Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,849	C→T	E208K (GAA→AAA)	sapB ←	putrescine ABC exporter membrane subunit SapB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,849	0	C	T	90.9%	19.6 / ‑3.6	11	E208K (GAA→AAA)	sapB	putrescine ABC exporter membrane subunit SapB
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (6/4);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

AAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGA  >  NC_000913/1354668‑1355087                                                                                                                                                                                      |                                                                                                                                                                                                                                               aaaCCCGGCCAGCTCAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCa                                                                                                                                                                            >  1:110385/1‑250 (MQ=255)                               agcatcGCCAGCGTCAGCAGGGGGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCTCGACATACCGCTGGGCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTTGATAGTGCTGATACGCATCAGGCGGATCGCGACAGTTGCTGGCGCAACCGGCAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCa                                                                                                                                                    <  1:175336/242‑1 (MQ=255)                                 catcGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGTAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTTGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCAttt                                                                                                                                           >  1:234775/1‑251 (MQ=255)                                  atcGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGAGAGGAATAACCGGCGGTAACGCGATATGAGGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCCTACAATTTGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGgcg                                                                                                                                                                                                    >  1:188669/1‑193 (MQ=255)                                                                                               cgTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTTGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATgn                                                                                                                              <  1:35601/202‑2 (MQ=255)                                                                                                           cGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCAGACACTTTGATAGTGCTGATACGCATCAGGCGGATCAc                                                                                                                                                                                                                  <  1:314436/106‑1 (MQ=255)                                                                                                                                    cgACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTTGATCGTGATGATACGCATCCGGAGGATCACTTCAGTTGTTGGCGCAACCGACGGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCCTGATCATTTCATCCCGCCATGGTGCGTCCGAAAGCCAGGCATCAATCAACGCCAAACCGGTAATCGGTTTCCCTTCGTCGAGCAGATCGAAACGCCCTGAAACGGGc                                         >  1:234783/1‑250 (MQ=255)                                                                                                                                              cgGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTTGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGATTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAAGCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAg                              <  1:162178/251‑1 (MQ=255)                                                                                                                                                       gccgcTTTTACATAGTTCTGGTCATACACTTTGTTAGTGCTGATACGCATCAGGCGGATTACTTCAGTTCTTGGCGAAAACGACAGGGCGATCACGGGCAATATCATATCGCGGATTGCGCTCCTGATCCTTTCATCCCGCCATGGTGTGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCgtgagtg                      >  1:326824/1‑250 (MQ=255)                                                                                                                                                                          ggTCATACACTTTGATAGTGCTGATACGCATCAGGCGGATCGCTTCAGTTGTTGGAGAAACCGAAAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCAGCCATGGTGCGTCAGAAAGcc                                                                                                                >  1:183581/1‑141 (MQ=255)                                                                                                                                                                           gTCATACACTTTGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCACTCAACGCAAAACCGGTAATCGGTTTCACTTAGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGa  >  1:108185/1‑250 (MQ=255)                                                                                                                                                                                      |                                                                                                                                                                                                                                               AAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGA  >  NC_000913/1354668‑1355087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑