Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,893,219	G→A	G152D (GGT→GAT)	yidZ →	DNA‑binding transcriptional dual regulator YidZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,893,219	0	G	A	100.0%	12.8 / NA	6	G152D (GGT→GAT)	yidZ	DNA‑binding transcriptional dual regulator YidZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/3);  total (3/3)

CCACCAGACACCGCGCGGCCTGAAGTTTGAGCTGGCGGCGGAATCACCGCTGATGATGATCATGCTTAATGCGCTGTCGAAACAGATCTACCAACGTTACCCGCAGGCGACCATCAAATTACGTAACTGGGATTACGATTCCTTAGATGCCATTACTCGTGGTGAAGTGGATATCGGTTTTTCCGGTCGCGAAAGCCATCCTCGCTCGCGGGAGCTGTTAAGCTCGCTACCGTTAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGCGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGG  >  NC_000913/3893034‑3893440                                                                                                                                                                                          |                                                                                                                                                                                                                              ccaccaGACACCGCGCGGCCTGAAGTTTGAGCTGGCGGCGGAATCACCGCTGATGATGATCCTGCTTAATGCGCTGTAGAAACAGATCTACCAACGTTACCCGCAGGCGACCATCAAATTACGTCACTGGGATTACGATTCCTTAGATGCCATTACTCGTGGTGAAGTGGATATCGGTTTTTCCGATCGCGAAAGCCATCCTCGCTCGCGGGAGCTGTTAAGCTCGCTACCGTTAGCCATTGATTATGa                                                                                                                                                                >  1:298238/1‑249 (MQ=255)                                               ccGCTGATGATGATCATGCTTAATGCGCTGTCGAAACAGATCTACCAACGTTACCCGCAGGCGACCATCAAATTACGTAACTGGGATTACGATTCCTTAGATGCCATTACTCGTGGTGAAGTGGATATCGGTTTTTCCGATCGCGAAAGCCATCCTCGCTCGCGGGAGCTGTTAAGCTCGCTACCGTTAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGatcatcn                                                                                                                <  1:84725/251‑2 (MQ=255)                                                                                        cTACCAACGTTACCCGCAGGCGACCATCAAATTACGTAACTGGGATTACGATTCCTTAGATGCCATTACTCGTGGTGAAGTGGATATCGGTTTTTCCGATCGCGAAAGCCATCCTCGCTCGCGGGAGCTGTTAAGCTCGCTACCGGTAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGc                                                                                                             <  1:123500/213‑1 (MQ=255)                                                                                                    cccGCAGGCGACCATCAAATTACGTAACTGGGATTACGATTCCTTAGATGCCATTACTCGTGGTGAAGTGGATATCGGTTTTTCCGATCGCGAAAGCCATCCTCGCTCGCGGGAGCTGTTAAGCTCGCTACCGTTAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATc                                                            >  1:13814/1‑250 (MQ=255)                                                                                                       gCAGGCGACCATCAAATTACGTAACTGGGATTACGATTCCTTAGATGCCATTACTCGTGGTGAAGTGGATATCGGGTTTTCCGATCGCGAAAGCCGTCCTCGCTCGCGGGAGCTGTTAAGCTCGCTACCGTTAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGc                                                                                                                                            <  1:265064/167‑1 (MQ=255)                                                                                                                                                             tCGTGGTGAAGTGGATATCGGTTTTTCCGATCGCGAAAGACCTCCTCGCTCGCGGGAGCTGTTAAGCTCGCTACCGTTAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGCCCTGTGTCTGGTTACGCAAAGATCATCCGGCACTGTCTCAAACGTGGAATTTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGCGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTgg  >  1:327083/1‑251 (MQ=255)                                                                                                                                                                                          |                                                                                                                                                                                                                              CCACCAGACACCGCGCGGCCTGAAGTTTGAGCTGGCGGCGGAATCACCGCTGATGATGATCATGCTTAATGCGCTGTCGAAACAGATCTACCAACGTTACCCGCAGGCGACCATCAAATTACGTAACTGGGATTACGATTCCTTAGATGCCATTACTCGTGGTGAAGTGGATATCGGTTTTTCCGGTCGCGAAAGCCATCCTCGCTCGCGGGAGCTGTTAAGCTCGCTACCGTTAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGCGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGG  >  NC_000913/3893034‑3893440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑