Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	323,514	G→A	G393R (GGG→AGG)	ykgF →	putative amino acid dehydrogenase with NAD(P)‑binding domain and ferridoxin‑like domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	323,514	0	G	A	100.0%	17.3 / NA	8	G393R (GGG→AGG)	ykgF	putative amino acid dehydrogenase with NAD(P)‑binding domain and ferridoxin‑like domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/2);  total (6/2)

CTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAG  >  NC_000913/323299‑323709                                                                                                                                                                                                                        |                                                                                                                                                                                                    cTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTTACCCGGGACAATTGGGGCGGTGGTTTCTCCGCTACTTGGGGGCTATAAAGATTTTAAAGATTTAACCTAAGCCTGCTCTTTATGTCCAGCTTGTTACCACGTGTGTCCGGGGGGTATTCAGCTGTGAAAACTGATTTTGCGTGCTCATCGGGGGATGGCTGAAAAAAGGATCACCGCAAAAACACAGCAACAGGCGAaaaa                                                                                                                                                                   >  1:295954/1‑250 (MQ=255)                                                                     gTGATTTCTCCGCTCCTTGGCGGCTATAAAGATTTTAACGATTTACCCTACGCCTGCTCTTTATGCAAAGCTTGTGACAACGTGTGTCCGGTGCGTATTACGATGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAAGGATCACCGCAAAAGAAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGAGGAAAGTCGGGATGATGGCCGGTGCTAATGCGGc                                                                                              >  1:236264/1‑251 (MQ=255)                                                                            ctcCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAAGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTgg                                                                                       >  1:283741/1‑251 (MQ=255)                                                                                                                        gCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGTGTATTCCGCTGTCAAAACTGATTGTGCGTCATCGTCGGGGGATGTCTGAAAAAAGGATCACCGGAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATgn                                                                                                                                                    <  1:60193/146‑2 (MQ=255)                                                                                                                         ccTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAAGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGc                                          <  1:99146/251‑1 (MQ=255)                                                                                                                                                                   gTATTCCGCTGTCCAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAACAGGATCACCGCAAAAGCAGAGCAACGGGAGATAAAAATGTTCGCTTCTGCCAATAGTCATCCAGGATTGTGGAAAGTTGGGATGATGGCCGGTGCTCATGCGGCACGCTGGTTTATCAATGACGGCAAAACACCACTCAAATTTGGCGCGATTAGAGCCTGGATGGAAGCACACGATCTTCCTGACGCTGACGgag  >  1:44286/1‑249 (MQ=255)                                                                                                                                                                   gTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAAGGATAACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGATTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACACGATCTTCCTGAAGCTGACGgag  >  1:44299/1‑249 (MQ=255)                                                                                                                                                                             gTCAAAACTGATTTTGCATCATCGTCGGGTGATGGCTGAAAAAAGGATCACCGAAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCt         >  1:107050/1‑232 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                    CTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAG  >  NC_000913/323299‑323709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑