Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,290,881	C→T	L214F (CTT→TTT)	rssB →	regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,290,881	0	C	T	100.0%	24.2 / NA	10	L214F (CTT→TTT)	rssB	regulator of RpoS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/4);  total (6/4)

CGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCG  >  NC_000913/1290767‑1291027                                                                                                                   |                                                                                                                                                   cGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCAATTTCGGAAAACGATCTGGCCTTTTCTTGCCTTGATGTCCCCCGAGCTGGACATAATGGCGTATTTGCTGCATTGTTCTTACGCGCATTGTTTAACGGATTATTACAGGTACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTCAACCATTTACTTCGTCAGGCCAATCTGCCggg            >  1:234239/1‑251 (MQ=255)           ttGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGAACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCg  >  1:133144/1‑251 (MQ=255)              ccGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTtgctgc                                                                                                                                             <  1:114843/109‑1 (MQ=255)                          ncGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGAGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGa                                                    >  1:261818/2‑186 (MQ=255)                                                         ggAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTgng                                                               <  1:253708/144‑1 (MQ=255)                                                                gATCTGGCATTTTATTGCCTTGATGTCACCCNAGCNGGACNTANTGGCGTATTTGCTGCNTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTNNCNNNCNAAAATCaa                                                                                <  2:229583/120‑1 (MQ=255)                                                                gATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTCCGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCaa                                                                                >  1:229583/1‑120 (MQ=255)                                                                     ngCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAg                                                  >  1:79394/2‑145 (MQ=255)                                                                                                               cGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTgn                                                    <  1:201211/101‑2 (MQ=255)                                                                                                               cGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGa                                                    >  2:201211/1‑101 (MQ=255)                                                                                                                   |                                                                                                                                                   CGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCG  >  NC_000913/1290767‑1291027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑