Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,548,667	C→T	R423C (CGT→TGT)	fdnG →	formate dehydrogenase N subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,548,667	0	C	T	100.0%	23.7 / NA	9	R423C (CGT→TGT)	fdnG	formate dehydrogenase N subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/5);  total (4/5)

AAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTGCTGGCCTCCACCAGCGCACCGGATCGCACAACCACCTTCCTGTACGCGCTGGGCTGGACGCAGCACACTGTGGGTGCGCAGAACATCCGTACTATGGCGATGATCCAGTTGCTGCTCGGTAACATGGGTATGGCCGGTGGCGGCGTGAACGCATTGCGTGGTCACTCCAACATTCAGGGCTTGACTGACTTAGGCCTGCTCTCTACCAGCCTGCCAGGTTATCTGACGCTGCCGTCAGAAAAACAGGTTGATTTGC  >  NC_000913/1548431‑1548835                                                                                                                                                                                                                                             |                                                                                                                                                                         aaaaCGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTGCTGGCCTCCACCAGCGCACCGGATCGCACAACCACCTTCCTGTACGCGCTGGGCTGGACGCAGCACACTGTGGGTGCGCAGAACATCTGTACTATGGCgang                                                                                                                                                            <  1:254531/251‑1 (MQ=255)                                                                                   cacGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTTCTGGCCTCCACCAGCGCACCGGATCGCACAACCACCTTCCTGTTCGCGCTGGGCTGGACGCAGCACACTGTGGGTGCGCAGAACATCTGTACTATGGCGATGATCCAg                                                                                                                                                      <  1:218965/175‑1 (MQ=255)                                                                                               cgtTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTGCTGGCCTCCACCAGCGCACCGGATCGCACAACCACCTTCCTGTACGCGCTGGGCTGGACGCAGCACACTGTGGGTGCGCAGAACATCTGTACTATGGCGATGATCCAGTTGCTGCTCGGTAACATGGGTATGGCCGGTGGCGGCGTGAACGCATTGCGTGGTCACTCCAACATTCAGGGCTTGACTGACTTAGGcc                                                              <  1:182704/251‑1 (MQ=255)                                                                                                              cGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTGCTGGCCTCCACCAGCGCACCGGATCGCACAACCACCTTCCTGTACGCGCTGGGCTGGACGCAGCACACTGTGGGTGCGCAGTACATCTGTACTATGGCGATGATCCAGTTGCTGCTCGGTAACATGGGTATGGCCGGTGGCGGCGTGAACGCATTGCGTGGTCACTCCAACATTCAGGGCTtgact                                                                        <  1:6301/226‑1 (MQ=255)                                                                                                                   cGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTGCTGGCCTCCACCAGCGCACCGGATCACACCACCCCCTTCCTGTATGCGATGGGCTGGCCGCAGCACACTGTGGGTGCGCAGAACATCTGTACTATGGCGATGATCCAGTTGCTGCTCGGTAACATGGGTATGGCCGGTGGCGGCGTGAACGCATTGCGTGGTCAc                                                                                             >  1:210709/1‑200 (MQ=255)                                                                                                                         aaGCCGACTTCCTGAAAGTGTGTGAAGTGCTGGCCTCCACCAGCGCACCGGATCGCACAACCACCTTCCTGTACGCGCTGGGCTGGACGCAGCACACTGTGGGTGCGCAGAACATCTGTACTATGGCGATGATCCAGTTGCTGCTCGGTAACATGGGTATGGCCGGTGGCGGCGTGAACGCATTGCGTGGTCACTCCAACATTCAGGGCTTGACTGACTTAGGCCTGCTCTCTAcc                                                   >  1:308332/1‑236 (MQ=255)                                                                                                                                                             ccaccaGCGCACCGGATCGCACAACCACCTTCCTGTACGCGCTGGGCTGGACGCAGCACACTGTGGGTGCGCAGAACATCTGTACTATGGCGATGATCCAGTTGCTGCTCGGTAACATGGGTATGGCCGGTGGCGGCGTGAACGCATTGCGTGGTCACTCCAACATTCAGGGCTTGACTGACTTAGGCCTGCTCTCTACCAGCCTGCCAGGTTATCTGCCGCTGCCGTCAGAACAACAGGTTGATTTGc  >  1:306093/1‑249 (MQ=255)                                                                                                                                                                               ncacaACCACCTTCCTGTACGCGCTGGGCTGGACGCAGCACACTGTGGGTGCGCAGAACATCTGTACTATGGCGATGATCCAGTTGCTGCTCGGTAACATGGGTATGGCCGGTGGCGGCGTGAACGCATTGCGTGGTCACTCCAACATTCAGGGCTTGACTGACTTAGGCCTGCTCTCTACCAGCCTGCCAGGTTATCTGACGCTGCCGTCAGAAAAAc              >  1:93866/2‑219 (MQ=255)                                                                                                                                                                                  caACCACCTTCCGGGACGCGCTGGGCTGGACGCATCACACTTTGTGTGCGCAGAACATCTGTACTATGCCGATGATCCAGTTGCTTCTCTGTAACATGGGTAGGGCCGGTGGCGGCGTGAACGCATTGCGTGGTCACTCCAAc                                                                                       <  1:147763/143‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                         AAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTGCTGGCCTCCACCAGCGCACCGGATCGCACAACCACCTTCCTGTACGCGCTGGGCTGGACGCAGCACACTGTGGGTGCGCAGAACATCCGTACTATGGCGATGATCCAGTTGCTGCTCGGTAACATGGGTATGGCCGGTGGCGGCGTGAACGCATTGCGTGGTCACTCCAACATTCAGGGCTTGACTGACTTAGGCCTGCTCTCTACCAGCCTGCCAGGTTATCTGACGCTGCCGTCAGAAAAACAGGTTGATTTGC  >  NC_000913/1548431‑1548835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑