Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,260,993 C→T D476N (GAT→AAT)  tdcE ← 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,260,9930CT80.0% 10.8 / 0.2 10D476N (GAT→AAT) tdcE2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (4/4);  total (5/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.87e-01

GTTTTCGTCACGGATTGGTTTCACGCGGGCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACAC  >  NC_000913/3260751‑3261236
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   
nttttCGTCACGGATTGGTTTCACGCGGGCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCCGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATTCAGCAcgt                                                                                                                                                                                                                                             >  1:206047/2‑251 (MQ=255)
                                   tGATGGCAGACAGGGAGTCCGTCGCCCCCGACAGGCCCGCGATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGGAGTGAATGATATTCAGCGCGCTGATGTATTGCACCGCCAGCCAGTCCATGAAGTGAGCGATGCTTTGCATCACTTTGTGGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTg                                                                                                                                                                                                           <  1:225724/250‑1 (MQ=39)
                                                                                             ncGATAGACATCACGATCGTGAAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATTCAGCACGTCGTCCATCAGCgg                                                                                                                                                                                                                                >  1:261226/2‑171 (MQ=255)
                                                                                                                tGCAGCGCCATCAGCGAAGCTTCGTAGCTTTACTTGTCGTGCAGGTAGTGAATGATATTCAGCGCGCTGATTTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGGCCATCACTTTGTCGTAATTCAGCACGTCGTCCATCAGCGGGGCTGTATGCGGCCCGACCAGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAcn                                                                                                                                   <  1:31831/245‑2 (MQ=255)
                                                                                                                            aGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATTCAGCACGTCGTCCATCa                                                                                                                                                                                                                                    <  1:181136/136‑1 (MQ=255)
                                                                                                                                 aGNNNNNTAGNTTTACTTGTCGGGCATGTAGTGAATGATATTCAGCGCGCGGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATTCAGCACGTCGTCCATCAGCGGTGCTGtttt                                                                                                                                                                                                                       <  2:206047/144‑1 (MQ=255)
                                                                                                                                                                   aTGATATTCAGCGCGCAGATGTAATGCACCGCCCGCCAGTCAATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATTCAGCACGCCGTCCATCAGCGGCGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCAACCCCGCCGTTAATTGCGTAGAGCCGCGTTTTCGCCAGGTTAGCGCGTGCCACAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGc                                                                                   >  1:99189/1‑242 (MQ=255)
                                                                                                                                                                                               ccGCCAGCCAAGCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATTCAGCAcgtcgcc                                                                                                                                                                                                                                         <  1:304829/64‑3 (MQ=255)
                                                                                                                                                                                                                    naTCGAGGCTGTCCATCACTTTGTCGTAATTCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTa                                                                                                                                                                    >  1:198195/2‑112 (MQ=255)
                                                                                                                                                                                                                                           tCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATATTCAGCTTCTCGTCCACCCCGCCGTTAATTGAGGAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATAACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGACAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGacac  >  1:296625/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   
GTTTTCGTCACGGATTGGTTTCACGCGGGCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACAC  >  NC_000913/3260751‑3261236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑