Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,824,672	G→A	A89V (GCG→GTG)	ves ←	HutD family protein Ves

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,824,672	0	G	A	100.0%	18.0 / NA	8	A89V (GCG→GTG)	ves	HutD family protein Ves
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/3);  total (5/3)

CGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAAC  >  NC_000913/1824460‑1824860                                                                                                                                                                                                                     |                                                                                                                                                                                             cGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTACCACGCCCCGTTACTCACAAACACCACCCCGGCGCGTGCGCCAAACGTAGTACACGTACGTTCGGCACTTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCACCGCAAAGGCAAAAGCCTGGAACGGTTTTAAGGTATgg                                                                                                                                                        >  1:228938/1‑251 (MQ=255)            gcgcCCTGGTCGTGAGTGAGCAATTTACCGCCCAGTTGCCACGCCCCTTTAATCACAAACAGCCCCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTGACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCTACATCTGCCCTGCCGTCAGTTGCGCTTTTACCTCCTGGTCGACTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTc                                                                                                                                             <  1:284583/251‑1 (MQ=255)                             gAGCAATTTATCGCCCAGTTGCCACGNCCCGTTNNTCNCAAACACCACCCCGCCGCGNGANNNANNNGNCGTAANCGNNCGTTCGGCANTTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCNACATCTGCCNNGCCGTCAGTTTCGCTTTTACCNCCCNNTNNANTGCnaa                                                                                                                                                                                       >  2:70592/1‑192 (MQ=255)                             gAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCACTGCAAAGGCAAAAGGCTgg                                                                                                                                                                          <  1:70592/205‑1 (MQ=255)                                                                                         gCCAAAGGGAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCACTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTccc                                                                >  1:57074/1‑251 (MQ=255)                                                                                           cAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCACTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCa                                                                                                                            >  1:150195/1‑189 (MQ=255)                                                                                                                          gACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGCCATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCACTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTcgc                                                                                                                      >  1:108663/1‑164 (MQ=255)                                                                                                                                                        cATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCACTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAAc  <  1:6986/250‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                             CGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAAC  >  NC_000913/1824460‑1824860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑