Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,003,264	C→T	E115K (GAA→AAA)	fliC ←	flagellar filament structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,003,264	0	C	T	100.0%	34.1 / NA	14	E115K (GAA→AAA)	fliC	flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/10);  total (4/10)

CACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTG  >  NC_000913/2003033‑2003490                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                   cACCAAAAGCAGTTTCTGGAGCCCTAGTGGTAATTGTATCGTTATTTGTAACGCTAAAACAATCAAGGCCAAGAGTTTTAGCCTCAATCTGCTTTATAGCGATAGTGATAGTCGGGTTATCATTTGCTCCAACCTGGTTTTTAATGGAACCATTTTTTGCCAGCACGTTAACGCCGTTGAACTTGGTCTGACCAGATACGCGCTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGa                                                                                                                                                                                                                 <  1:169320/251‑1 (MQ=255)                                           tATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTccg                                                                                                                                                                        <  1:309878/250‑3 (MQ=255)                                                    ngCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGCGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGa                                                                                                                                                                                                                            >  1:62715/2‑189 (MQ=255)                                                                                      attCTGCTTCAGATCGATAGTGGTAGTCTGGTTATCAGTTGGGCCAACCGGGATTTTCATGTAGCCATTTTTTGCCGGCACGGTCACGCCGTGTAACTGGGTCTGACCAGATACGCGGGCAATTGTTTCCAGACGGGGTTTAATTTTGGCCTGGATAGAAGACAGAGCAGACTCAGAATTAGTACCGGTAGTCGCCTGCACCATCAGTTCTCTCACGCGCTGTAAGTTGTTGTTGATTTCGGACAgcgcgc                                                                                                                            <  1:153825/249‑1 (MQ=255)                                                                                              tCAGATCGATAGTGATAGTCTGGTTATCATTTGCACCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCggt                                                                                                                     <  1:275459/250‑1 (MQ=255)                                                                                                    cGATAGTGTTAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCATACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGAGTTCGGACAGCGCGCCTTCGGTGGTCtn                                                                                                               <  1:40635/250‑2 (MQ=255)                                                                                                         gtgatagtCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGCCCTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAgc                                                                                                                                >  1:190644/1‑228 (MQ=255)                                                                                                                                                               gCCAGCACGTTCACGCCGTTGAACTGNGTCTGANNAGNTACGCGGTCAATTTCATCCNGANNNGNNNTNATTTTNTCNNGGCTAGAAGNCAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCCCGCCNACGATGCAANNTGTTGTTGATTTCGGACAGCGNGCCNNCNNGNGTCNgcn                                                                                                             >  2:75076/1‑192 (MQ=255)                                                                                                                                                                      cgtgcACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACgg                                                                                                       <  1:55000/188‑1 (MQ=255)                                                                                                                                                                          cACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGATAGATCAGACTCAGAGTTAGTACCGGTAGTTGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCTTCGTTGGCGTTACGGGCCGCCTGAGTCAg                                                                  <  1:221902/225‑1 (MQ=255)                                                                                                                                                                             gCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACTCGCTGTAAGTTGTTGTTGATTTCGGACAGCTCGTCTTCGGTGGTCTTCGCAACGGAGATACTGTCGTTGGCGTTACGGGCCGCCTGAGTGAGGCCTTTAATGTTAGAGGTGAAACGGTTAg                                     <  1:129652/251‑1 (MQ=255)                                                                                                                                                                                          gTCTGANCAGATACGCGNTCANNTTCANCNANACGGGATTTAATTTTGTCCTGGATAGAAGNCAGATCAGACTCAGAGTTAGTACNNGTNNNGGNNTGTAc                                                                                                                                                                              <  2:234881/101‑1 (MQ=255)                                                                                                                                                                                          gTCTGACCAGATAAGAGGTCAATTTCATCCAGACGGGATTTAATTTTGTCGTGGATAGAAGACAGATCAGACTCCGAGTTAGTACCGGTAGTGGCCTGTAc                                                                                                                                                                              >  1:234881/1‑101 (MQ=255)                                                                                                                                                                                                                 ttCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTg  <  1:75076/250‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                   CACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTG  >  NC_000913/2003033‑2003490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑