Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,512,213 C→T A68T (GCG→ACG)  fecD ← ferric citrate ABC transporter membrane subunit FecD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,512,2130CT93.8% 37.1 / ‑3.7 18A68T (GCG→ACG) fecDferric citrate ABC transporter membrane subunit FecD
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (10/5);  total (12/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

GGTGAGCGCCAGCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACA  >  NC_000913/4511992‑4512429
                                                                                                                                                                                                                             |                                                                                                                                                                                                                        
ggTGAGCGCCATCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGTCACGGCGAGGGCTGCACCGACAAACAGTg                                                                                                                                                                                             <  1:11551/251‑1 (MQ=255)
                      tggtggGTCTTGTCCAGCATCTGCAGCAATATCACCCCCGCCATGCCGCCCGCAAAGTCCAGCAGCGGCATCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGTCCAGGCTGGCGGCATTGTTAACACCGAGAATATCCGGTGAGGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGTCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTc                                                                                                                                                                        <  1:220312/250‑1 (MQ=255)
                                                                          cAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGTCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGTCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGGACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTcagc                                                                                                                   <  1:117046/251‑1 (MQ=255)
                                                                                 cagcagCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGTCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAACAAGCGCGGCAGTCGGTACTCCATCAATTCATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCagcagc                                                                                                                <  1:286364/246‑1 (MQ=255)
                                                                                            gcaCCATCACGGGCAGTGACGGCGTAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGTCACAGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAg                                                                                                                                                                                  >  1:298281/1‑170 (MQ=255)
                                                                                                       ggCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGTCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGTAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTcc                                                                                      >  1:48530/1‑251 (MQ=255)
                                                                                                               nggCATAAGAAGTAGAGCCCCCACAGAGGCCCGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCAAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGTCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGACACGGCACGGGGATCACTCCCATATgg                                                                               >  1:37595/2‑250 (MQ=255)
                                                                                                                       gaagTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGGTGCGCAGAATCCCCTGTATCAGCACGCCCGTCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCCTATGGAGTGATAAc                                                                      >  1:230377/1‑251 (MQ=255)
                                                                                                                           tAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGTCACGGCGAGGGCTGCACCGACAAACAGTg                                                                                                                                                                                             <  1:189078/128‑1 (MQ=255)
                                                                                                                                           nccAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGTCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGc                                                  >  1:94342/2‑251 (MQ=255)
                                                                                                                                           gCCAGGCTGGCGGCATGGTTAACAACGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCAGGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGAGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGc                                                  >  1:207559/1‑251 (MQ=255)
                                                                                                                                             cAGGCTGGCGGCATGGTTAACACCGAGATTATCCGGTGATGCCAGAGGGTTGCGCACTATCCCCTGTATCAGCACGTCCGTCCCGGCGAGGGCTGCACCGACAAACAGTTCCAGGAGTAAGCGCAGCAGTCGGTACTCCATCCAAACATAATAATGGTCGTGTCCgacc                                                                                                                                  >  1:146443/1‑166 (MQ=255)
                                                                                                                                             cAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCACCTGTATCAGCACGCCCGTCACGGCGCGGGCCGCACCGACAAACAGTGCCAGCAGCAAg                                                                                                                                                                                  >  1:148892/1‑121 (MQ=255)
                                                                                                                                                   ggcggcATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAACACGCCCGTCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCCAGCGCGGCAGTCGGTACTCCATCACTACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGtg                                           >  1:157268/1‑250 (MQ=255)
                                                                                                                                                                   ccGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCACGTCACGGCGAGGGCTGCACCGACAAAAAGTGCCAGCAGAAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTcagcag                                                                                                                 >  1:211190/1‑164 (MQ=255)
                                                                                                                                                                             ccGGTGATGCCAGAGGGGTGCGCACAATCCCCTGTATCAGCACCCCCGGCACGGCGAGGGCTGCAACGACAAACAGTGCCAGCAGCAAGCGCGGTAGTCGGTGCTCCATCAATACATAATAATGCTCGTCTCCGGCCTGCCAGTCGGTCAGCAGCGCTCGCCACGGCACGGGGATCACTCCCAGATGGAGTGATAACAGCGCACAGCCCTCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATc                <  1:315191/251‑1 (MQ=255)
                                                                                                                                                                                             gTTGCGCACAATCCCCTGTATCAGCACGCCCGACACCGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGTCAAGGATGCTGAAAATAACCAGAGCAATTTTCATCCTCGCCTCCTcaca  >  1:118004/1‑249 (MQ=255)
                                                                                                                                                                                                                 tCAGCACGCCCGTCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTc                   >  1:15444/1‑212 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                        
GGTGAGCGCCAGCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACA  >  NC_000913/4511992‑4512429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: