Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	664,506	G→A	P229S (CCG→TCG)	rlpA ←	rare lipoprotein RlpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	664,506	0	G	A	100.0%	15.2 / NA	8	P229S (CCG→TCG)	rlpA	rare lipoprotein RlpA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/4);  total (4/4)

GCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGT  >  NC_000913/664278‑664730                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                 gcgcACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAAAGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCCGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGAATCTTCGCTTTTTAGCGt                                                                                                                                                                                                                >  1:247280/1‑247 (MQ=255)                                              ggCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTTCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGTGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTTGTGGTTACCGGCGCGCCGGTCGAATCTTCGCTTTTTAg                                                                                                                                                                                                                   <  1:242973/198‑1 (MQ=255)                                                            tGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCTGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGAATCTTCGCTTTTTAg                                                                                                                                                                                                                   <  1:186770/185‑1 (MQ=255)                                                                                                    cAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGTTCTTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGAATCTTCGCGTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTg                                                                                                                                                                                  <  1:293126/178‑1 (MQ=255)                                                                                                                                        gcgcAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGAATCTTCGCTTTTTAGCGTCGAATTACTGACCgg                                                                                                                                                                                                 >  1:6242/1‑127 (MQ=255)                                                                                                                                                        gCTGCCTTCCAGTACACCAGGCGCTAAGGTTGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGAATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCGCCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGcc                                                                                       <  1:147539/217‑1 (MQ=255)                                                                                                                                                             cTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGACTCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACaat                                                 >  1:320986/1‑250 (MQ=255)                                                                                                                                                                                                           gCTACTGGTTACCGGCGCGCCGGTCGAATCTTCGCTTTTTAGCGTCGAATTACTGACCGGCAGAAAGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGCTACGAACTTTGGTGTTGTTTGACGGGTTAAGACGGt  >  1:152721/1‑251 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                 GCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGT  >  NC_000913/664278‑664730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑