Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,810,916	G→A	R79R (CGC→CGT)	mutM ←	DNA‑formamidopyrimidine glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,810,916	0	G	A	81.8%	13.7 / ‑0.6	12	R79R (CGC→CGT)	mutM	DNA‑formamidopyrimidine glycosylase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (3/6);  total (4/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

ATGCCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATCCACATGGTCATGCTTTTCAGGGGGAAGTTCTTCTGGAAGGATGCGCAGGCTGCCAGACATCCCTAAATGAATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCA  >  NC_000913/3810784‑3811132                                                                                                                                     |                                                                                                                                                                                                                         aTGCCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATCCACATGGTCATGCTTTTCAGTGGGAAGTTCTTCTGGAAGGATACGCAGGCTGCCAGACATcc                                                                                                                                                                                                       <  1:153024/152‑1 (MQ=255)                   ggTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATCCACATGGTCATTCTTTTCAGGGGGAAGTTCTTCTGGAAGGATACGCAGGCTGCCAGACATCCCTAAATGGATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACTGTAGATCTCTTCTGAATCCGGCCAg                                                                                   <  1:75858/250‑1 (MQ=255)                            ccAGGCACCAAAGCGGCGCGGCTCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATACACATGGTCATGCTTTTCAGGGGGAAGTTCTTCTGGAAGGATACGCAGGCTGc                                                                                                                                                                                                                >  1:292489/1‑116 (MQ=255)                                gCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTTCCGGTGCTCAGCTCCAATTCCACATGGCCATGCGGTTCAGGTGTAAGTTCTGCTGCATTGATTCGCAGGCTTCCAGACATCCCTAAATGAATGATAATCCAGCCCTCAGGCa                                                                                                                                                                         <  1:160980/151‑1 (MQ=255)                                    cAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATCCACATGGTCATGCTTTTCAGGTGGAAGTTCTTCTGGAAGTATACGCAGGCTGCCAGACACCCCTAAAGGAATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCGGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTgcgc                                                                 <  1:56411/251‑1 (MQ=255)                                            ggcgGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATCCACAGGGTCATGCTTTTCAGGGGGAAGTTCTTCTGGAAGGATACGCAGGCTGCCAGACATCCCTAAATGAATGATAATCCAGCCCTCAGTCAGCTCCATCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACTgn                                                         <  1:66785/250‑2 (MQ=255)                                            cgcgGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACAAAATCCACATGGTCATGCTTTTCAGGGGGAAGTTCTTCTGGAAGGATACGCAGGCTGCCAGACATCCCTCAATGAATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTgcgc                                                                 >  1:243718/1‑243 (MQ=255)                                                    ggTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATCCACATGGTCATGCTTTTCAGGAGGAAGTTCTTCTGGAAGGATGCGCAGGCTGCCAGACATCCCTAAATGAATGATAATCCAGCCCTCAGGCAGCNCcannnnnag                                                                                                                                                            >  2:195538/1‑144 (MQ=255)                                                         aGCGCAGCACTTTGCCGTTGCTCATCACCAAATCCACATGGTCATGCTTTTCAGGGGGAAGTTCTTCTGGAAGGATACGCAGGCTGCCAGACATCCCTAAATGAATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACTGCATGAAGAATGGtt                                            >  1:21041/1‑251 (MQ=255)                                                                 aCTTTGCCGTTGCTCATCACCAAATCCACATGGTCATGCTTTTCAGGAGGAAGTTCTTCTGGAAGGATGCGCAGGCTGCCAGACATCCCTAAATGAATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACTGCATGAAGAATGGTTGCACCaan                                    <  1:195538/251‑2 (MQ=255)                                                                         gTTGCTCATCACCAAATCCACATGGTCATGCTTTTCAGGGGGAAGTTCTTCTGGAAGGATACGCAGGCTGCCAGACATCCCTAAATGAATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACTGCATGAAGAATGGTTGCACCAACGAGATGc                             <  1:104313/250‑1 (MQ=255)                                                                                                   cATGCTTTTCAGTGGAAAGTTCTTCTGGAAGGATACGCAGGCTGCGAGACAGCCCTAAAGGAATGATAATCCATCCCGCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGATACCGGCCAGCGCAAGCGTCCGTTGCGCACCTCTGCATGAAGAATGGTTGCACCAACGAGATGCTGTTCTATGCCGCGGCGGCGGGTTTCa  <  1:287604/251‑1 (MQ=255)                                                                                                                                     |                                                                                                                                                                                                                         ATGCCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATCCACATGGTCATGCTTTTCAGGGGGAAGTTCTTCTGGAAGGATGCGCAGGCTGCCAGACATCCCTAAATGAATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCA  >  NC_000913/3810784‑3811132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑