Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,634,661	0	C	G	57.1%	‑5.6 / 21.6	21	T75T (ACG→ACC)	tfaQ	putative tail fiber assembly protein TfaQ
Reads supporting (aligned to +/- strand):  ref base C (2/7);  new base G (7/5);  total (9/12)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGCCAT  >  NC_000913/1634450‑1634885                                                                                                                                                                                                                    |                                                                                                                                                                                                                                 gcgcAATCTGCTCACTGGCTCCCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCCGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGaaccc                                                                                                                                                                                           >  1:272420/1‑247 (MQ=11)  cgcAATATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGATTCTTCCGCCTCCCGGATCCGGACTAGTTTTTCTGCTTCCGTATCCTTCACCCCGGCTGCGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGCAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATgcct                                                                                                                                                                                          >  1:134495/1‑248 (MQ=11)          gCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACt                                                                                                                                                                                   <  1:283827/250‑1 (MQ=11)            tCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAAGTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGc                                                                                                                                                                                                    <  1:183297/231‑1 (MQ=11)                                   tttttttGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTGTCCTTCACCCAGGCTGTGCCGGTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAAGTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGTAATCCACGTCATAGACGGGTTTGCGCCGATGGTCTTCAACTAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAagccagcc                                                                                                                                                          <  1:312865/249‑1 (MQ=11)                                                         cGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCACTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATc                                                                                                                                   >  1:279277/1‑251 (MQ=11)                                                                           tCTGCTTCCGTATCCTTCACCCAGTCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTTCCCCGATGATCTTCATCGAGATGCGTCGAGGACTCAGCACTGTTGTAATCAACTACTAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCa                                                                                                                  <  1:307472/250‑1 (MQ=11)                                                                                                  ggCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAatatatn                                                                                          <  1:79650/251‑2 (MQ=14)                                                                                                      gtgCCGTTCCTCTTCTGAAACTCCCCTTCCGGTGTTAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAAGAAATAACGCTTCGCCGGAAGCCACGTCAGAAGCCGTTTTTCCCCTATGATCCTCAACGAGATGCCACGATGACTCATCACTGTTGAGAACAGCCACGAAGCCAGCCCCAATATCGGTCGTTGCAATATCGGTACGGTTTGCTGGCAGACCTGTATGAGGCGGAatatat                                                                                           <  1:167149/246‑1 (MQ=11)                                                                                                                              cctcccGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCCCGTCATAGACGGTTTTAcccc                                                                                                                                                                                                                        >  1:120024/5‑97 (MQ=11)                                                                                                                                 tCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATTACTCATCTCTGTTGAAAACAGCCGCGAAGCCAGCCGGAATATCTGGCGGTGTAGTATCGGTATTGTTAGCAAGCATACCTGTATGAGGCTGAATATATGCATCACCTTCGCCAATAAATTCATTAGtt                                                             <  1:162797/249‑1 (MQ=11)                                                                                                                                 nccGGTGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAag                                                                                                                                                                >  1:63017/2‑150 (MQ=11)                                                                                                                                          aaCCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCtn                                                                                                                     <  1:80517/184‑2 (MQ=17)                                                                                                                                                             gntaNGGACCNAGTTNAGAAATAAATAACGCNTCGCCGGATGCCACGTCATNNANGGTTTNAcccc                                                                                                                                                                                                                        <  2:120024/63‑1 (MQ=11)                                                                                                                                                               aaCGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCg                             >  1:116818/1‑251 (MQ=11)                                                                                                                                                                    aCCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcatcan                                                                                                                                                                                    <  1:204429/95‑2 (MQ=21)                                                                                                                                                                    aCCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCAc                                                                                                                                                                                    >  2:204429/1‑95 (MQ=34)                                                                                                                                                                               aaataaatAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATGTGGCGGTGCAATATCg                                                                                                                                  <  1:286453/134‑1 (MQ=18)                                                                                                                                                                                          gcgTCGCCGGAAGCCACGTCATAAAACGTTTTACCCCGATAATCTTCAACGAGATGCCACGATGACTCATAACTGTTGAAACCAGCCACGAAGCCAGCCGGAATATCTGGCGATGCAATATCGGTACTGTTGGCTGGCCGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAACACATTCATTAGGTCCTGCCAGCAGATTATAAATTTTTATGGACGTTGGGTGTTCACTCATTCTTAATGctat  >  1:234630/1‑248 (MQ=11)                                                                                                                                                                                            gTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACANngccagcn                                                                                                                                                          >  2:27454/1‑96 (MQ=11)                                                                                                                                                                                            gTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGcagg                                                                                                                    <  1:27454/135‑4 (MQ=11)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                 GCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGCCAT  >  NC_000913/1634450‑1634885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑