Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	326,152	G→A	P366S (CCA→TCA)	betA ←	choline dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	326,152	0	G	A	90.9%	22.9 / ‑3.4	11	P366S (CCA→TCA)	betA	choline dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (4/6);  total (4/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

ATCTCGCGGGTGATGCGAATTGCGTCGCGGAACTCCTGCCAGTCCTGCTCGTGCGACATGTAGTTAAACAGAATCGCCGGATGCTGGTGCGGGTCGCGGGATTTAATCCGCACATGCCCACGGCTTGGCGAGCGCATTGAGCCGACGTGGCACTGGAAACCGTGCTCTTTCACTGCATTCGAGCCGTTATAGTTAATCGCTACTGGCAGGAAATGGTACTGAATATTCGGCCACGCAAATTCCTCACGGCTGCGAATAAATCCACCTGCTTCAAAGTGGTTGCTGGCACCAACGCCAGTGCCGCCAAACAGCCACTCCGCACCGATTTTCGGCTGGTTCCACCACTGCAGGGCAGGGTAGAGGGAAACCGGTTCTTTGCACTCATATTGCAGATACATCTCCAGATGATCCT  >  NC_000913/325947‑326358                                                                                                                                                                                                              |                                                                                                                                                                                                               aTCTCGCGGGAGATGCGAATTGCGTCGCGGAACTACTGCCAGTCCTGCTCGTGCGACATGAAGTTAAACAGAATAGCCGGATGCTGGTGCGGGTCGCGGGATTTAATCAGCACATGCCCACGGCTTGGCGAGCGCATTGAGCCGACGTGGCACTGGAAACCGTGCTCTTTCACTGCATTCGAGCCGTTATAGTTAATCGCTACTGACAGGACATGGTACTGAATATTCGGCCACGCAAATTCCTCACGGCt                                                                                                                                                                   >  1:276423/1‑251 (MQ=255)                   aTTGCGTCGCGGAACTCCTGCCAGTCCTGCTCGTGCGACATGTAGTTAAACAGAATCGCCGGATGCTGGTGCGGGTCGCGGGATTTAATCCGCACATGCCCACGGCTTGGCGAGCGCATTGAGCCGACGTGGCACTGGAAACCGTGCTTTTTCGCTGCATTCGAGCCGTTATAGTTAATCGCTACTGGCAGGAAATGGTACTGAATATTCGGCCACGCAAATTCCTCACGGCTGCGAATAAATCCACCTGc                                                                                                                                                 <  1:153490/251‑1 (MQ=255)                                                    tGCGACATGTAGTTAAACAGAATCGCCGGATGCTGGTGCGGGTCGCGGGATTTAATCCGCACATGCCCACGGCTTGGCGAGCGCATTGAGCCGACGTGGCACTGGAAACCGTGCTCTTTCACTGCATTCGAGCCGTTATAGTTAATCGCTACTGACAGGAAATGGTACTGAATATTCGGCCACGCAAATTCCTCACGGCTGCGAATAAATCCACCTGCTTCAAAGTGGTTGCTGGCACCAACGCCAGTgcc                                                                                                                >  1:271497/1‑251 (MQ=255)                                                                                          cGGGTCGCGGGATTTAATCCGCACATGCCCACGGCTTGGCGAGCGCATTGAGCCGACGGGGCACTGGAAACCGTGCTCTTTCACTGCATTCGAGCCGTTATAGTTAATCGCTACTGACAGGAAATGGTACTGAATATTCGGCCACGCAAATTCCTCACGGCTGCGAATAAATCCACCTGCCTCAAAGTGGTTGCTGGCACCAACGCCAGTGCCGCCAAACAGCCACTCCGCACCGATTTTCGGCTGGTTcc                                                                          <  1:112773/251‑1 (MQ=255)                                                                                            ggTCGCGGGATTTAACCCGCACATGCCCACGGCTTGGCGAGCGCATTGATCCGACGTGGCACTGGAAACCGTGCTCTTTCACTGCATTCGAGCCGTTATAGTTAATCGCTACTGACAGGAAATGGTACTGAATATTCGTCCACGCAAATTCCTCACGTCTGCGAATAAATCCACCTGCTTCAAAGTGGTTGCTGGCACCAACGCCAGTGCCGCCAAACAGCCACTCCGCACCGATTTTCGGCTGGTTccac                                                                        <  1:189594/251‑1 (MQ=255)                                                                                                gcgGGATTTAATCCGCACATGCCCACGGCTTGGCGAGCGCATTGAGCCGACGTGGCACTGGAAACCGTGCTCTTTCACTGCATTCGAGCCGTTATAGTTAATCGCTACTGACAGGAAATGGTACTGAATATTCGGCCACGCAAATTCCTCACGGCTGCGAATAAATCCACCTGCTTCAAAGTGGTTGCTGGCACCAACGCCAGTGCCGCCAAACAGCCACTCCGCACCGATTTTCGGCTGGTTCCACCACt                                                                    <  1:3943/251‑1 (MQ=255)                                                                                                        tAATCCGCACATGCCCACGGCTTGGCGAGCGCATTGAGCGGACGGGGCACTGAAAACCGTCCTCTTGCACTTCAGTCGAGCCGTTATAGTTAATCTCTACTGACAGGAAATGGTACTGAATATGCGGCCACGCAAATTCCGCACGGCTGCGAATAAATCCACCTGCTTCAAAGTGGTTGCTgg                                                                                                                                <  1:46257/183‑1 (MQ=255)                                                                                                                                         ttGAGCCGACGTGGGTCTGGAAACCGTGCTCGTTCACTGCATTCGAGCCGTTATAGTTAATCGCTACTGACAGGAAATGGTACTGAATATTCGGCCACGCAAATTCCTCACGTCTGCGAATAAATCCACCTGCTTCAAAGTGGTTGCTGGCACCAACGCCAGTGCCGCCAAGCAGCCACTCCGCGCCGATGTTCGGGTGGTTCCACCACTGCGGGGCAGGGTAGAGGGAAACCGGTTCTTTGCACTCata                            <  1:133743/250‑1 (MQ=255)                                                                                                                                                       cACTGGAAACCGTGCTCTTTCACTGCATTCGAGCCGTTATAGTTAATCGCTACTGACAGGAAATGGTACTGAATATTCGGCCACGCAAATTCCTCACGGCTGCGAATAAATCCACCTg                                                                                                                                                  >  1:70940/1‑118 (MQ=255)                                                                                                                                                       cACTGAAAACCGGGCTCTTTCACTGCATTNGAGCCGTTANNGTNAATCGNTNNNGNNNGGNAATGGTACGGAATATTCGGNCANNCAAATTNCTCACGGCTGCGAATAAATCCACCTg                                                                                                                                                  <  2:70940/118‑1 (MQ=255)                                                                                                                                                                  gTGCTCTTTCACTGCATTCGAGCCGTTATAGTTAATCGCTACTGACAGGAAATGGTACTGAATATTCGGCCACGCAAATTCCTCACGGCTGCGAATAAATCCACCTGCTTCAAAGTGGTTGCTGGCACCAACGCCAGTGCCGCCAAACAGCCACTCCGCACCGATTTTCGGCTGGTTCCACCACTGCAGGGCAGGGTAGAGGGAAACCGGTTCTTTGCACTCATATTGCAGATACATCTCCAGATGATCCt  <  1:171132/251‑1 (MQ=255)                                                                                                                                                                               gCATTCGAGCCGTTATAGTTAATCGCTACTGACAGGAAATGGTACTGAATATTCGGCCACGCAAATTCCTCACGGCTGCGAATAAATCCACCTGCTTCAAAGTGGTTGCTGGCACCAACGCCAGTGCCGCCAAACAGCCACTCCGCa                                                                                             >  1:216974/1‑147 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                                                                               ATCTCGCGGGTGATGCGAATTGCGTCGCGGAACTCCTGCCAGTCCTGCTCGTGCGACATGTAGTTAAACAGAATCGCCGGATGCTGGTGCGGGTCGCGGGATTTAATCCGCACATGCCCACGGCTTGGCGAGCGCATTGAGCCGACGTGGCACTGGAAACCGTGCTCTTTCACTGCATTCGAGCCGTTATAGTTAATCGCTACTGGCAGGAAATGGTACTGAATATTCGGCCACGCAAATTCCTCACGGCTGCGAATAAATCCACCTGCTTCAAAGTGGTTGCTGGCACCAACGCCAGTGCCGCCAAACAGCCACTCCGCACCGATTTTCGGCTGGTTCCACCACTGCAGGGCAGGGTAGAGGGAAACCGGTTCTTTGCACTCATATTGCAGATACATCTCCAGATGATCCT  >  NC_000913/325947‑326358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑