Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913376,9580GA58.3% ‑3.8 / 10.7 12A63T (GCG→ACG) yaiLDUF2058 domain‑containing protein YaiL
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base A (2/5);  total (5/7)
Fisher's exact test for biased strand distribution p-value = 5.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCTCAAAGCAGGATTAGTCACCAGCAAAAAAGCGGCGAAGGTGGAGAGAACGGCGAAAAAATCGCGCGTTCAGGCGCGTGAAGCTCGGGCGGCGGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACAGCTTAGCGAACAGCAAAAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAGGTGAAGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCG  >  NC_000913/376738‑377192
                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          
cGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCTCAAAGCAGGATTAGTCACCAGCAAAAAAGCGGCGAAGGTGGAGAGAACGGCGAAAAAATCGCGCGTTCAGGCGCGTGAAGCTCGGGCGGCGGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACAGCTTAGCGAACAGCAACAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAgg                                                                                                                                                                                                              >  1:44759/1‑251 (MQ=255)
                         gAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCTCAAAGCAGGATGAGTCACCAGCAAAAAAGCGGCGAAGGTGGAGAGAACGGCGAAAAAATCGCGCGGTCAGGGGCGTGAAGCGCGGGCGGCGGTAGAAGAAAATAAAAAGGCACAGCTGGAGCGTGATAAACAGCTTAGCGAACAGCAAAAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAgg                                                                                                                                                                                                              <  1:211401/226‑1 (MQ=255)
                                                    cAAGAGCAGTTGCTCAAAGCAGGATTAGTCACCAGCAAAAAAGCGGCGAAGGTGGAGAGAACGGCGAAAAAATCGCGCGTTCAGGCGCGTGAAGCTCGGGCGGCGGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACAGCGTAGCGAACAGCAAAAACAAGCGGCGTTGTCGAAAGAATATAAAGCTCAGGTGAAGCAGCTTATTGAAATGACCCGAATCACCATTGCCAATGGCGATATTgg                                                                                                                                                          <  1:235064/251‑1 (MQ=255)
                                                                                    cAGCAAAAAAGCGGCGAAGGTGGAGAGAACGGCGAAAAAATAGCGCGTTCAGGCGCGTGAAGCTCGGGCGGCGGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACAGCTTAGCGAACAGCAAAAACAAACGGCGTTGGCGAAAGAATATAAAGCTCAGGTGAAGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATg                                                                                                                                                                    >  1:121519/1‑209 (MQ=255)
                                                                                       caaaaccgcggcgAAGGTGGAGAGAACGGCGAAAAAATCGCGCGTTCAGGCGCGTGAAGCTCGGGCGGCGGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACAGCTTAGCGAACAGCAAAACCAAGCGGCGTTGGCGAAAGAata                                                                                                                                                                                                                         >  1:72434/8‑153 (MQ=255)
                                                                                        aaaaaaGCGGCGAAGGTGGAGAGAACGGCGAAAAAATCGCGCGTTCAGGCGCGTGAAGCTCGGGCGGCGGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACAGCTTAGCGAACAGCAAAAACAAACGGCGTTGGCGAAAGAATATAAAGCTCAGGTGAAGCAGCTTATTGAAATTAACCGAATCACCATTGCCAATggn                                                                                                                                                                  <  1:261059/207‑2 (MQ=255)
                                                                                               cggcgAAGGTGGAGAGAACGGCGAAAAAATCGCGCGTTCAGGCGCGTGAAGCTCGGGCGGCGGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACAGCTTAGCGAACAGCAAAAACAAACGGCGTTGGCGAAAGAATATAAAGCTCAGGTGAAGCAGCTTATTGAAATGAACCGAAGCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGtn                                                                                                               <  1:64862/251‑2 (MQ=255)
                                                                                                   gAAGGTGGAGAGAACGGCGAAAAAATCGCGCGTTCAGGCGCGTGAAGCTCGGGCGGCCGTAGAAGAAAATAAAAAGGCACAACTTGAACGTGATAAACCGCTTAGCGAACAGCACAAACAAACGGCGTTGGCGAACGAATATAAAGCTCAGGTGAAGCCGCTTATTGAACTGAACCGCATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGAt                                                                                                            >  1:284363/1‑250 (MQ=255)
                                                                                                            gagaACGGCGAAAAAATCGCGCGTTCAGGCGCGTGAAGCTCGGGCGGCGGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACAGCTTAGCGAACAGCAAAAACAAACGGCGTTGGCGAAAGAATATAAAGCTCAGGTGAAGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATTGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTcacac                                                                                                  <  1:313288/251‑1 (MQ=255)
                                                                                                                          aaTCGCGCGTTCAGGCGCGTGAAGCTCGGGCGGCGGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACAGCTTAGCGAACAGCAAAAACAAACGGCGTTGGCGAAAGAATATAAAGCTCAGGTGAAGCAGCTTATTGAAATGAAcc                                                                                                                                                                                      <  1:109666/153‑1 (MQ=255)
                                                                                                                                                           cggTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACAGCTTAGCGAACAGCAAAAACAAACGGCGTTGGCGAAAGAATATAAAGCTCAGGTGAAGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATAGTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACGCAGGCGCAGGTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTc                                                   <  1:167802/251‑1 (MQ=255)
                                                                                                                                                                nannnaAATANAAAGGCACAGCNTGAGCGTGATAAACAGCTTAGCGAACAGNAAAAACAAgcg                                                                                                                                                                                                                                          <  2:41994/62‑1 (MQ=255)
                                                                                                                                                                gaagaaAATAAAAAGGCACAGCTTGAGCGTGATAAACCGCTTAGCGAAAAGCTAAAACAAgcg                                                                                                                                                                                                                                          >  1:41994/1‑63 (MQ=255)
                                                                                                                                                                                                            cGAACAGCAAAAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAGGTGACGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCg  >  1:154816/1‑251 (MQ=255)
                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          
CGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCTCAAAGCAGGATTAGTCACCAGCAAAAAAGCGGCGAAGGTGGAGAGAACGGCGAAAAAATCGCGCGTTCAGGCGCGTGAAGCTCGGGCGGCGGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACAGCTTAGCGAACAGCAAAAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAGGTGAAGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCG  >  NC_000913/376738‑377192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑