Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,014,729	C→T	L559L (CTC→CTT)	metE →	cobalamin‑independent homocysteine transmethylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,014,729	0	C	T	100.0%	14.5 / NA	7	L559L (CTC→CTT)	metE	cobalamin‑independent homocysteine transmethylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/4);  total (3/4)

GGAATACTTTGGCGAGCACCTCGACGGATTTGTCTTTACGCAAAACGGTTGGGTACAGAGCTACGGTTCCCGCTGCGTGAAGCCACCGATTGTCATTGGTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACAC  >  NC_000913/4014525‑4014959                                                                                                                                                                                                             |                                                                                                                                                                                                                                       ggAATCCTTTGGCGAGCACCTCGACGGATTTGTCTTTACGCAAAACGGTTGGGTACAGAGCTACGGTTCCCGCTGCGTGAAGCCACCGATTGTCATTGGTGACATTAGCCGCCCGGCATCGATTACCTTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGTGCCGGTGACCATACTTTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGcc                                                                                                                                                                                           <  1:245361/250‑1 (MQ=255)                      ngACGGATTTGTCTTTACGCACAACGGTTGGGTACAGAGCTACGGTTCCCGCTGCGTGAAGCCACCGATTGTCATTGGTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTTTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGc                                                                                                                                                                     >  1:78266/2‑251 (MQ=255)                       gACGGATTTGTCTTTACGCAAAACGGTTGGGTACAGAGCTACGGTTCCCGCTGCGTGAAGCCACCGATTGTCATTGGTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTTTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCACACAGATTGCGCTGGCGCTGCg                                                                                                                                                                    >  1:169678/1‑251 (MQ=255)                                                                                                                             aCCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTTTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGAGGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCtn                                                                                  <  1:63595/231‑2 (MQ=255)                                                                                                                                     gTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTTTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCTTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAgg                                                                                            <  1:273797/213‑1 (MQ=255)                                                                                                                                                                          gTGAAAGGGATGCTGACGGGGCCGGTGACCATACTTTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGt                 >  1:185753/1‑251 (MQ=255)                                                                                                                                                                                                       cATACTTTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGAGGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAAGCGGCGCTGCGCGAAGTTTTACCGCTGCTTCGTAGCGACGGGGATGCGGATCTCCAGTGGGGCGTAGAGGCCGTCCGTATCAACGCCGCCGTGGCGAAAGATGacac  <  1:127940/237‑1 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                                                                       GGAATACTTTGGCGAGCACCTCGACGGATTTGTCTTTACGCAAAACGGTTGGGTACAGAGCTACGGTTCCCGCTGCGTGAAGCCACCGATTGTCATTGGTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACAC  >  NC_000913/4014525‑4014959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑