Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,262,684	C→T	G326E (GGA→GAA)	tdcD ←	propionate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,262,684	0	C	T	90.0%	16.7 / NA	11	G326E (GGA→GAA)	tdcD	propionate kinase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (3/6);  minor base G (0/1);  total (3/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

CAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACACGCGCATTTTCACTGGAAACAATTCGCTCACCACAGGAGTTAGAGCGATTATTCATTTCTGTATCAATCTCTAAGCCTAATACAGCCAAATGTTCCATGACCAGACGACGAATTAAGCTTGAATTCTCTCCTATTCCGCCGGTGAA‑TATAATTCCATCCAGGCGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGG  >  NC_000913/3262457‑3262906                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                cAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGAAATCATTTTTTATTAGTTAGTACGAATAACGGCACAAATGACCCGCGCATTTTCACTGGTAACCATTCGCTCACCACAGGAGTTAGAGCGATTATTCATTTCTGTATCACTCTCTAAGCCTAATACAGCCAAATGTTCCATGACCAGACGCCGAATTAAGCTTGAATTCTCTCCTATTTCGCCGGAGCA‑TATAATtccatcc                                                                                                                                                                                                         >  1:225720/1‑251 (MQ=255)                                                                      cnnncnnacnnnncnnnnnnnnccnnnCATTTTNANNGGAAANAATNCGCTCACCACAGGAGTTAGAGCGATTATTCATTTCTGTATCAATCTCTAAGCCTAATACAGCCAAATGTTCCATGACCAGACGACGAATTAAGCGTGAATTCTCTNCTANTGCGNCGNTGAA‑TATAATTCCATNCAGGCGACGTAATGAAGCTGCGTGTCCGGCAATAGGACGGGCAANNCNNNGNACAAAGGt                                                                                                                                              <  2:224494/218‑1 (MQ=255)                                                                                                 cATTTTCACTGGAAACAATTCGCTCACCACAGGAGTTAGAGCGATTATTCATTTCTGTATCAATCTCTAAGCCTAATACAGCCAAATGTTCCATGACCAGACGACGAATTAAGCTTGAATTCTCTCCTATTCCGCCGGTGAA‑TATAATTCCATCCAGGCGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTgcgc                                                                                                                            <  1:106750/232‑1 (MQ=255)                                                                                                     ttCACTGGAAACAATTCGCTCACCACAGGAGTTAGAGCGATTATTCATTTCTGTATCAATCTCTAAGCCTAATACAGCCAAATGTTCCATGACCAGACGACGAATTAAGCTTGAATTCTCTCCTATTTCGCCGGTGAA‑TATAATTCCATCCAGGCGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGTCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAg                                                                                                      <  1:208484/250‑1 (MQ=255)                                                                                                                                      atagagATTATTCATTTCTGTATCAATCTCTAAGCCTAAGACAGCCAAATGTTCCATGACCAGACTATGAATTAAGCTTGAATTCTCTCCTATTTCGCCGGTTAA‑TATAATTCCATCCAGGCGACGTAATGAAGCTGCTTGTCTGGCAATAGGACGGTCAAGTCGGTAAACAAATGTTTTAATTTCCAGGTTCTCGCTTTCGTGACCTTTATGGCTGTCTTTTTCCATAACACGTAAATCCGAAGAAAGAc                                                                    <  1:220725/246‑1 (MQ=255)                                                                                                                                           gatgattCATTTCTGTATCAATCTCTAAGCCTAATACAGCCAAATGTTCCATGACCAGACGACGAATTGAGCTTGAATTCTCTCCTATTTCGCCGGTGAA‑TATAATTCCATCCAGGCGACGTAATGAAGCTGCGTGTCCGGCAata                                                                                                                                                                        <  1:134771/142‑1 (MQ=255)                                                                                                                                                                       gCCTAATACAGCCAAATGTTCCATGACCAGACGACGAATTAAGCTTGAATTCTCTCCTATTTCGCCGGTGCA‑TATAATTCCATCCAGGCGACGTAATGCAGCTGCGTGTCCGGCAATATGACGGACAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTaa                                                                                  >  1:167785/1‑204 (MQ=255)                                                                                                                                                                                  ccAAATGTTCCATGACCAGACGACGATTTAAGCTTGAATTCTGTCCTATTTCGCCG‑TGAAATAAAATTCCATCAAGGGGACGTAATGAGGCTGCGTGCCCGGCAATATGACGGGCAATTCGGTGAACAAAGTTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTCCCAGAACACTTAAATCCGAAAAAAGACCGGAAAGACCTAATAACCCCGACTCTTTATTCACTACGCGTTcc                        <  1:284413/251‑1 (MQ=255)                                                                                                                                                                                               gACCAGACGACGAATTAAGCTTGAATTCTCTCCTATTTCGCCGGTGAA‑TATAATTCCATCCAGGCGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTgcgcg                                                                                                                           <  1:92617/139‑1 (MQ=255)                                                                                                                                                                                                  cAGACGACGAATTAAGCTTGAATTCTCTCCTATTTCGCCGGTGAA‑TATAATTCCATCCAGGCGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTACTAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGctct        >  1:10415/1‑251 (MQ=255)                                                                                                                                                                                                        acgaATTAAGCTTGAATTCTCTCCTATTTCGCCGGTGAA‑TATAATTCCATCCAGGCGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCtggttgn  <  1:200838/251‑2 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                CAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACACGCGCATTTTCACTGGAAACAATTCGCTCACCACAGGAGTTAGAGCGATTATTCATTTCTGTATCAATCTCTAAGCCTAATACAGCCAAATGTTCCATGACCAGACGACGAATTAAGCTTGAATTCTCTCCTATTCCGCCGGTGAA‑TATAATTCCATCCAGGCGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGG  >  NC_000913/3262457‑3262906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑