Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,045	G→A	D256N (GAC→AAC)	metA →	homoserine O‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,045	0	G	A	92.3%	29.4 / ‑4.4	14	D256N (GAC→AAC)	metA	homoserine O‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (7/5);  total (8/6)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGAC‑TAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCA  >  NC_000913/4214823‑4215258                                                                                                                                                                                                                                |                                                                                                                                                                                                                      ggCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGAC‑TAAACCCGGATGTACCGTATAACTATTTcc                                                                                                                                                                                            <  1:114734/250‑1 (MQ=255)                  ncTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGAC‑TAAACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCaa                                                                                                                                                                           >  1:36611/2‑250 (MQ=255)                                   gcTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGCATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGAC‑TAAACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCgagcgagc                                                                                                                                                         >  1:250349/1‑251 (MQ=255)                                                                        nacCGATCTGGAAATTCTGGCCGAGACGGCAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGAC‑TAAACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGc                                                                                                                    >  1:261532/2‑251 (MQ=255)                                                                                                                     tCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGAC‑TAAACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACATCTACCAGATCACGCCATACGATCTACGGcac                                                                        >  1:124201/1‑250 (MQ=255)                                                                                                                            gCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGAC‑TAAACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCAGACGATCTACGGCACATGAATc                                                                 <  1:252186/250‑1 (MQ=255)                                                                                                                                              aTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCACACGCTGGCGAAGGAACTTATCAGCGCTGTGGAAGCCG‑ACATCCACCCGGATGTACTGTATAACTATTTCCAGCACAATGATCCGCAAAATACACCGCGCACGAGCTGGCGAAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGACTCCAACGCTGGCTTAAtcttc                                              >  1:160533/1‑251 (MQ=255)                                                                                                                                                            gggCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGAC‑TAAACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTAcc                                                                                                  <  1:160713/185‑1 (MQ=255)                                                                                                                                                                cATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGAC‑TAAACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACAGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGtt                             >  1:174228/1‑250 (MQ=255)                                                                                                                                                                                  cAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGAC‑TAGACCCGGATGTACCGTATAGCTAGTTCCCGCACAATGAGCCGCAAAATACGCCGCGAGCGAGCTGTCGTAGTCACGGTAATTTACTGGTGGCCAACTGGCTCAACTATTACGTGTACCAGAGCACGCAATACGATCTACGGCACATGAATCCaan                                                             <  1:29632/200‑2 (MQ=255)                                                                                                                                                                                              cAGGAATTTTTCCGCGATGTGGAAGCCGGAC‑TAAACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTTCCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCa  >  1:318659/1‑247 (MQ=255)                                                                                                                                                                                                         ccGCGATGTTGAAGCCGGAC‑TAAACCCGGATGTACCGTATAACTATTGCCCGCACAATGATCCGCAAAATACACCGCTAGCGAGCTGGCGTAGTCACGGTAATTTACTTTTTACCAACTGGCTCGACTAGTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAAtc                                                 <  1:176248/189‑1 (MQ=255)                                                                                                                                                                                                                  ggAAGCCGGAC‑TAAACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAAttt                                                                                                                                     <  1:275430/96‑1 (MQ=255)                                                                                                                                                                                                                       ccGGAC‑TAAACACGGATGTACCGTATAACTATTTCCCGCCCAATGATCCGCACAATACACCGCGAGCGAGCTGGCGAAGTCACGGTAATTTACTGTTTACCAACTgg                                                                                                                     >  1:116199/1‑107 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                      GGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGAC‑TAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCA  >  NC_000913/4214823‑4215258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑