Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,822,846	G→A	S130N (AGC→AAC)	nadE →	NH3‑dependent NAD(+) synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,822,846	0	G	A	89.5%	42.6 / ‑2.8	19	S130N (AGC→AAC)	nadE	NH3‑dependent NAD(+) synthetase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (7/10);  total (9/10)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGCAACGAATCACTGCAATTTATTGCCGTACGCCTGCCCTATGGTGTTCAGGCCGACGAACAAGATTGCCAGGATGCCATTGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGCGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCAGGCATTGAACTGAGCGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCACCGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGC  >  NC_000913/1822617‑1823060                                                                                                                                                                                                                                      |                                                                                                                                                                                                                       aCGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGCAACGAATCACTGCAATTTCTTGCCGTACGCCTGCCCTATGGTGTTCAGGCCGACGAACCAGATTGCCAGGATGCCATTGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGCGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCAGGCATTGAACTGAACGATTTTGTCCGTGGCAATg                                                                                                                                                                                                    >  1:44972/1‑250 (MQ=255)        ccGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGCAACGAATCACTGCAATTTATTGCCGTACGCCTGCCCTATGGTGTTCAGGCCGGCGAACAAGATTGCCAGGATGCCATTGCCTTTATTCAACCGGATCGCGTATGAACCGTTAATATCAAGGGCGCGGTATTGGCCAGCGAACAGGCATTGCGTGAAGCAGGCATTGAACTGAACGATTTTGTCCGTGGCAATGAAAAAgcg                                                                                                                                                                                            <  1:324883/251‑1 (MQ=255)                            gCGATTCATGAGCTGCGCCTGGAAACCGGCAACGAATCACTGCAATTTATTGCCGTACGCCTGCCCTATGGTGTTCAGGCCGACGAACAAGATTGCCAGGATGCCATTGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGCGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCAGGCATTGAACTGAACGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGcac                                                                                                                                                                         >  1:88197/1‑250 (MQ=255)                                                          aaCGAATCACTGCAATTTATTGCCGTACGCCTGCCCTATGGTGTTCAGGCCGACGAACAAGATTGCCAGGATGCCATTGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGCGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCAGGCATTGAACTGAACGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCgg                                                                                                                                             <  1:27028/248‑1 (MQ=255)                                                                               nccGTACGCCTGCCCTATGGTGTTCAGGCCGACGAACAAGATTGCCAGGATGCCATTGCCTTTCTTCAACCGGATCGCGTATTAACCGTTAATCTCAAGGGCGCGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCCGGCATTGCACTGAACGATTTTGTCCGTgg                                                                                                                                                                                                         >  1:31212/2‑167 (MQ=255)                                                                                            ccTATGGTGTTCAGGCCGACGAACAAGATTGCCAGGATGCCATTGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGCGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCCGGCATTGAACTGAGCGATTTTGTTCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCACCGATCATGCAGCAGAAGCCAtt                                                                                                        >  1:315485/1‑251 (MQ=255)                                                                                                      tCAGGCCGACGAACAAGATTGCCAGGATGCCATTGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGCGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCAGGCATTGAACTGAACGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCgtggtn                                                                                                                                   <  1:83059/214‑2 (MQ=255)                                                                                                         ngCCGACGAACAAGATTGCCAGGATGCCATTGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGCGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCAGGCATAGAACTGAACGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGAc                                                                                                                                                   >  1:197064/2‑195 (MQ=255)                                                                                                                   cAAGATTGCCAGGATGCCATTGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGCGGTATTGGCCAGCGAACCGGCATTGCGGGAAGCAGGCATTGAACTGAACGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAACGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCACCGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATgg                                                                                 >  1:307489/1‑251 (MQ=255)                                                                                                                                          cTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGCGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCAGGCATTGAACTGAACGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCACCGATCATgcagc                                                                                                                  <  1:140533/195‑1 (MQ=255)                                                                                                                                             tATTCAACCGGATCTCGTATTAACCGTTAATAGCAAGGGCGCGGGATTGGCCAGCGAACAGGCATTGCGGGAAGCAGGCAGTGAACTGAACGCTTTTGTCCGTTGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCggg                                                                                                                                                         <  1:168557/153‑1 (MQ=255)                                                                                                                                                  nnnnnnnnnncgNNNNNACCNTTAATATCAAGGGCGCGGTAGTGGCCAGCGAACAGGCATTGCGGTAAGCAGGCATAGAACTGAACGATTGTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGAc                                                                                                                                                   <  2:197064/144‑1 (MQ=255)                                                                                                                                                          cgcgTATTAACCGTTAATATCAAGGGCGCGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCAGGCATTGAACTGAACGATTGTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAAGATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCACCGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGCCGGTACGGACATTAATCCGCTGTATCGTCTca                                           <  1:109741/250‑1 (MQ=255)                                                                                                                                                                              cAAGGTCGCGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCATGCATTGAACTGAACTATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGAGGTGTCg                                                                                                                                        <  1:244625/137‑1 (MQ=255)                                                                                                                                                                              cAAGGNNGCNNNATNNGACAGCGAACAGGCATTGCGGGANGCAGGCATTGAACTGAACGATTTTGTCCGNGNCNATGANNAAGNGCGTGAGCGGNTGAAAGCACAATATAGCATTGCGGGTATGACCCGCGGTGTCg                                                                                                                                        >  2:244625/1‑137 (MQ=255)                                                                                                                                                                                       ggTATTGGCCAGCGAACAGGCATTGCGGGAAGCAGGCATTGAACTGAACGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCACCGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGAcgcc                                                                          <  1:310661/190‑3 (MQ=255)                                                                                                                                                                                               ccAGCGAACAGGCATTGCGGGCAGCAGGCATTGAACTGAACGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCACCGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATcc                                                         >  1:249276/1‑199 (MQ=255)                                                                                                                                                                                                  gCGAACAGGCATTGCGGGAAGCCGGAGTTGAACAGGGCGATTTTGTCCGTAGCACTGCACAAGCGCGTGAGCGGATGACAGCACAATATAGAATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCCCCTATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGc  >  1:282548/1‑251 (MQ=255)                                                                                                                                                                                                                      gCAGGCATTGAACTGAACGATTTTGTCCGGTGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCACCGATc                                                                                                                         <  1:155992/112‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                       ACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGCAACGAATCACTGCAATTTATTGCCGTACGCCTGCCCTATGGTGTTCAGGCCGACGAACAAGATTGCCAGGATGCCATTGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGCGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCAGGCATTGAACTGAGCGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCACCGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGC  >  NC_000913/1822617‑1823060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑