Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,268,970	A→G	T186A (ACA→GCA)	yhaC →	uncharacterized protein YhaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,268,970	0	A	G	100.0%	38.7 / NA	14	T186A (ACA→GCA)	yhaC	uncharacterized protein YhaC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/9);  total (5/9)

CATTAATGAACCGTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCG  >  NC_000913/3268738‑3269117                                                                                                                                                                                                                                         |                                                                                                                                                    naTTAATGAACCGTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCGAATTTTACTAATGcc                                                                                                                                    >  1:60774/2‑250 (MQ=255)             gTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGAGAGCGTTGATCTATCCTCTCTGTACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAAGTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAgg                                                                                                                       <  1:182733/251‑1 (MQ=255)                aaaaaaCCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCTCAGATAAACATATTGCTAATACTATTTTAAATCCTTTTAAATGGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGtt                                                                                                                     <  1:320906/250‑1 (MQ=255)                                      ggTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAATGTAATTTTTAGTTGGGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGGTAATTCTATTTTAAAGCCTTTTAAATTGAATTTAGCAAATTTTACTAATGCTAACATGTTGCAGGTTAATTTTGTTAGTTCAacac                                                                                                  <  1:294415/247‑1 (MQ=255)                                                          cccATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGATGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGatt                                                                          >  1:116400/1‑251 (MQ=255)                                                             aTTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCGAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTGGTAAGTGCAACACAAAAc                                                                                             <  1:110555/229‑1 (MQ=255)                                                                                                            gTTGTGAGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGCTTATTATGATTTCACAGATAAACATATGGCTAATACGATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACAAAAAACGCcac                                                                                        >  1:220100/1‑187 (MQ=255)                                                                                                                      tGGAGATATCGTTGATCTATCCTCTCTGGACTTACGGAATTTCGAGTTAGATTATTATGAGATCACAGATAAACATATGGCTAATTCTATTTTAAATCCTTTTAAATTTAATTCAGCAAATTTTACGAAGGCTAACAGGTTTCAGGTTAATTTGGTTAGTTCAAcaca                                                                                                 <  1:137102/168‑1 (MQ=255)                                                                                                                       ggAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAGTTGAATTCAGCAAATTTTACTAATGCCANCATNNNNNAg                                                                                                                        >  2:198168/1‑144 (MQ=255)                                                                                                                               cGTTGATCTATCCTCTCTGGACTGACGTAATGTCTATTTATATTATTATGATTTCTGAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGtn                                                                                                                            <  1:39118/132‑2 (MQ=255)                                                                                                                                   gATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAGTTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTcn  <  1:198168/250‑2 (MQ=255)                                                                                                                                                                    ttAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACAGGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTgn                                                                              <  1:64943/141‑2 (MQ=255)                                                                                                                                                                                                 tatGGCTAATACTATTTTAAATCCTTTTAAATTGAATTACGCAAATTTTACTAATGCAAACATGTTTCAGGTTACTTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATaa                                                           >  1:125669/1‑131 (MQ=255)                                                                                                                                                                                                                                     ttCAGCAAATGTTACTAATGCCAACATGTTTCAGGTTAATTTTg                                                                                                              <  1:130751/44‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                    CATTAATGAACCGTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCG  >  NC_000913/3268738‑3269117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑