Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,406,491 C→T A101V (GCC→GTC)  nsrR → DNA‑binding transcriptional regulator NsrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,406,4910CT80.0% 11.1 / ‑0.4 10A101V (GCC→GTC) nsrRDNA‑binding transcriptional regulator NsrR
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (5/3);  total (7/3)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01

CTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAACCGGCGAGTGCGATACGTATTGGTGATGTGGTGCGCGAGCTGGAGCCCTT‑ATCGCTGGTGAATTGCAGCAGTGAGTTTTGCCACATTACACCTGCCTGTAGGTTGAAACAGGCACTTTCTAAGGCCGTGCAAAGTTTTCTTACGGAACTGGATAACTACACGCTTGCCGATTTGGTTGAAGAGAATCAACCGCTTTATAAATTATTGCTGGTGGAGTGACGAAAATCTTCATCAGAGATGACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACC  >  NC_000913/4406291‑4406740
                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         
cTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGAAAACCGGCGAGTGCGATACGTATTGGTGATGTGGTGCGCGAGCTGGAGCCCTT‑ATCGCTGGTGAATTGCAGCAGTGAGTTTTGCCACATTACACCTGTCTGTAGGTTGAAACAGGCACTTTCTaa                                                                                                                                                                                                                                >  1:69616/1‑228 (MQ=255)
                                                  gNGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAACCGGCGAGTGCGATACGGATTGGTGATGTGGTGCGCGAGCTGGAGCCCTT‑ATCGCTGGTGAATTGCAGCAGAGAGTTTTGCCACATTACACCTGCCTGTAGGTTGAAACAGGc                                                                                                                                                                                                                                         >  1:254460/1‑169 (MQ=255)
                                                         cTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAACCGGCGAGAGCGATAAGTATTGGTGATGTGGGGCGCGAGGTGGAGCCCTT‑ATCGCTGGTGAATTGCAGCAGTGAGTTTTGCCACATTACACCTGTCTGTAGGTTGAAACAg                                                                                                                                                                                                                                           >  1:295726/1‑160 (MQ=255)
                                                                                                    ggTAAACCGGCGAGTGCGATACGTATTGGTGATGTGGTGCGCGAGCTGGCGCCCTTTCTCGCTGGCGAATTGCCGCAGTGAGTTTTGCCACATTACACCTGTCTGTAGGTTGAAACAGGCACTTTCTAAGGCCGTGCAAAGTTTTCTTACGGAACTGGATAACTACACGCTTGCCGATTTGGTTGAAGAGAATCAACCGCTTTATCAATTATTGCTGGTGGAGTGACGAAAATCtt                                                                                                                     >  1:212373/1‑236 (MQ=255)
                                                                                                                         cGTATTGGTGATGTGGTGCGCGAGCTGGAGCCCTT‑ATCGCTGGTGAATTGCAGCAGTGAGTTTTGCCACATTACACCTGTCTGTAGGTTGAAACAGGCACTTTCTAAGGCCGTGCAAAGTTTTCTTACgg                                                                                                                                                                                                         <  1:14232/130‑1 (MQ=255)
                                                                                                                               ggggATGTGGTGCACGAGCTGGAGCCCTT‑ATCGCTGGTGAATTGCAGCAGTGAGTTTTGCCACATTACACCTGTCTGTAGGTTGAAACAGGCACTTTCTAATGCCGTGCAAAGTTTTCTTACGGAACTGGATAACTACACGCTTGCCGATTTTGTTGAAGAGAATCAACCGCTTAATAAATTATAGCTGGGGGAGTGACGAAAATCTTCATCAGAGATGACAACGGAGGAACCGAGATGTCACAAGATcn                                                                           <  1:28289/247‑2 (MQ=255)
                                                                                                                                gTGATGTGGTGCGCGAGCTGGAGCCCTT‑ATCGCTGGTGAATTGCAGCAGTGAGTTTTGCCACATTACCCCTGTCTGTAGGTTGAAAc                                                                                                                                                                                                                                             >  1:140913/1‑87 (MQ=255)
                                                                                                                                        gtgCGCGAGCTGGAGCCCTT‑ATCGCTGGTGAATTGCAGCAGTGAGTTTTGCCACATTACACCTGCCTGTAGGTTGAAACAGGCACTTTCTAAGGCCGTGCAAAGTTTTCTTACGGAACTGGATAACTACACGCTTGCCGATTTGGTTGAAGAGAATCAACCGCTTTATAAATTATTGCTGGTGGAGTGACGAAAATCTTCATCAGAGATGACAACggag                                                                                                 >  1:50541/1‑219 (MQ=255)
                                                                                                                                                     aNCCNTT‑ATCNCTGGNGAATTGCAGCAGTGANTTTTGCCACATTACACCTGNNTNTAGGTNGAAAc                                                                                                                                                                                                                                             <  2:140913/66‑1 (MQ=255)
                                                                                                                                                     aGCCCTT‑ATCGCTGGTGAATTGCAGCAGTGAGTTTTGCCACATTACACCTGTCTGTAGGTTGAAACAGGCTCTTTCTAAGGCCGTGCAAAGTTTTCTTACgn                                                                                                                                                                                                         <  1:32304/102‑2 (MQ=255)
                                                                                                                                                                                              caTTACACCTGTCTGTAGGTTGAAACAGGCACTTTCTAAGGCCGTGCACAGTTTTATTACGGAACTGGATAACTACACGCTTGCCGATTTGGTTGAAGAGAATCAACCGCTTTATAAATTATTGCTGGTGGAGTGACGAAAATCTTCACCAGAGATGACAACGGAGGAACCGAGATGTCACAAGATc                                                                            >  1:317066/1‑187 (MQ=255)
                                                                                                                                                                                                        gcctgTAGGTTGAAACAGGCACTTTCTAAGGCCGTGCAAAGTTTTCTTACGGAACTGGATAACTACACGCTTGCCGATTTGGTTGCAGAGAATCAACCGCTTTATAAATTATTGCTGGTGGAGTGACGAAAATCTTCCTCAGAGATGACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCACGAACATTTAAcc  >  1:157513/1‑251 (MQ=255)
                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         
CTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAACCGGCGAGTGCGATACGTATTGGTGATGTGGTGCGCGAGCTGGAGCCCTT‑ATCGCTGGTGAATTGCAGCAGTGAGTTTTGCCACATTACACCTGCCTGTAGGTTGAAACAGGCACTTTCTAAGGCCGTGCAAAGTTTTCTTACGGAACTGGATAACTACACGCTTGCCGATTTGGTTGAAGAGAATCAACCGCTTTATAAATTATTGCTGGTGGAGTGACGAAAATCTTCATCAGAGATGACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACC  >  NC_000913/4406291‑4406740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑