Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,617,534	C→T	T169T (ACC→ACT)	ydeA →	L‑arabinose exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,617,534	0	C	T	85.7%	24.3 / ‑1.8	14	T169T (ACC→ACT)	ydeA	L‑arabinose exporter
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (6/6);  total (7/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTACACGGCATACAGCTATATCGAGCCTTTTGTACAAAAC  >  NC_000913/1617368‑1617741                                                                                                                                                                       |                                                                                                                                                                                                                cacaTGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACTttcttc                                                                                                                                                                                                           <  1:215337/173‑1 (MQ=255)     tGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCAGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTCTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACTttcttc                                                                                                                                                                                                           <  1:127954/169‑1 (MQ=255)                ngTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCCGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGAACTGCCGATGGTCTTCGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACTTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGc                                                                                                                                              >  1:34973/2‑219 (MQ=255)                                                        cgnNAAGCGAGCANAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTANCTCTCGGGCNNATNGTGGTNCNNNANNNCGNTTGGCGAATGACTTTCTTCNCGNNTGGTATNGGGGCGCTTATCACCCTTTTGTGCCt                                                                                                                                                                     <  2:74966/156‑1 (MQ=255)                                                        cggGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACTTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCt                                                                                                                                                                     >  1:74966/1‑156 (MQ=255)                                                                                                      cTGGCGATGGTCTTAGGTTTACCTGTCGGGCTCATTGTGGGGCAGTAGGTCGGCTGCCGAATGACTTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTtgt                                                                                                                                                                         <  1:163048/106‑1 (MQ=255)                                                                                                               gTCTTAGGGTTACCTCTCGGGCGCATTGTGGGCCAGTCTTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTCTTGGGGCGCTTCTCCCCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGACAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCACCCATTACACGGCATACCGCTATATCGAGcc               >  1:179165/1‑251 (MQ=255)                                                                                                                      gTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTGCGCGAGTTGTATTGGGGCGCTTATCACCCTTTTGGGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCan                                                                                                                         <  1:35548/138‑2 (MQ=255)                                                                                                                             nctcGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACTTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTACACGGCATACAGCTATATCGAGCCTTTTGTACAAAAc  >  1:194375/2‑250 (MQ=255)                                                                                                                                               ccAGTATTTCGGTTGGCGAATGACTTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCAGCATTGATGAGCATTTATTTGTTAACTGTGTTGGTTGTCAc                                                <  1:69158/186‑1 (MQ=255)                                                                                                                                               ccAGTATTTCGGTTGGCGAATGACTTNCTTCGCNNTTNGTATTGGGGCGCTTCTAACNCTNNNGNNNCNGATTANGTNNCTTCCCTTANTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTCTTGTTCCGCCGCNCGGCATTGANNAGCATTTATTTGTTAACTGTGNGGGNNGNNan                                                >  2:69158/1‑185 (MQ=255)                                                                                                                                                            tGGCGAATGACTTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGGGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGccn                                           <  1:60967/178‑2 (MQ=255)                                                                                                                                                              gCGAATGACTTTCTTAGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATtgat                                                                                 >  1:86690/1‑138 (MQ=255)                                                                                                                                                               cGAATGACTTTCTTCGCGATTGGTATTGGGGGGATTATCACCCTTTTGTGCATGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGAATACCGCTCTTGTTCGGCCGCCCGGCATTGATGAg                                                                              >  1:219670/1‑140 (MQ=255)                                                                                                                                                                       |                                                                                                                                                                                                                CACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTACACGGCATACAGCTATATCGAGCCTTTTGTACAAAAC  >  NC_000913/1617368‑1617741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑