Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,827,165 G→A C365C (TGC→TGT)  astB ← N‑succinylarginine dihydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,827,1650GA80.0% 12.7 / ‑2.5 10C365C (TGC→TGT) astBN‑succinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (4/4);  total (5/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

CCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGCGTTCTTCTTCTGTCAATACCACCCGCAACCGCAGGCACGCCGGGCCGCCGCCATTCGCCATGCTTTCACGTAAATCAAAGACTTTTAGTTCGCTAATCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTAT  >  NC_000913/1826927‑1827401
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            
ccccTCCCGCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGTAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGGATCGTTCATCATCACCGCCGGATTCACCGCCCGGCGTTCTTCTTCTGTCAATACCACCCGCAACCGCAGACACg                                                                                                                                                                                                                                          <  1:242168/243‑1 (MQ=255)
 ncctccctcGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGCGTTCTTCTTCTGTCAATACCACCCGCAACCGCAGGCACGCCGGgccgc                                                                                                                                                                                                                                 >  1:203721/2‑251 (MQ=255)
                                                                                              gTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGCGTTCTTCTTCTGTCAATACCACCCGCAACCGCAGACACGCCGGGCCGCCGCCATTCGCCATGCTTTCACGTAAATCAAAGACTTTTAGTTCGCTAATCGGGTTGTCAGCGACAAGGAGTTCATTGAGATAACCCCATACTc                                                                                                                                    >  1:12387/1‑251 (MQ=255)
                                                                                                                  nnnnnnnnnnccnNNNNTAANTATCCACCCAGGCTTTGAGCGCATTAACCAGCGTTTCGTTCATCATCACCGCCGGATGCCCCGCCCGGGGTTCTTCTTCTGTCAATCCCCCCCGCAACCGCAGGCACGCCGGGCCGCCGCCATGCGCCATGCt                                                                                                                                                                                                                 <  2:203721/143‑1 (MQ=255)
                                                                                                                                   taACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGCGTTCTTCTTCTGTCAATACCACCCGCAACCGCAGACACGCCGGGCCGCCGCCATTCGCCATGCTTTCACGTAAATCAAAGACTTTTAGTTCGc                                                                                                                                                                                    <  1:142276/166‑1 (MQ=255)
                                                                                                                                                    aTTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGCGTTCTTCTTCTGTAAATACCAACCGCAACCGCAGACACGAAGGGCCGCCGCCATTCGCCATGCTTTCCCGTAAATCAAAGACTTTTAGTTCGCTAATCGGGTTGTCAGCGGCAAGGAGTTCATTg                                                                                                                                                    >  1:170088/1‑181 (MQ=255)
                                                                                                                                                            catgaaaCAGCGTTTCGTTCATCATCACCGCCGGATTCTCCGCCCGGCGTTCTTCTTCTGTCAATACCCCCCGCAACCGCAGACACGCCGGGCCGCCGCCATTCGCCATGCGTTCACGTAAATCAAAGACGTTTAGTTCGCTAATCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATATCCCCATACTCCGGCGTGTTCCCGACACTCCGGAGGCAGCACCAACACCATGGAACCATCATCGCGGCTcagn                                                                      <  1:80681/247‑2 (MQ=255)
                                                                                                                                                              ttAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGCGTTCTTCTTCTTTCAATACCACCCGCAACCGCAGACACGCCGGGCCGCCGCCATTCGCCATGCt                                                                                                                                                                                                                 <  1:316811/110‑1 (MQ=255)
                                                                                                                                                                                                                           aTACCACCCGCAACCGCAGACACGCCGGGCCGCCGCCATTCGCCATGCTTTCACGTAAATCAACGACTTTTAGTTCGCTAATCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGAGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGAAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACt       >  1:169886/1‑251 (MQ=255)
                                                                                                                                                                                                                                accCGCAACCGCAGACACGCCGGGCCGCCGCCATTCGCCATGCTTTCACGTAAATCAAAGACTTTTAGTTCGCTAATCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTAt  >  1:146746/1‑251 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            
CCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGGCGTTCTTCTTCTGTCAATACCACCCGCAACCGCAGGCACGCCGGGCCGCCGCCATTCGCCATGCTTTCACGTAAATCAAAGACTTTTAGTTCGCTAATCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTAT  >  NC_000913/1826927‑1827401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑