Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,135,180	IS2 (–) +5 bp	coding (1346‑1350/2181 nt)	katG →	catalase/hydroperoxidase HPI

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4135180 =	1 (0.080)	12 (0.920)	11/290	0.1	92.0%	coding (1346/2181 nt)	katG	catalase/hydroperoxidase HPI
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 4135184	1 (0.080)	27 (2.070)	21/290	0.0	96.3%	coding (1350/2181 nt)	katG	catalase/hydroperoxidase HPI
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

GAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4134987‑4135184 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTAC  <  NC_000913/4499511‑4499280                                                                                                                                                                                                                                                                                                                                                                                                                                                GAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGC                                                                                                                                                                                     <  1:209547/251‑1     TCGAGAATATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCGGATCTGGCAAGAGCCGCTGCCGCAGCCGAGCTACAACCCGACCGAGCAGTACATTTGAACTGGCCCCTATATTGCCAGACATCTGTTATCACTTAACCCATTACAAGCCCG                                                                                                                                                                                  <  1:291210/250‑1                                        CAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAG                                                                                                                                              <  1:104755/251‑1                                                CNACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCC                                                                                                                                                                             >  1:253579/1‑212                                                CAACGAAGCCTTTGCCCGTGCCTGGCTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCACGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGCCATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGAT                                                                                                                                                                       >  1:123831/1‑218                                                                       GGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATG                                                                                                                <  1:129242/250‑1                                                                          TCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGC                                                                                                                                                                                                                                 <  1:142940/134‑1                                                                                     CACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAACGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTA                                                                                                                                                                                                         >  1:52257/1‑147                                                                                         GGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTCACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGAGGATGCCATTCGTTATAAT                                                                                                                 >  1:237949/1‑231                                                                                                                        GCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGC                                                                                                                                    <  1:158847/181‑1                                                                                                                                         AAGATCTGATCTGGCAAGATCCGCTGNCGCAGCNNATNTACAACCCGACCGAGCAGGNCANNNGNNNTNGCCCCNATNNTTCCAGACANCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGANCGATAACCCnn                                                                                                                                              >  2:76560/1‑152                                                                                                                                         AAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAG                                                                                                                                              <  1:76560/154‑1                                                                                                                                                GATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGAGTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCATATATTCCCGTGGCGAGCGAGAATCCAGCGCACTAGGCGGGTGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCC                                                                                 <  1:151710/208‑1                                                                                                                                                    TGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGACCAGGACCTTTAGATTTGCCTCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTAACGAGGAGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTAGGGCATGATACTGATGGAGTCACGCTTTAT                                  >  1:301581/1‑251                                                                                                                                                     GGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATTCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGG                                                             <  1:211511/223‑1                                                                                                                                                     GGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCC                                                                                                                                                >  1:133300/1‑140                                                                                                                                                      GCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGCGCAGGACATTTGACTTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACACATAACAAGCCCGCAGCCGAAGATCTCACCGTGGAGCGCGATAAACCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTACCCCGTTTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCG                                >  1:221554/1‑251                                                                                                                                                      GCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACACGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTACGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTG                                                                                                  >  1:317218/1‑185                                                                                                                                                       CAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGT                                                                                            >  1:253039/1‑190                                                                                                                                                               gcgGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTTGATGTGCCCCTATATTTCCAGACATCTGTTATCACTGAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGT                                                                                                                                                               <  1:210496/112‑1                                                                                                                                                                  GCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCTCTGCCGCAGATATTCCCGTGGC                                                                                                                                                            <  1:92045/115‑1                                                                                                                                                                          CGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATn                                                                                                                                                                                                       <  1:83241/64‑2                                                                                                                                                                                 CAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGTCATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCGGGTTTGGGCATGATACTGATGTAGTCAn                                         <  1:41453/215‑2                                                                                                                                                                                    aCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTAC  >  1:92760/2‑251                                                                                                                                                                                    CCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCAAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGAGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTAC  >  1:320587/1‑251                                                                                                                                                                                             GCAGGACATTTGGATTTGCCCCTATATTTACAGACATCTGTTATCACTTATCCCCTTAAAAGACCGCTGCCGCCGATATTCCCGTGGCGAGCGATAACCAAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCCAGGTTCTTTGCTGCCGT                                                                               >  1:282299/1‑165                                                                                                                                                                                                GGACATTTGGATTTGCCCCTATCTTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATAC                                                     >  1:92334/1‑188                                                                                                                                                                                                                                                                                                                                                                                                                                                GAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4134987‑4135184 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTAC  <  NC_000913/4499511‑4499280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.