Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	631,633	G→A	W580* (TGG→TGA)	cstA →	pyruvate transporter CstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	631,633	0	G	A	100.0%	33.0 / NA	13	W580* (TGG→TGA)	cstA	pyruvate transporter CstA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/8);  total (5/8)

CCGATTCACTGCCTGCTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGT  >  NC_000913/631428‑631863                                                                                                                                                                                                              |                                                                                                                                                                                                                                       ccGATTCACTGCCTGCTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGAcc                                                                                                                                                                                           >  1:5706/1‑251 (MQ=255)   gATTCACTGCCTGCTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTggn                                                                                                                                                                                                               <  1:83347/229‑2 (MQ=255)                       gctgGCAACAGCGCTGGGCGTACGGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGGTGAGGCTCTGTGCCGGGGTGTTGGTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGttt                                                                                                                                                                      <  1:177257/250‑1 (MQ=255)                                                           cTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGGGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGGTGGCGCTGATGCTATGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTa                                                                                                                                                                                                   >  1:120468/1‑185 (MQ=255)                                                                                                              gCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCGCGGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGgc                                                                                                                                                                                                                                   <  1:242912/102‑1 (MQ=255)                                                                                                              gCCGCNNTTNNNTANNGCCAACCAGATGCTGGCAGGGATNGCGCTGATGCTCTGTGCCGTGGTGTTGTTNANGNTGAANNGTCNACGTTACGCCNGAGTGgc                                                                                                                                                                                                                                   >  2:242912/1‑102 (MQ=255)                                                                                                                        ggTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTgg                                                                                                                                          <  1:51833/181‑1 (MQ=255)                                                                                                                                                           gATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAACCGTCTGGATGcc                                  <  1:227353/250‑1 (MQ=255)                                                                                                                                                                  tgtgCCGTGGTGTTGTTCAAGATGAANCGACAANNTTNCGCCTGAGTGGCGCTGGTANCANNNGNNNGNCTGCTNAANNGTACCCTGANCGCAGGCTGGCCGAAAGCGTTTAGCCCGGATGCGAAAGTCgg                                                                                                                                                  >  2:77590/1‑131 (MQ=255)                                                                                                                                                                  tgtgCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCgg                                                                                                                                                  <  1:77590/131‑1 (MQ=255)                                                                                                                                                                                gttCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGAACCAACGGACTGGCTGCTGCTTTGTACCCTGACCGCAGGCTGGCAGAACGCGTTTCGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGAAATATTCCGTCGCAGTATCCTGAGTCACAGCTGGCGCACCTGGTGTTCAACACCCGTCTGGATGCCGGGTTAACCATCTTCTTTAtgg            >  1:136369/1‑251 (MQ=255)                                                                                                                                                                                  tNAANATGAANCGTCNACGTTACGCCTGAGTNGCGCTGGTACCAACGGCCCNNCNGCTGANTTGTa                                                                                                                                                                                                   <  2:120468/66‑1 (MQ=255)                                                                                                                                                                                          aaaCGTCAACGTTACGCCTGAGTGGCGCTGGGACCAACGGCCTGGCGGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCGCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAAGAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGGCGCAGTAGACTGAGTCACAGCTGGCGCAACGGGTGTGCAACAACCGTCGGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCgtggt  <  1:183041/251‑1 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                                                                                                       CCGATTCACTGCCTGCTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGT  >  NC_000913/631428‑631863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑