Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,397,444	G→A	Q908* (CAA→TAA)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,397,444	0	G	A	100.0%	39.8 / NA	15	Q908* (CAA→TAA)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/5);  total (10/5)

GATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTG  >  NC_000913/2397197‑2397635                                                                                                                                                                                                                                                        |                                                                                                                                                                                                aagacgggtgctcttccgatcggggATCCAGTCTTCTTAAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCTCTTGGAGGATGGTCAACAGGATCTCAATCAGTTCCGGTGATATCCAACTCAttattg                                                                                                                                                                                              <  1:157825/229‑1 (MQ=255)                                                                                      cAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGcn                                                                                                                      <  1:64846/238‑2 (MQ=255)                                                                                          cAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGCCAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGcc                                                                                                                                                                                          >  1:149089/1‑166 (MQ=255)                                                                                                        ncGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCaa                                                                                                                                                                          >  1:37782/2‑168 (MQ=255)                                                                                                                 ttCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATAcn                                                                                                                                  <  1:96022/199‑2 (MQ=255)                                                                                                                                         ngTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGCTGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTa                                                       >  1:66910/2‑250 (MQ=255)                                                                                                                                         cGTTCGCCAAAGCTCATGAATGCCCCNCAGGTGNNAANCACCAGCAGGATCACCACCNCTNNNANNNTNGTCAGNAGNNTCTCAATCANTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCNCCAGCCAGCNNCGGAGCAATGCCGGACATACCNATCNNCNNGNCCAnccn                                                                                                                >  2:70352/1‑191 (MQ=255)                                                                                                                                         cGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGc                                                                              <  1:70352/227‑1 (MQ=255)                                                                                                                                                                         gACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACAcc                      >  1:275849/1‑251 (MQ=255)                                                                                                                                                                                 ccaGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCg                                                                          >  1:71510/1‑191 (MQ=255)                                                                                                                                                                                   agcagGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCACTCAGTTCCGGTTCTATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGCTGCGCGCCAGCCAGCACCGGCGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAAACGGCAGCGTGACCGTGTTGCCATCGTAACTAAAAGAGACGCGTGTACCTGCGTTCACACCCAACTTcg              >  1:51848/1‑249 (MQ=255)                                                                                                                                                                                             cnaccGCTTTGAGGATGGTCAGCAGGATCTACATAAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTCCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGCTCACACCCAACTTCGCGGCATCCGCTg  >  1:254669/1‑251 (MQ=255)                                                                                                                                                                                                          gATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGNCGTNNNNNCtnnnnnnnnnn                                                                                      >  2:193942/1‑144 (MQ=255)                                                                                                                                                                                                          gATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGTCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGcn   <  1:193942/237‑2 (MQ=255)                                                                                                                                                                                                                                 gTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGTCAGCACCGGAGTAACGCCGGACATGCCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTTCCTGCGTTCACACCCAAc                  <  1:56498/199‑1 (MQ=255)                                                                                                                                                                                                                                         gATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGAAATACCCATCGGCAAGCCCCCCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGGCCGTGTTGCCATCGTAACTAAAGGAGACGAGTGTACCTGCGTTCACACCCACCTTAGCGGCa         >  1:206391/1‑200 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                GATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTG  >  NC_000913/2397197‑2397635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑