Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,908,9890GA71.4% 17.5 / 12.2 21G22E (GGA→GAA) yobHuncharacterized protein YobH
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base A (7/8);  total (11/10)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGTTAGTTAAACAGCGCCTAACTATGTCGATATTCGTGCTTTTTCGGAATGAGTCGCTTGCTTCAGCGCATATTGCCGCTACGATTAAGCGAACATAAAAAGAGAAGAGGTTGTAATGCGATTCATCATTCGCACAGTTATGCTGATAGCACTGGTATGGATAGGATTATTACTCAGCGGATATGGCGTTTTGATTGGAAGCAAAGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCCCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATACTCTTATAGCAAGCGCGTTT  >  NC_000913/1908744‑1909223
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          
aCGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGGTAGTTAAACAGCGCCTAACTATGTCGATATTCGTGCTTTTTCGGAATGAGTCGCTTGCTGCAGCGCAGATGTCCGCTACGATTAAGCGAACATAAAAAGAGAAGAGATGGTAATGCGATTCATCATTCGCACAGTTATGCTGATAGCACTGGTATGGATAGGACTATTACTCAGCGGATATg                                                                                                                                                                                                                                       <  1:284012/251‑1 (MQ=255)
                    cACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTCGCATGCGTTGGTTACACAGCGCCTCCCTTCGTCGATATTCGTGGTTTTTCGGAATGAGTCGCTTGCTTCAGCGCATATTGCCGCTACGATTAAGCGAACATAAAAAGAGAAGAGTTTGTAATGCGATTCATCATTCGCACCGTTATGCTGATAGCACTGGTCTGGATAGGATTATTACTAAGCGGATATGGCGTTTTGATTGGAAGCaa                                                                                                                                                                                                                    >  1:148708/1‑250 (MQ=255)
                                      acctacctTATGTCATTAAACTAGAATGCGTTAGTTAAACAGCGCCTAACTATGTCGATATTCGTGCTTTTTCGGAATGAGTCGCTTGCTTCAGCGCATATTGCCGCTACGATTAAGCGAAAATAAAAAGAGACGAGGTTGTAATGCGATTCATCATTCGCACAGTTATGCTGATAGCACTGGTATGGCTAGGATTATTACTCAGCGGATATGGCGTTTTGATTGGa                                                                                                                                                                                                                         >  1:262871/1‑227 (MQ=255)
                                       cctacctTATGTCATTAAAATAGCATGCGTTAGTTAAACAGCGCCTAACTATGTCGATATTCGTGCTTTTTCGGAATGAGTCGCTTGCTTCAGCGCATATTGCCGCTACGATTAAGCGAACATAAAAAGAGAAGAGGTTGTAATGCGATTCATCATTCGCACAGTTATGCTGATAGCACTGGTATGGATAGGATTATTACTCAGCGGata                                                                                                                                                                                                                                         >  1:250944/1‑210 (MQ=255)
                                                   cATTAAACTAGCATGCGTTAGTTAAACAGCGCCTAACTATGTCGATATTCGTGCTTTTTCGGAATGAGTCGCTTGCTGCAGCGCATATTGCCGCTACGATTAAGCGAACATAAAAAGAGAAGAGGTTGTAATGCGATTCATCATTCGCACAGTTATGCTGATAGCACTGGTATGGATAGGATTATTACTCAGCGAATATGGCGTTTTGATTGGAAGCAAAGAGAATGCCGCAGGATTAGGTTTGCAATGt                                                                                                                                                                                     <  1:160255/250‑1 (MQ=255)
                                                                                                               ggAATGAGTCGCTTGCTTCAGCGCATATTGCCGCTACGATTAAGCGAACATAAAAAGAGAAGAGGTTGTAATGCGATTCATCATTCGCACAGTTATGCTGATAGCACTGGTATGGATAGGATTATTACTCAGCGGATATGGc                                                                                                                                                                                                                                     <  1:129967/142‑1 (MQ=255)
                                                                                                                     cgTCGCTTGCTTCAGCGCATATTGCCGCTACGATTAAGCGAACATAAAAAGAGAAGAGGTTGTAATGCGATTCATCATTCGCACAGTTATGCTGATAGCACTGGTATGGATAGGATTATTACTCAGCGAATATNGCGNNNNNAtnnnnnnnnnn                                                                                                                                                                                                                   >  2:191431/2‑144 (MQ=255)
                                                                                                                     aGTCGCTTGCTTCAGCGCATATTGCCGCTACGATTAAGCGAACATAAAAAGAGAAGAGGTTGTAATGCGATTCATCATTCGCACAGTTATGCTGATAGCACTGGTATGGATAGGATTATTACTCAGCGAATATGGCGTTTTGATTGGAAGCAAAGAGAATGCCGCAGGATTAgn                                                                                                                                                                                               <  1:191431/174‑2 (MQ=255)
                                                                                                                              cttCAGCGCATATTGCCGCTACGATTAAGCGAACATAAAAAGAGAAGAGGTTGTAATGCGATTCATCATTCGCACAGTTATGCTGATAGCACTGGTATGGATAGGATTATTACTCAGCGAATATGGCGTTTTGATTGGa                                                                                                                                                                                                                         <  1:156175/139‑1 (MQ=255)
                                                                                                                                          ttGCCGCGACGATTAAGCGAACATAAAAAGAGAAGAGGTTGTAATGCGATTCATCATTCGCACAGTTATGCTGAGAGCACTGGGATGGATAGGATTATTAATCAGCGAATATGGCGTTTTGATTGGAAGCCAAGAGAATGCCGCAGGATTAGGGTTGCAATGTACCTATCTGACTGCCAGAGGCAcc                                                                                                                                                             >  1:168674/1‑187 (MQ=255)
                                                                                                                                                cTACGATTAAGCGAACATAAAAAGAGAAGCGGTTGTAATGCGATTCATCATTCGCACAGTTATGCTGATAGCACTGGTATGGATAGGATTATTACTCAGCGAATATGGCGTTTTGATTGGAAGCAAAGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCCCGCTGTTACGCaaa                                                                                        >  1:283402/1‑250 (MQ=255)
                                                                                                                                                           cGAACATAAAAAGAGAAGAGGTTGTAATGCGATTCATCATTCGCACAGTTATGCTGATAGCACTGGTATGGATAGGATTATTACTCAGCGAATATGGCGTTTTg                                                                                                                                                                                                                               <  1:268120/104‑1 (MQ=255)
                                                                                                                                                                                             catcatTCNCNNNGNNATGCTGATAGCACTGGTATGGATAGGATTATTACTCAGCGAATANGGCGTTTTGATTGGANGCNCAGCNAATNCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGtt                                                                                                                           >  2:224097/1‑170 (MQ=255)
                                                                                                                                                                                             catcatTCGCACAGTTATGCTGATAGCTCTGGTATGGATAGGATTATTACTCAGCGAATATGGCGGTTTGATTGGAAGCAAAGAGAATGCCGCAGGATTAGGGTGGCAATTGACCTATCTGACTGCCAGATGCACCAGCACGGTACAATACTTTCACACTAAAAGTGGtt                                                                                                                           <  1:224097/170‑1 (MQ=255)
                                                                                                                                                                                                      nacaGTTNNGCTGATAGCACTGGTATGGATAGGATTATTACTCAGCGAATATGGCGTTTTGATTGGAAGCAAAGAGAATGCCGCAGGATTAGGTTTg                                                                                                                                                                                           <  2:423/96‑1 (MQ=255)
                                                                                                                                                                                                                  gATAGCACTGGTATGGATAGGATTATTACTCAGCGAATATGGCGTTTTGATTGGAAg                                                                                                                                                                                                                       <  2:1770/57‑1 (MQ=255)
                                                                                                                                                                                                                  gATAGCACTGGTATGGATAGGATTATTACTCAGCGAATATGGCGTTTTGATTGGAAg                                                                                                                                                                                                                       >  1:1770/1‑57 (MQ=255)
                                                                                                                                                                                                                                ngATAGGATTATTACTCAGCGAATATGGCGTTTTGATTGGAAGCAAAGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACtt                                                                                                                                            >  1:195934/2‑118 (MQ=255)
                                                                                                                                                                                                                                ggATAGGATTATTACTCAGCGAATATGGCGTTTTGATTGGAAGCAAAGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACtt                                                                                                                                            <  2:195934/118‑1 (MQ=255)
                                                                                                                                                                                                                                     ggATTATTACTCAGCGAATATGGCGTTTTGATTGGAAGCAAAGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGCGGCACCAGCACGGTCCACTACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCCCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATACTCTTATAGCAAGCGCGttt  >  1:190217/1‑251 (MQ=255)
                                                                                                                                                                                                                                              cTCAGCGGATATGGCGTTTTGATTGGAAGCAAAGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCCCGCTGTTACGCAAAAGCAat                                                                                  >  1:55831/1‑162 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          
ACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGTTAGTTAAACAGCGCCTAACTATGTCGATATTCGTGCTTTTTCGGAATGAGTCGCTTGCTTCAGCGCATATTGCCGCTACGATTAAGCGAACATAAAAAGAGAAGAGGTTGTAATGCGATTCATCATTCGCACAGTTATGCTGATAGCACTGGTATGGATAGGATTATTACTCAGCGGATATGGCGTTTTGATTGGAAGCAAAGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCCCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATACTCTTATAGCAAGCGCGTTT  >  NC_000913/1908744‑1909223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑