Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,888,5490GA68.2% 16.2 / 12.2 22P400S (CCA→TCA) fadDlong‑chain‑fatty‑acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (2/5);  new base A (8/7);  total (10/12)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.50e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCC  >  NC_000913/1888317‑1888753
                                                                                                                                                                                                                                        |                                                                                                                                                                                                            
tgctgcATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGCAACCAGAATCATGACTCTTTCACGATCGCCAATGCGCAGGAATACTTCTTCATCCATTACCGCGATGTCGCAGCTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGAAACAAAACTCACCCGGTTGACATGGTGATACTTCATTatcatcatc                                                                                                                                                                                            >  1:289767/1‑251 (MQ=255)
             cATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGATACTTCAGTATCATCATCATCCACCAGTTTg                                                                                                                                                                               <  1:86893/251‑1 (MQ=255)
                  tCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTATCATCATCATCCACCAGTTTgg                                                                                                                                                                              <  1:149306/247‑1 (MQ=255)
                                                                               cAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGGCCTTTGACACAAAGCTGACCCGGTTGACCTGGTGATACTTCATTATCATCATCATCCACCAGTGTTGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAAGATCATATGGGTTAACg                                                                                                             <  1:56467/251‑1 (MQ=255)
                                                                                           aTCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGATACTTCATTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATgn                                                                                                                                                <  1:96451/204‑2 (MQ=255)
                                                                                                         aTTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAgcgg                                                                                                  <  1:248416/236‑1 (MQ=255)
                                                                                                                cgATGTCGCCGGTGTGTAACCAGCCATTTGTGATGATTTCATCGGTATCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATGATCATCATCATCCACCAGTTTGGCTTGCTGCTACGGCGCCGGGAATCCGATGCTACCACTATGGTAATCTAGATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAg                                                                            <  1:242141/251‑1 (MQ=255)
                                                                                                                         cGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAACATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGTCGCACACTCGGTAAGGCCATAGCCTTCcagn                                                                   <  1:29481/251‑2 (MQ=255)
                                                                                                                            tgtgtNNACNNNCCNNTTTGATGATTTCATCGGTAGCATNGGGACGCTGCCAGTAACCCAGCATCACCTNCNGNCCTTNNACANAAAGCTCCCCNGGATGACCTGGTGATACTTCATTATCATCATCATCCACAAGTTTGGCTNCNGTCGACGGCACCGGCAAACCGATGCTACCACNATGATAATCAATATCCTATGGGTTNACGcnngancnnnnnnnn                                                                                              >  2:237225/1‑207 (MQ=255)
                                                                                                                            tgtgtAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTAGACACAAAGCTCACCCGGTTGACCTGGTGAGACTTCATTATCATCATCATCCACCAGTTTGTCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCt                                                                                                           <  1:237225/208‑1 (MQ=255)
                                                                                                                                        cATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAGCCCAGCATCACCTGCGGTCGTTTGACACAAAGCTCGCTCTGTTGACCGGGGGATACTTCATTATCATCATCATCCACCAGTTTGGCtt                                                                                                                                                                           <  1:148595/132‑1 (MQ=255)
                                                                                                                                                  gatTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGATNNTTCATTnnc                                                                                                                                                                                                  >  2:1984/1‑99 (MQ=255)
                                                                                                                                                  gatTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGATACTTCATTATCATCATCATCCACCAGTTTGGCTTCCGTc                                                                                                                                                                      <  1:1984/127‑1 (MQ=255)
                                                                                                                                                         tCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGATACTTCATTATCATCATCATCCCCCAg                                                                                                                                                                                   >  1:147308/1‑107 (MQ=255)
                                                                                                                                                                cATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGATACTTCATTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGccacn                            <  1:81390/251‑2 (MQ=255)
                                                                                                                                                                         gCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAACGCTCACCCGGTTGACCTGGTGATACTTCATTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATACTCAATATCCTATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTtgctgc                   >  1:46043/1‑251 (MQ=255)
                                                                                                                                                                          cTGCCAGTAACCGAGCATCACCTGCAGTCCTTTGACACAAAGCTCACCCGGTTGAACCGGTGGTACTTCCTTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGAGCAAACTCGGTAAGGCCATAGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTtgctgc                   >  1:107567/1‑250 (MQ=255)
                                                                                                                                                                                gTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCan                                                                                                                                          <  1:31407/125‑2 (MQ=255)
                                                                                                                                                                                   aCCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGATACTTCATTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAAc                                                                                                                                                      >  1:11907/1‑110 (MQ=255)
                                                                                                                                                                                                    gTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGATACTTCATTATCATCATCATCCACAAGTTTGGCTTCCGTCgccg                                                                                                                                                                  >  1:158616/1‑78 (MQ=255)
                                                                                                                                                                                                             cacaAAGCTCACCCGGTTGACCTGGTGGTACTTCATTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGcc  >  1:174640/1‑232 (MQ=255)
                                                                                                                                                                                                                   gCTCACCCGGTTGACCTGGTGATACTTCCTTATCATCATCATCCACAAGTTTGGCTTCCGTCGACGTCACCGGCAAACCGATGCTCCCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCAGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCCACGCTCTGcc                               >  1:206378/1‑197 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                            
TGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCC  >  NC_000913/1888317‑1888753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: