAAATGAGCGCggg  <  1:308062/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 AAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGCGGAAATCATATCATTGAGCTGTAAATGAGCGCGGG  >  NC_000913/383885‑384351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑