Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	511,499	C→T	intergenic (‑120/‑142)	copA ← / → glsA	Cu(+) exporting P‑type ATPase/glutaminase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	511,499	0	C	T	84.6%	20.1 / ‑2.7	13	intergenic (‑120/‑142)	copA/glsA	Cu(+) exporting P‑type ATPase/glutaminase 1
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (5/6);  total (6/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

CTTCAGTGATAGACACATCCGCCTGCTCAACATCCGGACGCTGTTCAAGACTTTCTTTCACGCGTTTAACGCAGTGACCGCAGGACAGGCCGTCCAGGGTCAGGTCGATAGTTTGTGACATAAAACACTCCTTTAAGACAGTTTTGACTGGCTGTGATAAAGGTTAAACCTTCCAGCAAGGGGAAGGTCAAGAAATTAATAAACCAGGCGGGTAAAAGTCCGTAAAGATTAAAAAATCGGCTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCTTACACCCAATTTC  >  NC_000913/511259‑511695                                                                                                                                                                                                                                                 |                                                                                                                                                                                                     cTTCAGTTATAGACACATCCGCCTGCTCAACATCCGGAAGATGTTCAAGAATTTCTTTAACGCGTTTAACGCAGTGCCGGCAGGACAGGCAGTCATGGGACAGGTCGATAGTTTGTGACATAAAACACTCCTTTAAGACGGTTTTGACTGGCTGTGATAAAGGTTAAACCTTCCAGCAAGGGGAAGGTCAGGAACTTAATAAACCAGGCGGGTCAAAGTCCGGCAAGATTATCAGATCGGTTCGATTTg                                                                                                                                                                                              >  1:230352/1‑249 (MQ=255)                                                  aCTTTCTTTCACGCGTTTAACGCAGTGACCGCAGGACAGGCCGTCCAGGGTCAGGTCGATAGTTTGTGACATAATACACTCCTTTAAGACAGTTTTGACTGGCTGTGATAAAGGTTAAACCTTCCAGCAAGGGGAAGGTCAAGAAATTAATAAACCAGGCGGGTAAAAGTCCGTAAAGATTAAAAAATCGGTTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGt                                                                                                                                           <  1:11357/251‑1 (MQ=255)                                                                                            gTCCAGGGTCAGGTCGATAGTTTGTGACATAAAACACTCCTTTAAGACAGTTTTGACTGGCTGTGATAAAGGTTAAACCTTCCAGCAAGGGGAAGGTCAAGAAATTAATAAACCAGGCGGGCAAAAGTCCGTAAAGATTAAAAAATCGGTTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTTCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTg                                                                                                  >  1:129701/1‑250 (MQ=255)                                                                                            gTCCAGGGTCAGGTCGATAGTTTGTGACATAAAACACTCCTTTAAGACAGTTTTGACTGGCTGCGATAAAGGTTAAACCTTCCAGCAAGGGGAAGGTCAAGAAATTAATAAACCAGGCGGGTAAAAATACGGAAAAATTAAAAAATCAGCTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAATTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTg                                                                                                  >  1:188611/1‑250 (MQ=255)                                                                                                 gggTCAGGTCGATAGTTTGTGACATAAAACACTCCTTTAAGACAGTTTTGACTGGCTGTGATAAAGGTTAAACCTTCCAGCGAGGGGAAGGTCAAGAAATTAATAAACCAGGCGGGTAAAAGTCCGTAAAGATTAAAAAATCGGCTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGTGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAAt                                                                                            <  1:140405/251‑1 (MQ=255)                                                                                                                     gACATAAAACAATCCTTTAAGACAGTTTTGACTGGCTGTGATAAAGGTTAAACCTTCCAGCAAGGGGAAGATCACGAAATTAATAAACCAGGCGGGAAAAAATGCGGAAAGATTAAAAAATGGGTGCGATTTGCATCATGATTAAACATTTATCCCTTTGTTTTCCTGTAGTTAAGCTGGGGGTACTAAGTTAAATCCGGATGCCTGAAAATCGGCACCTGAGTGGGGAACTACCGCCCACATccctaaa                                                                         >  1:160115/1‑244 (MQ=255)                                                                                                                                                    cTGGCTGTGATAAAGGTTAAACCTTCCAGCAAGGGGAAGTTCAAGAAATTAATAAACCAGGCGGGTAAAATACCGTAAAGATTAAAAAGTCCGTGCGATTTGTATCAGGATAAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGt                                                                                                                                           <  1:300019/153‑1 (MQ=255)                                                                                                                                                             aTAAAGGTTAAACCTTCCAGCAAGGGGAAGGTCAAGAAATTAATTAACCAGGCGGGTAAAAGTCCGTAAAGATTAAAAAATCGGTTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGGTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATGACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTAcagn                                <  1:36437/251‑2 (MQ=255)                                                                                                                                                             aTAAAGGTTAAACCTTCCAGCAAGGGGAAGGTCAAGAAATTAATAAACCAGGCGGGGAAAAGCCCGTAAAGATTAAAAAATCGGTTCGATTTGCCTCAGGATTAGACATTTATCTCTTTGTTTTCCTGGAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGTAAATCGGCACCGGGGTGGGGAATTACCTCCCGCATCTCTGAAAAGGAGTTAACAAAAGATTTTAGATGCCAACAACTTAcagc                                >  1:141581/1‑251 (MQ=255)                                                                                                                                                                               aGCAAGGGGAAGGTCAAGAAATTAATAAACCAGGCGGGTAAAAGTCCGTAAAGATTAAAAAATCGGTTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCAc                                                                                                         <  1:117875/160‑1 (MQ=255)                                                                                                                                                                                                gAAATTAATAAACCAGGCGGGTAAAAGTCCGTAAAGATTAAAAAATCGGTTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTaaa                                                                                                                                >  1:75714/1‑120 (MQ=255)                                                                                                                                                                                                gAAATTAATAAACCAGGCGGGTAAAAGTCCGNAAAGATTAANNAANCGGTTNGNNNTNNNTCNGGATTAGACATTTATCTCTNTGNNTTCCTGNAGTTAAGTTGCGGGTGCTAAGTTaaa                                                                                                                                <  2:75714/120‑1 (MQ=255)                                                                                                                                                                                                                                        aaaaaTCGGTTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCTTACACCCAATTtc  <  1:26981/206‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                     CTTCAGTGATAGACACATCCGCCTGCTCAACATCCGGACGCTGTTCAAGACTTTCTTTCACGCGTTTAACGCAGTGACCGCAGGACAGGCCGTCCAGGGTCAGGTCGATAGTTTGTGACATAAAACACTCCTTTAAGACAGTTTTGACTGGCTGTGATAAAGGTTAAACCTTCCAGCAAGGGGAAGGTCAAGAAATTAATAAACCAGGCGGGTAAAAGTCCGTAAAGATTAAAAAATCGGCTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCTTACACCCAATTTC  >  NC_000913/511259‑511695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑