Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	100.0%	38.4 / NA	14	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/8);  total (6/8)

CGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTC  >  NC_000913/1337182‑1337635                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  cGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGt                                                                                                                                                                                                             >  1:71874/1‑251 (MQ=255)                       ccTCAAGCTGTAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCGCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCTCCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCGATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTg                                                                                                                                                                                                      <  1:230390/236‑1 (MQ=255)                                                gCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAACGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggctggn                                                                                                                                                              <  1:60700/251‑2 (MQ=255)                                                     gCTGGAACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgc                                                                                                                                                         >  1:250256/1‑251 (MQ=255)                                                                  tCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTAGCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCGGGCAACCGTAACTTGGGAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggtgg                                                                                                                                             <  1:302258/250‑1 (MQ=255)                                                                    gAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGcc                                                                                                                                                             <  1:11960/232‑1 (MQ=255)                                                                                  aTTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCTTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTGGCCTATGCGCTGGCg                                                                                                                            <  1:251910/251‑1 (MQ=255)                                                                                                                       cgaACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAatat                                                                                                                    >  1:3475/1‑222 (MQ=255)                                                                                                                                  tnAACNTNNTNNGATNCGGTTGTACCACCTGTATTGGNNACTCTGGGCNGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGNGGTGCTGTCNNGCNACCGTNANNNTNNNGGNCGTATCCATCCGCTGGTTANAANNAACTGGNTGGCCTCGCCGCCGCTGTTGGTTGcc                                                                                                                                        <  2:71874/191‑1 (MQ=255)                                                                                                                                             ggATACGGTTGTACCACCTGTATTGGAAACTCTGGGCCGCTGCCCGATACTATCGAAACGGCCATCAACAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGCAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgccgcca                                                                                                                                                    >  1:222030/1‑167 (MQ=255)                                                                                                                                             ggATACGGTGTTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGTCGATTNAACNGTCGNTGNGGTGCGGTCCGACAANCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGNNTNNNCNcgccgaca                                                                                                                                                    <  2:222030/168‑4 (MQ=255)                                                                                                                                                                            ctGGGCCTCTGCCCGATCCGATCGAAACGGCAATCAAAAAAGGCGAGGTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTTAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCAGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCTGGAAATAGGACTAGCAACCAGGCTTCTTAGCCTATCGGCCATGATCCCATAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCgg                                    <  1:312374/249‑1 (MQ=255)                                                                                                                                                                                                   naaaCGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGt              >  1:78667/2‑248 (MQ=255)                                                                                                                                                                                                             tNAAAAAAGGCGATGTAACAGTAGGTGCGGTGCTGTACGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGGTAAAAATAACTGGCTGGCATCGCTGCCGCTGGTGGTTGCCTATGCGCAGGCGGGAAATATGAATATCCACCTGGCTTCTGAGCCTATCGGCCCTGATCGCAAAGGCGAACCGGTTTATCAGAAAGATATCTGGCAATCGGCACAAGAAATTGCCAGGGCGGTCGAACaaagttc  >  1:253291/1‑245 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  CGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTC  >  NC_000913/1337182‑1337635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑