Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	360,774	C→T	R261R (CGC→CGT)	cynX →	cyanate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	360,774	0	C	T	91.7%	24.0 / ‑3.2	12	R261R (CGC→CGT)	cynX	cyanate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (6/5);  total (6/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

CGCGTGGTATTCACTCCCCGCGCGTGGACGCTGGGTGTTTACTTCGGTCTGATTAACGGCGGTTACGCCAGCCTGATTGCCTGGTTACCCGCTTTCTATATTGAGATTGGTGCCAGCGCGCAGTACAGCGGTTCCTTACTGGCATTGATGACGCTTGGGCAAGCCGCAGGAGCTTTGCTGATGCCTGCTATGGCTCGCCATCAGGATCGGCGCAAACTGTTAATGCTGGCGCTGGTGTTACAACTGGTGGGGTTCTGCGGCTTTATCTGGCTGCCGATGCAATTGCCGGTATTGTGGGCGATGGTGTGTGGGTTAGGTCTGGGCGGCGCGTTTCCGCTCTGTTTGCTGCTGGCGCTCGATCACTCTGTGCAACCGGCTATTGCTGGCAAGCTGGTGGCGTTTATGCAGGGAATCGGTTTTATCATCGCCGGGCTTGCCCCGTGGTTT  >  NC_000913/360562‑361008                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           cgcgTGGTATTCACTCCCCGCGCGTGGACGCTGGGTGTTTACTTCGGTCTGAGTAACGGCGGTTACGCCAGCCTGATTGCCTGGTTACCCGCTTTCTATATTGAGATTGGTGCCAGCGCGCAGTACAGCGGTTCCTTACTGGCATTGATGACGCTTGGGCAAGCCGCAGGAGCTTTGCTGATGCCTGCTATGGCTCGCCATCAGGATCGGCGCAAACTGTTAATGCTGGCGCTGGTGTTACAACtggtgg                                                                                                                                                                                                       <  1:147624/250‑1 (MQ=255)                                                                   gCCAGCCTGATTGCCTGGTTACCCGCTTTCTATATTGAGATTGGTGCCAGCGCGCAGTACAGCGGTTCCTTACTGGCATTGATGACGCTTGGGCAAGCCGCAGGAGCTTTGCTGATGCCTGCTATGGCTCGCCATCAGGATCGGCGTAAACTGTTAATGCTGGCGCTGGTGTTACACCTGGTGGGGTTc                                                                                                                                                                                                  >  1:209043/1‑189 (MQ=255)                                                                                 cTGGTTACCCGCTTTCTATATTGAGATTGGTGCCAGCGCGCAGTACAGCGGATCCTTACTGGCATTGAGGACGCTTGGGCAAGCCGCAGGAGCTTTGCTGATGCCTGCTATGGCTCGCCATCAGGATCGGCGTAAACTGTTAATGCTGGCGCTGGTGTTACAACTGGTGGGGTTCTGCGGCTTTATCTGGCTGCCGATGc                                                                                                                                                                         <  1:322018/200‑1 (MQ=255)                                                                                                                           gTACAGCGGTTCCTTACTGGCATTGATGACGCTTGGGCAAGCCGCAGGAGATTTGCTGATGCCTGCTATGGCTCGCCACCAGGATCCGCGTAAACTGTTAATGCTGGCGCTGGTGTTACCAATGGAGGGGTTCTGCGGCTTTATCTGGCTGCCGATGCAATTGCCGGTATTGTGGGCGATGGTGTGTGGGTTAGGTCTGGGCGGCGCGTTTCCGCTCTGTTTGCTGCTGgc                                                                                                >  1:135273/1‑231 (MQ=255)                                                                                                                               aGCGGTTCCTTACTGGCATTGATGACGCTTGGGCAAGCCGCAGGAGCTTTGCTGATGCCTGCTATGGCTCGCCATCAGGATCGGCGTAAACTGTTAATGCTGGCGCTGGTGTTACAACTGGTGGGGTTCTGCGGCTTTATCTGGCTGCCGATGCAATTGCCGGTAGTGTGGGCGATGGTGTGTGGGTTAGGTCTGGGCGGCGCGTTTCCGCTCTGTTTGCTGCTGGCGCTCGATCACTCTGTGCAACCggn                                                                        <  1:30386/251‑2 (MQ=255)                                                                                                                                   gTTCCTTACTGGCATTGATGACGCTTGGGCAAGCCGCAGGAGCTTTGCTGATGCCTGCTATGGCTCGCCATCAGGATCGGCGTAAACTGTTAATGCTGGCGCTGGTGTTACAACTGGTGGGGTTCTGCGGCTTTATCTGGCTGCCGATGCAATTGCCGGTATTGTGGGCGATGGTGTGTGGGTTAGGTCTGGGCGGCGCGTTTCCGc                                                                                                                <  1:114419/207‑1 (MQ=255)                                                                                                                                             ggCAGTGATGACGCTTGGGCAAGCCGCAGGAGCTTTGCTGCTGCCTGCTCTGGCTCGTCATCGGGATCGGCGTAAAGTGTTAATGCTGGCGCTGGTGTTACAACTGGTGTGGTTCTGCGGCTTTATCTGGCTGCCGATCGAATTGCCGGTTTTGTGGGCGATGGTGTGTGGGTTCGGGCTGGGCGGCGCGTTTCCGCTCTGTTTGCTGCTGGCGCTCGTTCACTCTGTGCAACCGGCTATTGCTGGCAAGc                                                          >  1:145888/1‑251 (MQ=255)                                                                                                                                                    atgaCGCTTGGGCAAGCCGCAGGAGCTTTGCTGATGCCTGTTATGGCTCGCCATCAGGATCGGCGTAAACTGTTAATGCTGGCGCTGGTGTTACAACTGGTGGGGTTCTGCGGCTTTATCTGGCTGCCGATGCAATTGCCGGTATTGTGGGc                                                                                                                                                      <  1:107708/152‑1 (MQ=255)                                                                                                                                                                                         cTGCTATGGCTCGCCATCAGGATCGGNGTAAACNNTTNATGCTGGCGCTGGTGTTACNACNNNTNNNGNTCTGCNGCNNTATCTGGCTNCCGATGCAATTGCCGGTATTGTGGGCGATGGTGTGTGGGTTAGGTCTGGGCGGNGCGTTTCCg                                                                                                                 >  2:69902/1‑152 (MQ=255)                                                                                                                                                                                         cTGCTATGGCTCGCCATCAGGATCGGCGTAAACTGTTAATGCTGGCGCTGGTGTTACAACTGGTGGGGTTCTGCGGCTTTATCTGGCTGCCGATGCAATTGCCGGTATTGTGGGCGATGGTGTGTGGGTTAGGGCTGGGCGGCGCGTTTCCg                                                                                                                 <  1:69902/152‑1 (MQ=255)                                                                                                                                                                                                  cTCGCCATCAGGATCGGCGTAAACTGTTAATGCTGGCGCTGGTGTTACAACTGGTGGGGTTCTGCGGCTTTATCTGGCTGCCGATGCAATTGCCGGTATTGTGGGCGATGGTGTGTGGGTTAGGTCTGGGCGGCGCGTTTCCGCTCTGTTTGCTGCTGgcgc                                                                                              >  1:14390/1‑162 (MQ=255)                                                                                                                                                                                                     gCCATCAGGATCGGCGTAAACTGTTAATGCTGGCGCTGGTGTTACAACTGGTGGGGTTCTGCGGCTTTATCTGGCTGCCGATGCAATTGCCGGTATTGTGGGCGATGGTGTGTGGGTTAGGTCTGGGCGGCGCGTTTCCGCTCTGTTTGCTGCTGGCGCTCGATCACTCTGTGCAACCGGCTATTGCTGGCAAGCTGGTGGCGTTTATGCAGGGAATCGGTTTTATCATCGCCGGGCTTGCCCCGTGGttt  >  1:112290/1‑251 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           CGCGTGGTATTCACTCCCCGCGCGTGGACGCTGGGTGTTTACTTCGGTCTGATTAACGGCGGTTACGCCAGCCTGATTGCCTGGTTACCCGCTTTCTATATTGAGATTGGTGCCAGCGCGCAGTACAGCGGTTCCTTACTGGCATTGATGACGCTTGGGCAAGCCGCAGGAGCTTTGCTGATGCCTGCTATGGCTCGCCATCAGGATCGGCGCAAACTGTTAATGCTGGCGCTGGTGTTACAACTGGTGGGGTTCTGCGGCTTTATCTGGCTGCCGATGCAATTGCCGGTATTGTGGGCGATGGTGTGTGGGTTAGGTCTGGGCGGCGCGTTTCCGCTCTGTTTGCTGCTGGCGCTCGATCACTCTGTGCAACCGGCTATTGCTGGCAAGCTGGTGGCGTTTATGCAGGGAATCGGTTTTATCATCGCCGGGCTTGCCCCGTGGTTT  >  NC_000913/360562‑361008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑