Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	475,410	C→T	A11V (GCG→GTG)	ybaY →	PF09619 family lipoprotein YbaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	475,410	0	C	T	100.0%	23.0 / NA	10	A11V (GCG→GTG)	ybaY	PF09619 family lipoprotein YbaY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/7);  total (3/7)

CAGCAAGCCAAAGTGAGTTGAGTATAACGCAAATTTGCTACTGGTCCGATGGGTGCAATGGTCTGAATTACGGGCTAATTACAGGCAGAAATGCGTGATGTGTGCCACACTTGTTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGAAAGTTAGCCCGCTGGTGCATTGATAATAAGGAGAAATGAATGAAACTCGTGCACATGGCCAGTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCACCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACAC  >  NC_000913/475188‑475592                                                                                                                                                                                                                               |                                                                                                                                                                                       cagcaAGCCAAAGTGAGTTGAGTATAACGCAAATTTGCTACTGGTCCGATGGGTGCAATGGTCTGAATTACGGGCTAATTACAGGCAGAAATGCGTGATGTGTGCCACACTTGTTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGAAAGTTAGCCCGCTGGTGCATTGATAATAAGGAGAAATGAATGAAACTCGTGCACATGGCCAGTGGTTTAGTGGTTGCGATTGCGTTGGCGGCTTgcgc                                                                                                                                                             >  1:13102/1‑250 (MQ=255)                                                                                           aTGCGTGATGTGTGCCACACTTGTTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGAAAGTTAGCCCGCTGGTGCATTGATAATAAGGAGAAATGAATGAAACTCTTGCACATGGCCAGTGGTTTAGTGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCgg                                                                                                                                               <  1:243561/174‑1 (MQ=255)                                                                                           aTGCGNNATNNNTGNNACACTTGTTGACGTTACTATTTTNTTAACCACTCTTCCGGCGAGGAAAGTTAGNCNGNTGGTNNATTNATAATAAGGANAAATGAATGAAACTCGTGCACATGGCCAGTGGTTTAGTGGTTGCGATTNCNTTGGCGGCTTGCGCCGATAAAAGCGCgg                                                                                                                                               >  2:243561/1‑174 (MQ=255)                                                                                                          cacaCTTGTTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGAAAGTTAGCCCGCTGGTGCATTGATAATAAGGAGAAATGAATGAAACTCGTGCACATGGCCAGTGGTTTAGTGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCACCGGCTGCAAATACGGCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCTgg                                                   <  1:127277/251‑1 (MQ=255)                                                                                                                                       ccACTCTTCCGGCGAGGAAAGTTAGCCCGCTGGTGCATTGATAATAAGGAGAAATGAATGAAACTCGTGCACATGGCCAGTGGTTTAGTGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCACCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTgcc                            <  1:22621/245‑1 (MQ=255)                                                                                                                                              tCCGGCGAGGAAAGTTAGCCCGCTGGNGCATTGNNAANAAGGAGAAATGAATGAAACNCGNNNANNNGNCCAGTNGTNNAGTGGTTGCNATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCCGCACCNGCTGCAAATNNGTCTATTTCAGCAACACAACANCCCNNTNNCNAGCNAc                                                                          >  2:72431/1‑192 (MQ=255)                                                                                                                                              gccGGCGAGGAAAGTTAGCCCGCTGGTGCATTGATAATAAGGAGAAATGAATGAAACTCGTGCACATGGCCAGTGGTTTAGTGGTTGCGATTGCGGTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCACCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCGGGTACCGTCt                                                     <  1:72431/212‑1 (MQ=255)                                                                                                                                                        aaaGTTAGCCCGCTGGTGCATTGATAATAAGGAGAAATGAATGAAACTCGTGCACATGGCCAGTGGTTTAGTGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCACCGGCTGCAAATACGTCTATTTCAGcaac                                                                                                 <  1:242527/159‑1 (MQ=255)                                                                                                                                                        aaaGTNNGCNNNCTNNGGCATTGATAATAAGGAGAAATGNATGAAACTCGTGCACATGGCCAGTGGTTTNGNGNTTGCNNTTGNGTTGGCGGCTNGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCACCGGCTGCAAATANGNCTATTTCAGcaac                                                                                                 >  2:242527/1‑159 (MQ=255)                                                                                                                                                           gTTAGCCCGCTGGTGCATTGATAATAAGGAGAAATGAATGAAACTCGTGCACATGGCCAGTGGTTTAGTGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCACCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGacac  <  1:181612/251‑1 (MQ=255)                                                                                                                                                                     tGGTGCATTGATAATAAGGAGAAATGAATGAAACTCGTGCACATGGCCAGTGGTTTAGTGGTTGCGATTGCGTTggcn                                                                                                                                                                     <  1:63224/78‑2 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                       CAGCAAGCCAAAGTGAGTTGAGTATAACGCAAATTTGCTACTGGTCCGATGGGTGCAATGGTCTGAATTACGGGCTAATTACAGGCAGAAATGCGTGATGTGTGCCACACTTGTTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGAAAGTTAGCCCGCTGGTGCATTGATAATAAGGAGAAATGAATGAAACTCGTGCACATGGCCAGTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCACCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACAC  >  NC_000913/475188‑475592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑